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- PDB-4v63: Structural basis for translation termination on the 70S ribosome. -

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Basic information

Entry
Database: PDB / ID: 4v63
TitleStructural basis for translation termination on the 70S ribosome.
Components
  • (30S ribosomal protein ...) x 20
  • (50S ribosomal protein ...) x 29
  • 16S RRNA
  • 23S RRNA23S ribosomal RNA
  • 5S RRNA5S ribosomal RNA
  • P and E-site tRNA(fMet)
  • Peptide chain release factor 1
  • mRNAMessenger RNA
KeywordsRIBOSOME / Ribonucleoprotein / Ribosomal protein / RNA-binding / rRNA-binding / Metal-binding / Zinc-finger / tRNA-binding / Protein biosynthesis
Function / homology
Function and homology information


translation release factor activity, codon specific / large ribosomal subunit rRNA binding / large ribosomal subunit / small ribosomal subunit / 5S rRNA binding / transferase activity / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome ...translation release factor activity, codon specific / large ribosomal subunit rRNA binding / large ribosomal subunit / small ribosomal subunit / 5S rRNA binding / transferase activity / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / zinc ion binding / metal ion binding / cytoplasm
Similarity search - Function
Peptide chain release factor 1 / Peptide chain release factor / PCRF domain / PCRF / Peptide chain release factor class I superfamily / Prokaryotic-type class I peptide chain release factors signature. / Peptide chain release factor class I / RF-1 domain / 30S ribosomal protein Thx / 30S ribosomal protein Thx ...Peptide chain release factor 1 / Peptide chain release factor / PCRF domain / PCRF / Peptide chain release factor class I superfamily / Prokaryotic-type class I peptide chain release factors signature. / Peptide chain release factor class I / RF-1 domain / 30S ribosomal protein Thx / 30S ribosomal protein Thx / 30S ribosomal protein / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein L10 / : / Ribosomal protein S14, type Z / Ribosomal protein L31 type A / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L10-like domain superfamily / Ribosomal protein L10P / Ribosomal protein L10 / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein S14/S29 / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L32p, bacterial type / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L20 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S4, bacterial-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / : / Ribosomal protein L30, bacterial-type / Ribosomal protein L16 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / L28p-like / Ribosomal protein L20 / Ribosomal protein S16 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L21
Similarity search - Domain/homology
: / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein bTHX / Large ribosomal subunit protein bL32 ...: / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein bTHX / Large ribosomal subunit protein bL32 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein bL33 / Peptide chain release factor 1 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein bL25 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein bL31 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL35
Similarity search - Component
Biological speciesThermus thermophilus (bacteria)
Escherichia coli (E. coli)
MethodX-RAY DIFFRACTION / SYNCHROTRON / Resolution: 3.207 Å
AuthorsLaurberg, M. / Asahara, H. / Korostelev, A. / Zhu, J. / Trakhanov, S. / Noller, H.F.
CitationJournal: Nature / Year: 2008
Title: Structural basis for translation termination on the 70S ribosome
Authors: Laurberg, M. / Asahara, H. / Korostelev, A. / Zhu, J. / Trakhanov, S. / Noller, H.F.
History
DepositionMay 16, 2008Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jul 9, 2014Provider: repository / Type: Initial release
SupersessionDec 10, 2014ID: 3D5A, 3D5B, 3D5C, 3D5D
Revision 1.1Dec 10, 2014Group: Other
Revision 1.2Sep 20, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn / struct_ref_seq / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_PDB_ins_code / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_PDB_ins_code / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_ptnr1_PDB_ins_code / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_ref_seq.db_align_end / _struct_ref_seq_dif.details

