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Open data
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Basic information
Entry | Database: PDB / ID: 4qh7 | ||||||
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Title | LC8 - Ana2 (159-168) Complex | ||||||
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![]() | MOTOR PROTEIN / LC8 fold Dimer / Target dimerization / Ana2 / Cellular | ||||||
Function / homology | ![]() ciliary basal body organization / spermatid nucleus elongation / chaeta morphogenesis / positive regulation of neuron remodeling / Macroautophagy / Aggrephagy / centriole-centriole cohesion / wing disc development / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / COPI-mediated anterograde transport ...ciliary basal body organization / spermatid nucleus elongation / chaeta morphogenesis / positive regulation of neuron remodeling / Macroautophagy / Aggrephagy / centriole-centriole cohesion / wing disc development / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / COPI-mediated anterograde transport / COPI-independent Golgi-to-ER retrograde traffic / microtubule anchoring at centrosome / centriole assembly / chaeta development / sperm individualization / imaginal disc-derived wing morphogenesis / asymmetric neuroblast division / Neutrophil degranulation / dynein complex / dynein light intermediate chain binding / cytoplasmic dynein complex / dynein intermediate chain binding / oogenesis / establishment of mitotic spindle orientation / centriole replication / actin filament bundle assembly / centriole / ciliary basal body / disordered domain specific binding / spermatogenesis / microtubule / centrosome / protein homodimerization activity / protein-containing complex / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Slevin, L.K. / Romes, E.R. / Slep, K.C. | ||||||
![]() | ![]() Title: The Mechanism of Dynein Light Chain LC8-mediated Oligomerization of the Ana2 Centriole Duplication Factor. Authors: Slevin, L.K. / Romes, E.M. / Dandulakis, M.G. / Slep, K.C. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 95.2 KB | Display | ![]() |
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PDB format | ![]() | 73.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 457.3 KB | Display | ![]() |
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Full document | ![]() | 459 KB | Display | |
Data in XML | ![]() | 18.4 KB | Display | |
Data in CIF | ![]() | 27 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4qh8C ![]() 2pg1S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 10800.304 Da / Num. of mol.: 4 / Fragment: LC8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein/peptide | Mass: 1283.365 Da / Num. of mol.: 4 / Fragment: Ana2 T159-P168 / Source method: obtained synthetically Details: Sequence T159-P168 occurs naturally in Drosophila melanogaster. Peptide synthesized is NYTICAGTQTDP with C-terminal amide. Source: (synth.) ![]() ![]() #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.26 Å3/Da / Density % sol: 45.52 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 0.3M magnesium acetate, 0.1M sodium cacodylate, 26% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Sep 14, 2012 |
Radiation | Monochromator: Double crystal - liquid nitrogen-cooled / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.83→50 Å / Num. all: 108273 / Num. obs: 37488 / % possible obs: 95.1 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Rsym value: 0.08 / Net I/σ(I): 13.7 |
Reflection shell | Resolution: 1.83→1.9 Å / Mean I/σ(I) obs: 2.4 / Rsym value: 0.37 / % possible all: 95.2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2PG1 Resolution: 1.829→44.629 Å / SU ML: 0.15 / σ(F): 0 / Phase error: 19.33 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.829→44.629 Å
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Refine LS restraints |
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LS refinement shell |
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