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- PDB-4v43: Structural and mechanistic basis for allostery in the bacterial c... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4v43 | |||||||||
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Title | Structural and mechanistic basis for allostery in the bacterial chaperonin GroEL | |||||||||
![]() | GROEL PROTEIN | |||||||||
![]() | CHAPERONE / Wild Type GroEL / ALLOSTERY | |||||||||
Function / homology | ![]() GroEL-GroES complex / chaperonin ATPase / mitochondrial unfolded protein response / protein import into mitochondrial intermembrane space / virion assembly / chaperone cofactor-dependent protein refolding / positive regulation of interferon-alpha production / isomerase activity / ATP-dependent protein folding chaperone / response to radiation ...GroEL-GroES complex / chaperonin ATPase / mitochondrial unfolded protein response / protein import into mitochondrial intermembrane space / virion assembly / chaperone cofactor-dependent protein refolding / positive regulation of interferon-alpha production / isomerase activity / ATP-dependent protein folding chaperone / response to radiation / positive regulation of interleukin-6 production / positive regulation of type II interferon production / unfolded protein binding / protein folding / positive regulation of T cell activation / protein-folding chaperone binding / response to heat / protein refolding / magnesium ion binding / ATP hydrolysis activity / ATP binding / identical protein binding / membrane / cytosol Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Wang, J. | |||||||||
![]() | ![]() Title: A GroEL/GroES complex structure revisited: the structure-based mechanism of ATP hydrolysis Authors: Wang, J. / Boisvert, D.C. #1: ![]() Title: The 2.4 A Crystal Structure of the Bacterial Chaperonin GroEL Complexed with ATP Gamma S Authors: Boisvert, D.C. / Wang, J. / Otwinowski, Z. / Horwich, A.L. / Sigler, P.B. #2: ![]() Title: The Crystal Structure of the Bacterial Chaperonin GroEL at 2.8 A Authors: Braig, K. / Otwinowski, Z. / Hegde, R. / Boisvert, D.C. / Joachimiak, A. / Horwich, A.L. / Sigler, P.B. #3: ![]() Title: Conformational Variability in the Refined Structure of the Chaperonin GroEL at 2.8 A Resolution Authors: Braig, K. / Adams, P.D. / Brunger, A.T. #4: ![]() Title: The Crystal Structure of the Asymmetric GroEL-GroES-(ADP)7 Chaperonin Complex Authors: Xu, Z. / Horwich, A.L. / Sigler, P.B. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 2.5 MB | Display | ![]() |
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PDB format | ![]() | Display | ![]() | |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 738.1 KB | Display | ![]() |
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Full document | ![]() | 1.4 MB | Display | |
Data in XML | ![]() | 542.1 KB | Display | |
Data in CIF | ![]() | 731.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1kp8S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 57158.457 Da / Num. of mol.: 28 / Mutation: D398A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.35 Å3/Da / Density % sol: 63.25 % |
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Crystal grow | pH: 7 / Details: pH 7.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Detector: CCD / Date: Nov 15, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3.5→20 Å / Num. obs: 217987 / % possible obs: 85.1 % / Observed criterion σ(I): -3 / Redundancy: 11.02 % / Rmerge(I) obs: 0.114 |
Reflection shell | Resolution: 3.5→3.62 Å / Rmerge(I) obs: 0.337 / Mean I/σ(I) obs: 1.396 / % possible all: 51.4 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1kp8 Resolution: 3.52→20 Å / Rfactor Rfree error: 0.003 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 11.4049 Å2 / ksol: 0.231794 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 80.7 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.52→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.52→3.72 Å / Rfactor Rfree error: 0.015 / Total num. of bins used: 6
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Xplor file |
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