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
AA: 16S RRNA
AZ: P and E-site tRNA(fMet)
AV: mRNA
AY: P and E-site tRNA(fMet)
AB: 30S ribosomal protein S2
AC: 30S ribosomal protein S3
AD: 30S ribosomal protein S4
AE: 30S ribosomal protein S5
AF: 30S ribosomal protein S6
AG: 30S ribosomal protein S7
AH: 30S ribosomal protein S8
AI: 30S ribosomal protein S9
AJ: 30S ribosomal protein S10
AK: 30S ribosomal protein S11
AL: 30S ribosomal protein S12
AM: 30S ribosomal protein S13
AN: 30S ribosomal protein S14
AO: 30S ribosomal protein S15
AP: 30S ribosomal protein S16
AQ: 30S ribosomal protein S17
AR: 30S ribosomal protein S18
AS: 30S ribosomal protein S19
AT: 30S ribosomal protein S20
AU: 30S ribosomal protein Thx
AX: Peptide chain release factor 1
BA: 23S RRNA
BB: 5S RRNA
BD: 50S ribosomal protein L2
BE: 50S ribosomal protein L3
BF: 50S ribosomal protein L4
BG: 50S ribosomal protein L5
BH: 50S ribosomal protein L6
BI: 50S ribosomal protein L9
BJ: 50S ribosomal protein L10
BN: 50S ribosomal protein L13
BO: 50S ribosomal protein L14
BP: 50S ribosomal protein L15
BQ: 50S ribosomal protein L16
BR: 50S ribosomal protein L17
BS: 50S ribosomal protein L18
BT: 50S ribosomal protein L19
BU: 50S ribosomal protein L20
BV: 50S ribosomal protein L21
BW: 50S ribosomal protein L22
BX: 50S ribosomal protein L23
BY: 50S ribosomal protein L24
BZ: 50S ribosomal protein L25
B0: 50S ribosomal protein L27
B1: 50S ribosomal protein L28
B2: 50S ribosomal protein L29
B3: 50S ribosomal protein L30
B4: 50S ribosomal protein L31
B5: 50S ribosomal protein L32
B6: 50S ribosomal protein L33
B7: 50S ribosomal protein L34
B8: 50S ribosomal protein L35
CA: 16S RRNA
CZ: P and E-site tRNA(fMet)
CV: mRNA
CY: P and E-site tRNA(fMet)
CB: 30S ribosomal protein S2
CC: 30S ribosomal protein S3
CD: 30S ribosomal protein S4
CE: 30S ribosomal protein S5
CF: 30S ribosomal protein S6
CG: 30S ribosomal protein S7
CH: 30S ribosomal protein S8
CI: 30S ribosomal protein S9
CJ: 30S ribosomal protein S10
CK: 30S ribosomal protein S11
CL: 30S ribosomal protein S12
CM: 30S ribosomal protein S13
CN: 30S ribosomal protein S14
CO: 30S ribosomal protein S15
CP: 30S ribosomal protein S16
CQ: 30S ribosomal protein S17
CR: 30S ribosomal protein S18
CS: 30S ribosomal protein S19
CT: 30S ribosomal protein S20
CU: 30S ribosomal protein Thx
CX: Peptide chain release factor 1
DD: 50S ribosomal protein L2
DE: 50S ribosomal protein L3
DF: 50S ribosomal protein L4
DG: 50S ribosomal protein L5
DH: 50S ribosomal protein L6
DI: 50S ribosomal protein L9
DJ: 50S ribosomal protein L10
DN: 50S ribosomal protein L13
DO: 50S ribosomal protein L14
DP: 50S ribosomal protein L15
DQ: 50S ribosomal protein L16
DR: 50S ribosomal protein L17
DS: 50S ribosomal protein L18
DT: 50S ribosomal protein L19
DU: 50S ribosomal protein L20
DV: 50S ribosomal protein L21
DW: 50S ribosomal protein L22
DX: 50S ribosomal protein L23
DY: 50S ribosomal protein L24
DZ: 50S ribosomal protein L25
D0: 50S ribosomal protein L27
D1: 50S ribosomal protein L28
D2: 50S ribosomal protein L29
D3: 50S ribosomal protein L30
D4: 50S ribosomal protein L31
D5: 50S ribosomal protein L32
D6: 50S ribosomal protein L33
D7: 50S ribosomal protein L34
D8: 50S ribosomal protein L35
DA: 23S RRNA
DB: 5S RRNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)4,627,2202693
Polymers4,564,325112
Non-polymers62,8962581
Water0
1
AA: 16S RRNA
AZ: P and E-site tRNA(fMet)
AV: mRNA
AY: P and E-site tRNA(fMet)
AB: 30S ribosomal protein S2
AC: 30S ribosomal protein S3
AD: 30S ribosomal protein S4
AE: 30S ribosomal protein S5
AF: 30S ribosomal protein S6
AG: 30S ribosomal protein S7
AH: 30S ribosomal protein S8
AI: 30S ribosomal protein S9
AJ: 30S ribosomal protein S10
AK: 30S ribosomal protein S11
AL: 30S ribosomal protein S12
AM: 30S ribosomal protein S13
AN: 30S ribosomal protein S14
AO: 30S ribosomal protein S15
AP: 30S ribosomal protein S16
AQ: 30S ribosomal protein S17
AR: 30S ribosomal protein S18
AS: 30S ribosomal protein S19
AT: 30S ribosomal protein S20
AU: 30S ribosomal protein Thx
AX: Peptide chain release factor 1
BA: 23S RRNA
BB: 5S RRNA
BD: 50S ribosomal protein L2
BE: 50S ribosomal protein L3
BF: 50S ribosomal protein L4
BG: 50S ribosomal protein L5
BH: 50S ribosomal protein L6
BI: 50S ribosomal protein L9
BJ: 50S ribosomal protein L10
BN: 50S ribosomal protein L13
BO: 50S ribosomal protein L14
BP: 50S ribosomal protein L15
BQ: 50S ribosomal protein L16
BR: 50S ribosomal protein L17
BS: 50S ribosomal protein L18
BT: 50S ribosomal protein L19
BU: 50S ribosomal protein L20
BV: 50S ribosomal protein L21
BW: 50S ribosomal protein L22
BX: 50S ribosomal protein L23
BY: 50S ribosomal protein L24
BZ: 50S ribosomal protein L25
B0: 50S ribosomal protein L27
B1: 50S ribosomal protein L28
B2: 50S ribosomal protein L29
B3: 50S ribosomal protein L30
B4: 50S ribosomal protein L31
B5: 50S ribosomal protein L32
B6: 50S ribosomal protein L33
B7: 50S ribosomal protein L34
B8: 50S ribosomal protein L35
hetero molecules


