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- PDB-4uov: The structure of a tetrameric alpha-carbonic anhydrase from Therm... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4uov | ||||||
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Title | The structure of a tetrameric alpha-carbonic anhydrase from Thermovibrio ammonificans reveals a core formed around intermolecular disulfides, which contribute to its thermostability. | ||||||
![]() | CARBONATE DEHYDRATASE | ||||||
![]() | LYASE / CARBONIC ANHYDRASE / THERMOPHILIC / ACETAZOLAMIDE / CARBON DIOXIDE CAPTURE / THERMAL STABILITY. | ||||||
Function / homology | ![]() regulation of pH / carbonic anhydrase / carbonate dehydratase activity / carbon dioxide transport / zinc ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | James, P. / Isupov, M. / Sayer, C. / Berg, S. / Lioliou, M. / Kotlar, H. / Littlechild, J. | ||||||
![]() | ![]() Title: The Structure of a Tetrameric [Alpha]-Carbonic Anhydrase from Thermovibrio Ammonificans Reveals a Core Formed Around Intermolecular Disulfides that Contribute to its Thermostability Authors: James, P. / Isupov, M.N. / Sayer, C. / Saneei, V. / Berg, S. / Lioliou, M. / Kotlar, H. / Littlechild, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 362.8 KB | Display | ![]() |
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PDB format | ![]() | 297.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 3.7 MB | Display | ![]() |
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Full document | ![]() | 3.8 MB | Display | |
Data in XML | ![]() | 82 KB | Display | |
Data in CIF | ![]() | 116.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4c3tSC ![]() 4coqC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
-Protein , 1 types, 6 molecules ABCDEF
#1: Protein | Mass: 27725.900 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Details: DSMZ / Source: (natural) ![]() |
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-Non-polymers , 11 types, 1976 molecules ![](data/chem/img/ZN.gif)
![](data/chem/img/AZM.gif)
![](data/chem/img/PE3.gif)
![](data/chem/img/B3P.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/TLA.gif)
![](data/chem/img/PGE.gif)
![](data/chem/img/PG5.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/AZM.gif)
![](data/chem/img/PE3.gif)
![](data/chem/img/B3P.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/TLA.gif)
![](data/chem/img/PGE.gif)
![](data/chem/img/PG5.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-ZN / #3: Chemical | ChemComp-AZM / #4: Chemical | #5: Chemical | ChemComp-B3P / #6: Chemical | ChemComp-SO4 / #7: Chemical | ChemComp-CL / #8: Chemical | ChemComp-PEG / #9: Chemical | #10: Chemical | ChemComp-PGE / #11: Chemical | ChemComp-PG5 / | #12: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.66 Å3/Da / Density % sol: 66 % / Description: NONE |
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Crystal grow | pH: 5.5 Details: 9% ETHYLENE GLYCOL, 9 % PEG8000, 10 MM CARBOXYLIC ACIDS ADDITIVE MIXTURE, 100MM MES PH 6.5. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS / Detector: PIXEL / Date: Aug 10, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92 Å / Relative weight: 1 |
Reflection | Resolution: 1.85→42.01 Å / Num. obs: 206954 / % possible obs: 99.6 % / Observed criterion σ(I): 0 / Redundancy: 7.5 % / Rmerge(I) obs: 0.1 / Net I/σ(I): 13.7 |
Reflection shell | Resolution: 1.85→1.9 Å / Redundancy: 7.4 % / Rmerge(I) obs: 0.95 / Mean I/σ(I) obs: 2.4 / % possible all: 99.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 4C3T Resolution: 1.85→42.01 Å / Cor.coef. Fo:Fc: 0.965 / Cor.coef. Fo:Fc free: 0.952 / SU B: 2.657 / SU ML: 0.077 / Cross valid method: THROUGHOUT / ESU R: 0.104 / ESU R Free: 0.104 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY. LOCAL NCS RESTRICTIONS WERE APPLIED THROUGHOUT REFINEMENT
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28.828 Å2
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Refinement step | Cycle: LAST / Resolution: 1.85→42.01 Å
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Refine LS restraints |
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