Theoretical massNumber of molelcules
Total (without water)2,312,9171318
Polymers2,282,16256
Non-polymers30,7551262
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
CA: 16S RRNA
CZ: P and E-site tRNA(fMet)
CV: mRNA
CY: P and E-site tRNA(fMet)
CB: 30S ribosomal protein S2
CC: 30S ribosomal protein S3
CD: 30S ribosomal protein S4
CE: 30S ribosomal protein S5
CF: 30S ribosomal protein S6
CG: 30S ribosomal protein S7
CH: 30S ribosomal protein S8
CI: 30S ribosomal protein S9
CJ: 30S ribosomal protein S10
CK: 30S ribosomal protein S11
CL: 30S ribosomal protein S12
CM: 30S ribosomal protein S13
CN: 30S ribosomal protein S14
CO: 30S ribosomal protein S15
CP: 30S ribosomal protein S16
CQ: 30S ribosomal protein S17
CR: 30S ribosomal protein S18
CS: 30S ribosomal protein S19
CT: 30S ribosomal protein S20
CU: 30S ribosomal protein Thx
CX: Peptide chain release factor 1
DD: 50S ribosomal protein L2
DE: 50S ribosomal protein L3
DF: 50S ribosomal protein L4
DG: 50S ribosomal protein L5
DH: 50S ribosomal protein L6
DI: 50S ribosomal protein L9
DJ: 50S ribosomal protein L10
DN: 50S ribosomal protein L13
DO: 50S ribosomal protein L14
DP: 50S ribosomal protein L15
DQ: 50S ribosomal protein L16
DR: 50S ribosomal protein L17
DS: 50S ribosomal protein L18
DT: 50S ribosomal protein L19
DU: 50S ribosomal protein L20
DV: 50S ribosomal protein L21
DW: 50S ribosomal protein L22
DX: 50S ribosomal protein L23
DY: 50S ribosomal protein L24
DZ: 50S ribosomal protein L25
D0: 50S ribosomal protein L27
D1: 50S ribosomal protein L28
D2: 50S ribosomal protein L29
D3: 50S ribosomal protein L30
D4: 50S ribosomal protein L31
D5: 50S ribosomal protein L32
D6: 50S ribosomal protein L33
D7: 50S ribosomal protein L34
D8: 50S ribosomal protein L35
DA: 23S RRNA
DB: 5S RRNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)2,314,3031375
Polymers2,282,16256
Non-polymers32,1411319
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)210.130, 454.390, 616.450
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(segid A23S or segid A5S or segid AL*) and not resid 2093:2196 and not resid 1051:1108
21(segid B23S or segid B5S or segid BL*) and not resid 2093:2196 and not resid 1051:1108
12segid A16S or segid AS* or segid AT*
22segid B16S or segid BS* or segid BT*
13segid APTN or segid AMRN
23segid BPTN or segid BMRN
14segid AETN
24segid BETN
15segid ARF1 and not resid 1:100
25segid BRF1 and not resid 1:100
16segid ARF1 and resid 1:100
26segid BRF1 and resid 1:100
17segid A23S and resid 2093:2196
27segid B23S and resid 2093:2196
18segid A23S and resid 1051:1108
28segid B23S and resid 1051:1108

NCS domain segments:
Dom-IDComponent-IDEns-IDAuth seq-IDLabel seq-ID
1112093 - 21962093 - 2196
2112093 - 21962093 - 2196
114-1-1
214-1-1
1151 - 1001 - 100
2151 - 1001 - 100
1161 - 1001 - 100
2161 - 1001 - 100
1172093 - 21962093 - 2196
2172093 - 21962093 - 2196
1181051 - 11081051 - 1108
2181051 - 11081051 - 1108

NCS ensembles :
ID
1
2
3
4
5
6
7
8

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Components

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RNA chain , 5 types, 12 molecules AACAAZAYCZCYAVCVBADABBDB

#1: RNA chain 16S RRNA /


Mass: 494985.938 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: GenBank: 155076
#2: RNA chain
P and E-site tRNA(fMet)


Mass: 24802.785 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli)
#3: RNA chain mRNA / Messenger RNA


Mass: 8862.456 Da / Num. of mol.: 2 / Source method: obtained synthetically
#25: RNA chain 23S RRNA / 23S ribosomal RNA


Mass: 941050.938 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27
#26: RNA chain 5S RRNA / 5S ribosomal RNA


Mass: 40152.945 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: GenBank: 48271

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30S ribosomal protein ... , 20 types, 40 molecules ABCBACCCADCDAECEAFCFAGCGAHCHAICIAJCJAKCKALCLAMCMANCNAOCOAPCP...

#4: Protein 30S ribosomal protein S2 /


Mass: 29317.703 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62662
#5: Protein 30S ribosomal protein S3 /


Mass: 26751.076 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62663
#6: Protein 30S ribosomal protein S4 /


Mass: 24373.447 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62664
#7: Protein 30S ribosomal protein S5 /


Mass: 17583.416 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62665
#8: Protein 30S ribosomal protein S6 /


Mass: 11988.753 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62666
#9: Protein 30S ribosomal protein S7 /


Mass: 18050.973 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62667
#10: Protein 30S ribosomal protein S8 /


Mass: 15868.570 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62668
#11: Protein 30S ribosomal protein S9 /


Mass: 14429.661 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62669
#12: Protein 30S ribosomal protein S10 /


Mass: 11954.968 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62653
#13: Protein 30S ribosomal protein S11 /


Mass: 13737.868 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62654
#14: Protein 30S ribosomal protein S12 /


Mass: 14821.621 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P61941
#15: Protein 30S ribosomal protein S13 /


Mass: 14338.861 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62655
#16: Protein 30S ribosomal protein S14 /


Mass: 7158.725 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62656
#17: Protein 30S ribosomal protein S15 /


Mass: 10578.407 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62657
#18: Protein 30S ribosomal protein S16 /


Mass: 10409.983 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62238
#19: Protein 30S ribosomal protein S17 /


Mass: 12324.670 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62658
#20: Protein 30S ribosomal protein S18 /


Mass: 10258.299 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62659
#21: Protein 30S ribosomal protein S19 /


Mass: 10605.464 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62660
#22: Protein 30S ribosomal protein S20 /


Mass: 11722.116 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62661
#23: Protein/peptide 30S ribosomal protein Thx / Ribosome


Mass: 3350.030 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62613

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Protein , 1 types, 2 molecules AXCX

#24: Protein Peptide chain release factor 1 / RF1


Mass: 40153.531 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72HB8

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50S ribosomal protein ... , 29 types, 58 molecules BDDDBEDEBFDFBGDGBHDHBIDIBJDJBNDNBODOBPDPBQDQBRDRBSDSBTDTBUDU...

#27: Protein 50S ribosomal protein L2 /


Mass: 30532.551 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I07
#28: Protein 50S ribosomal protein L3 /


Mass: 22450.213 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I04
#29: Protein 50S ribosomal protein L4 /


Mass: 23303.980 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I05
#30: Protein 50S ribosomal protein L5 /


Mass: 21075.623 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I16
#31: Protein 50S ribosomal protein L6 /


Mass: 19568.926 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I19
#32: Protein 50S ribosomal protein L9 /


Mass: 16436.205 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72GV5
#33: Protein 50S ribosomal protein L10 /


Mass: 18586.639 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72GS1
#34: Protein 50S ribosomal protein L13 /


Mass: 18534.008 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72IN1
#35: Protein 50S ribosomal protein L14 /


Mass: 13309.586 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I14
#36: Protein 50S ribosomal protein L15 /


Mass: 16319.142 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I23
#37: Protein 50S ribosomal protein L16 /


Mass: 15977.909 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I11
#38: Protein 50S ribosomal protein L17 /


Mass: 13750.148 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I33
#39: Protein 50S ribosomal protein L18 /


Mass: 12639.845 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I20
#40: Protein 50S ribosomal protein L19 /


Mass: 17217.867 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72JU9
#41: Protein 50S ribosomal protein L20 /


Mass: 13779.275 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72L76
#42: Protein 50S ribosomal protein L21 /


Mass: 11069.153 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72HR2
#43: Protein 50S ribosomal protein L22 /


Mass: 12808.055 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I09
#44: Protein 50S ribosomal protein L23 /


Mass: 10759.808 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I06
#45: Protein 50S ribosomal protein L24 /


Mass: 12085.611 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I15
#46: Protein 50S ribosomal protein L25 / / TL5 / General stress protein CTC


Mass: 23238.572 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72IA7
#47: Protein 50S ribosomal protein L27 /


Mass: 9501.061 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72HR3
#48: Protein 50S ribosomal protein L28 /


Mass: 11032.174 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72G84
#49: Protein 50S ribosomal protein L29 /


Mass: 8670.258 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I12
#50: Protein 50S ribosomal protein L30 /


Mass: 6799.126 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72I22
#51: Protein 50S ribosomal protein L31 /


Mass: 11274.987 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72JR0
#52: Protein 50S ribosomal protein L32 /


Mass: 6734.104 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P62652
#53: Protein 50S ribosomal protein L33 /


Mass: 6632.896 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72GW3
#54: Protein/peptide 50S ribosomal protein L34 /


Mass: 6132.449 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: P80340
#55: Protein 50S ribosomal protein L35 /


Mass: 7506.178 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Thermus thermophilus (bacteria) / Strain: HB27 / References: UniProt: Q72L77

-
Non-polymers , 2 types, 2581 molecules

#56: Chemical...
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 2577 / Source method: obtained synthetically / Formula: Mg
#57: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Zn

-
Details

Sequence detailsE.COLI NUMBERING IS USED FOR 5S, 16S AND 23S RRNA. P- AND E-TRNA (TRNA FMET) HAVE NUMBERING ...E.COLI NUMBERING IS USED FOR 5S, 16S AND 23S RRNA. P- AND E-TRNA (TRNA FMET) HAVE NUMBERING ACCORDING TO THE CANONICAL YEAST TRNA PHE. RF1 AND RIBOSOMAL PROTEINS HAVE NUMBERING BASED ON THE PREDICTED SEQUENCE POSITION OF THE START METHIONINE (POSITION 1).

-
Experimental details

-
Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

-
Sample preparation

CrystalDensity Matthews: 3.45 Å3/Da / Density % sol: 64 %
Crystal growTemperature: 296 K / Method: vapor diffusion, hanging drop / pH: 7
Details: 100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Components of the solutions
IDNameCrystal-IDSol-ID
1TRIS-OAc11
2KSCN11
3PEG20K11
4PEG20011
5DEOXY BIGCHAP11
6H2O11
7TRIS-OAc12
8KSCN12
9PEG20K12
10PEG20012
11DEOXY BIGCHAP12
12H2O12

-
Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 23-ID-D / Wavelength: 0.9537 Å
DetectorType: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Dec 17, 2007
Details: Si(111) Double Crystal Monochromator. Adjustable focusing mirrors in K-B geometry
RadiationMonochromator: Double crystal cryo-cooled Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9537 Å / Relative weight: 1
ReflectionResolution: 3.2→50 Å / Num. obs: 906561 / % possible obs: 95.9 % / Redundancy: 12.4 % / Biso Wilson estimate: 53.87 Å2 / Rmerge(I) obs: 0.271 / Rsym value: 0.271 / Net I/σ(I): 8.21
Reflection shellResolution: 3.2→3.3 Å / Redundancy: 7 % / Rmerge(I) obs: 0.795 / Mean I/σ(I) obs: 2.85 / Rsym value: 0.795 / % possible all: 89

-
Processing

Software
NameVersionClassification
PHENIX(phenix.refine)refinement
CNS1.2refinement
XDSdata reduction
XDSdata scaling
CNS1.2phasing
RefinementStarting model: PROTEIN DATA BANK ACCESSION CODES 2J00,2J01,2J02 AND 2J03.

2j00
PDB Unreleased entry

2j01
PDB Unreleased entry

2j02
PDB Unreleased entry

2j03
PDB Unreleased entry


Resolution: 3.207→49.901 Å / Occupancy max: 1 / Occupancy min: 1 / SU ML: 0.85 / σ(F): 2.03 / Phase error: 37.17 / Stereochemistry target values: ML
Details: REFINEMENT WAS PERFORMED IN CNS v1.2 AND IN PHENIX. STATISTICS AS CALCULATED BY PHENIX.
RfactorNum. reflection% reflection
Rfree0.319 8284 0.91 %
Rwork0.292 898277 -
obs0.292 906561 95.14 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 20.003 Å2 / ksol: 0.18 e/Å3
Displacement parametersBiso max: 328.96 Å2 / Biso mean: 153.726 Å2 / Biso min: 0.07 Å2
Baniso -1Baniso -2Baniso -3
1--36.555 Å2-0 Å2-0 Å2
2---30.132 Å2-0 Å2
3----27.284 Å2
Refinement stepCycle: LAST / Resolution: 3.207→49.901 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms96544 200836 2581 0 299961
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.003323298
X-RAY DIFFRACTIONf_angle_d0.708483154
X-RAY DIFFRACTIONf_chiral_restr0.03161162
X-RAY DIFFRACTIONf_plane_restr0.00226466
X-RAY DIFFRACTIONf_dihedral_angle_d18.797132000
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 30

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
3.207-3.2440.3762020.376211892139168
3.244-3.2820.4072860.398278062809289
3.282-3.3220.4192640.398278162808089
3.322-3.3640.4112780.394279602823889
3.364-3.4080.3952720.389280852835790
3.408-3.4550.349400.389283882842890
3.455-3.5040.3982500.385284042865491
3.504-3.5570.3952960.388283782867491
3.557-3.6120.4053130.394286362894992
3.612-3.6710.3981410.377290142915592
3.671-3.7350.4232180.365291942941293
3.735-3.8030.372980.359294872978594
3.803-3.8760.3792740.356298453011995
3.876-3.9550.3511620.351302763043896
3.955-4.0410.3722920.34305943088697
4.041-4.1350.3892980.337308873118599
4.135-4.2380.3483310.32310783140999
4.238-4.3520.3633350.3073124731582100
4.352-4.4810.3323020.2963137931681100
4.481-4.6250.3133230.283137531698100
4.625-4.790.3133130.2563147431787100
4.79-4.9820.2772970.243144831745100
4.982-5.2080.2773260.2233148031806100
5.208-5.4830.2493360.2123150031836100
5.483-5.8260.2772900.2083163731927100
5.826-6.2750.263240.1963153631860100
6.275-6.9050.2352930.1783172132014100
6.905-7.90.1983150.1633177432089100
7.9-9.9410.2222950.1863201932314100
9.941-49.9070.2513200.243265032970100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.3874-0.1070.11860.81810.08960.51670.024-0.09440.13430.09680.05310.0199-0.0089-0.022200.8708-0.08130.02550.86690.07420.7245-17.8463135.3136167.0381
20.14130.55020.1580.43740.29240.36750.036-0.19530.0893-0.08040.14450.6321-0.2236-0.2090.00011.27120.09230.25351.8481-0.09982.62-113.8353143.5341169.0372
30.3561-0.10230.01750.57590.07010.3099-0.0142-0.05560.06590.17590.00680.2629-0.0002-0.1422-01.1963-0.02820.01081.2314-0.011.3525-35.5043130.927172.7179
40.8576-0.2121-0.08380.35090.17610.57720.19350.1537-0.3391-0.5281-0.21980.74860.0155-0.457502.06740.2785-0.86041.5822-0.12632.4377-96.4748101.806169.6196
50.4195-0.298-0.01460.5602-0.03370.35540.24860.048-0.1862-0.5513-0.28130.53410.0681-0.2613-02.15540.2126-0.73381.7673-0.15082.5358-100.305107.683466.7937
60.3829-0.0176-0.03770.91330.25980.49230.08760.0687-0.212-0.3414-0.0023-0.0970.09620.0813-01.28150.0503-0.19581.0646-0.01591.1745-13.888868.754194.6443
70.18840.0296-0.08720.76290.19120.32190.10490.2496-0.0772-0.6498-0.12660.0285-0.0758-0.0639-01.85070.1499-0.2061.3136-0.05961.4512-20.422883.893268.0353
80.0186-0.03430.07750.0535-0.08340.0669-0.0655-0.33250.0697-0.1443-0.02140.3418-0.0752-0.6996-01.30870.1371-0.09621.5519-0.10541.7433-69.5372124.5086123.8446
90.0511-0.02030.0222-0.0013-0.00280.05080.2380.17290.042-0.31070.1588-0.0651-0.40640.1736-01.31780.2499-0.49711.77170.02331.7211-57.5479103.375986.3966
10-0.13380.08690.119-0.0784-0.0620.11050.21890.28030.1563-0.41540.00250.2812-0.2305-0.15130.00013.4413-0.3279-0.18122.84750.1323.0958-65.8811159.8732105.5435
110.0332-0.13940.1123-0.0846-0.1382-0.05540.20240.76860.1757-0.89650.21741.3116-0.5179-1.0888-0.00033.64690.2128-0.36263.04790.3463.3549-51.5766193.088989.171
120.05710.01870.02550.0103-0.03020.03740.13150.12520.1883-0.22520.12640.2084-0.0458-0.33310.00023.8288-0.2177-0.11894.19580.30054.1837-96.011464.5603166.302
130.1011-0.0298-0.00290.2804-0.16640.17790.0428-0.1044-0.55220.1046-0.18580.37850.1885-0.299-01.41220.1719-0.14271.3919-0.26021.6804-66.218690.5266121.6517
140.04040.0027-0.07130.10770.050.0619-0.0540.0089-0.048-0.0547-0.15720.04160.0747-0.0034-0.00022.91850.2313-0.59912.9426-0.26732.8899-86.929557.8026123.6979
150.4623-0.08710.16680.7212-0.20560.6290.001-0.0584-0.0839-0.18970.05770.02190.1353-0.0341-00.74210.0004-0.16610.7027-0.00220.7641-73.3888-57.177320.915
160.26860.04690.26460.3043-0.19570.29020.0410.04160.30640.15760.08280.4707-0.0034-0.5192-01.15780.04870.00192.14790.58862.6912-169.1548-54.241931.1831
170.4150.00850.08470.4745-0.11110.41360.0213-0.0682-0.0029-0.11060.09270.25110.1581-0.153801.1212-0.0101-0.12331.07350.11761.4416-91.4177-61.728516.7277
180.86060.3205-0.1590.6735-0.11530.6249-0.00540.06990.1907-0.19430.06570.4991-0.2894-0.5101.70640.6349-0.34631.66220.27872.4853-147.794345.0805-7.6377
190.2317-0.22560.10590.239-0.1010.4277-0.1789-0.14520.0546-0.07740.13860.4896-0.1151-0.131-01.91420.4113-0.29191.70850.16972.686-151.324147.9338-1.5882
200.4512-0.05220.10090.6555-0.26240.6653-0.00590.31960.224-0.494-0.0592-0.129-0.18960.21940.00011.5516-0.045-0.14991.13510.30181.6221-67.452416.9064-43.5612
210.2509-0.1243-0.03090.5391-0.12740.8006-0.08250.10310.4976-0.26370.0552-0.1161-0.37840.1093-01.669-0.1378-0.28531.19360.29952.1004-72.25243.3927-27.5804
220.85290.2050.67370.4140.68351.3133-0.8762-0.22080.18020.0174-0.06380.6803-0.3165-0.5516-0.45120.42570.36310.71461.13460.11791.6976-122.9249-10.974912.8658
230.07680.0039-0.04770.10150.00390.0185-0.0187-0.22410.24140.23590.0353-0.0226-0.34270.1567-01.36410.1619-0.10341.21810.08492.1939-109.602326.4314-7.5257
24-0.0985-0.0344-0.01550.09440.015-0.1199-0.0821-0.17550.17440.12710.0470.2087-0.4056-0.2961-03.10970.4183-0.37542.8330.21563.4365-117.535.779448.5656
250.0113-0.2319-0.1032-0.10650.1084-0.10990.0779-0.35930.61880.7573-0.24080.9877-0.4164-0.9462-0.00023.38520.30320.01242.7211-0.2383.4195-102.240820.583681.4546
26-0.00160.0130.02290.02790.0375-0.02270.2915-0.25350.47080.02-0.07090.153-0.5284-0.0581-0.00013.0181-0.0883-0.0642.94590.10383.2184-153.0086-49.7301-47.9384
270.25450.0992-0.13020.0831-0.19840.1225-0.34760.25550.02830.02660.23610.0599-0.2145-0.2346-01.4062-0.0711-0.25791.34810.20391.7508-120.5047-7.9901-21.0656
280.09640.07730.0340.0787-0.08940.13820.23540.1807-0.2414-0.26170.1303-0.14750.35320.2870.00043.47811.02340.09453.0042-0.12153.747-142.4119-8.1118-53.0722
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1((chain BA) and not (resid 2093:2196 or resid 1051:1108))
2X-RAY DIFFRACTION2(chain AA)
3X-RAY DIFFRACTION3(chain BD or chain BE or chain BF or chain BG or chain BH or chain BI or chain BJ or chain BN or chain BO or chain BP or chain BQ or chain BS or chain BT or chain BU or chain BV or chain BW or chain BX of chain BY of chain BZ or chain B0 or chain B1 or chain B2 or chain B3 or chain B4 or chain B5 or chain B6 or chain B7 or chain B8)
4X-RAY DIFFRACTION4((chain AA and resid 925:1391))
5X-RAY DIFFRACTION5(chain AC or chain AG or chain AI or chain AJ or chain AM or chain AN or chain AS)
6X-RAY DIFFRACTION6(chain A5 and not resid 925:1391)
7X-RAY DIFFRACTION7(chain AB or chain AD or chain AE or chain AF or chain AH or chain AK of chain AL or chain AO or chain AP or chain AQ or chain AR or chain AT or chain BU)
8X-RAY DIFFRACTION8(chain AY)
9X-RAY DIFFRACTION9(chain AV)
10X-RAY DIFFRACTION10(chain AZ)
11X-RAY DIFFRACTION11(chain BA and resid 2093:2196)
12X-RAY DIFFRACTION12(chain BA and resid 1051:1108)
13X-RAY DIFFRACTION13(chain AX and not resid 1:100)
14X-RAY DIFFRACTION14(chain AZ and resid 1:100)
15X-RAY DIFFRACTION15((chain DA) and not (resid 2093:2196 or resid 1051:1108))
16X-RAY DIFFRACTION16(chain CA)
17X-RAY DIFFRACTION17(chain DD or chain DE or chain DF or chain DG or chain DH or chain DI or chain DJ or chain DN or chain DO or chain DP or chain DQ or chain DS or chain DT or chain DU or chain DV or chain DW or chain DX of chain DY of chain DZ or chain D0 or chain D1 or chain D2 or chain D3 or chain D4 or chain D5 or chain D6 or chain D7 or chain D8)
18X-RAY DIFFRACTION18((chain CA and resid 925:1391))
19X-RAY DIFFRACTION19(chain CC or chain CG or chain CI or chain CJ or chain CM or chain CN or chain CS)
20X-RAY DIFFRACTION20(chain C5 and not resid 925:1391)
21X-RAY DIFFRACTION21(chain CB or chain CD or chain CE or chain CF or chain CH or chain CK of chain CL or chain CO or chain CP or chain CQ or chain CR or chain CT or chain DU)
22X-RAY DIFFRACTION22(chain CY)
23X-RAY DIFFRACTION23(chain CV)
24X-RAY DIFFRACTION24(chain CZ)
25X-RAY DIFFRACTION25(chain DA and resid 2093:2196)
26X-RAY DIFFRACTION26(chain DA and resid 1051:1108)
27X-RAY DIFFRACTION27(chain CX and not resid 1:100)
28X-RAY DIFFRACTION28(chain CZ and resid 1:100)

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Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

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