negative regulation of cell-cell adhesion mediated by integrin / lymphocyte migration into lymph node / chemokine (C-C motif) ligand 21 signaling pathway / positive regulation of termination of RNA polymerase II transcription / monoatomic cation homeostasis / negative regulation of pancreatic juice secretion / protein insertion into ER membrane by stop-transfer membrane-anchor sequence / positive regulation of mitotic cytokinesis / negative regulation of sodium ion transport / monoatomic ion homeostasis ...negative regulation of cell-cell adhesion mediated by integrin / lymphocyte migration into lymph node / chemokine (C-C motif) ligand 21 signaling pathway / positive regulation of termination of RNA polymerase II transcription / monoatomic cation homeostasis / negative regulation of pancreatic juice secretion / protein insertion into ER membrane by stop-transfer membrane-anchor sequence / positive regulation of mitotic cytokinesis / negative regulation of sodium ion transport / monoatomic ion homeostasis / regulation of mRNA export from nucleus / positive regulation of potassium ion import across plasma membrane / regulation of sodium ion transmembrane transport / intracellular chloride ion homeostasis / negative regulation of heterotypic cell-cell adhesion / positive regulation of T cell chemotaxis / positive regulation of systemic arterial blood pressure / non-membrane-bounded organelle assembly / potassium ion homeostasis / cellular response to chemokine / potassium channel inhibitor activity / protein serine/threonine kinase inhibitor activity / negative regulation of leukocyte cell-cell adhesion / cellular hyperosmotic response / regulation of monoatomic cation transmembrane transport / cell volume homeostasis / negative regulation of protein localization to plasma membrane / intracellular non-membrane-bounded organelle / protein kinase activator activity / sodium ion transmembrane transport / GABA-ergic synapse / phosphatase binding / monoatomic ion transport / regulation of sodium ion transport / negative regulation of protein ubiquitination / cellular response to calcium ion / negative regulation of autophagy / molecular condensate scaffold activity / modulation of chemical synaptic transmission / mitotic spindle / regulation of blood pressure / positive regulation of angiogenesis / positive regulation of canonical Wnt signaling pathway / heart development / T cell receptor signaling pathway / non-specific serine/threonine protein kinase / protein kinase activity / intracellular signal transduction / protein phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / protein kinase binding / magnesium ion binding / signal transduction / protein-containing complex / ATP binding / membrane / nucleus / cytoplasm / cytosol 類似検索 - 分子機能
Serine/threonine-protein kinase OSR1/WNK, CCT domain / Oxidative-stress-responsive kinase 1 C-terminal domain / Transferase(Phosphotransferase) domain 1 / Transferase(Phosphotransferase); domain 1 / Phosphorylase Kinase; domain 1 / Phosphorylase Kinase; domain 1 / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain ...Serine/threonine-protein kinase OSR1/WNK, CCT domain / Oxidative-stress-responsive kinase 1 C-terminal domain / Transferase(Phosphotransferase) domain 1 / Transferase(Phosphotransferase); domain 1 / Phosphorylase Kinase; domain 1 / Phosphorylase Kinase; domain 1 / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily / 2-Layer Sandwich / Orthogonal Bundle / Mainly Alpha / Alpha Beta 類似検索 - ドメイン・相同性
BROMIDE ION / Serine/threonine-protein kinase WNK1 類似検索 - 構成要素
解像度: 3.5→43.409 Å / Cor.coef. Fo:Fc: 0.904 / Cor.coef. Fo:Fc free: 0.88 / SU B: 87.762 / SU ML: 0.633 / Isotropic thermal model: ISOTROPIC / 交差検証法: THROUGHOUT / σ(I): 2 / ESU R Free: 0.882 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
Rfactor
反射数
%反射
Selection details
Rfree
0.29743
364
10 %
RANDOM
Rwork
0.2428
-
-
-
obs
0.24827
3290
99.37 %
-
all
-
3680
-
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK
原子変位パラメータ
Biso mean: 114.639 Å2
Baniso -1
Baniso -2
Baniso -3
1-
-0.31 Å2
-0 Å2
-0.05 Å2
2-
-
0.58 Å2
0 Å2
3-
-
-
-0.26 Å2
精密化ステップ
サイクル: LAST / 解像度: 3.5→43.409 Å
タンパク質
核酸
リガンド
溶媒
全体
原子数
2180
0
1
9
2190
拘束条件
Refine-ID
タイプ
Dev ideal
Dev ideal target
数
X-RAY DIFFRACTION
r_bond_refined_d
0.004
0.019
2222
X-RAY DIFFRACTION
r_angle_refined_deg
0.753
1.969
2985
X-RAY DIFFRACTION
r_dihedral_angle_1_deg
3.369
5
270
X-RAY DIFFRACTION
r_dihedral_angle_2_deg
30.096
23.505
97
X-RAY DIFFRACTION
r_dihedral_angle_3_deg
13.214
15
431
X-RAY DIFFRACTION
r_dihedral_angle_4_deg
12.228
15
16
X-RAY DIFFRACTION
r_chiral_restr
0.049
0.2
329
X-RAY DIFFRACTION
r_gen_planes_refined
0.002
0.021
1628
X-RAY DIFFRACTION
r_mcbond_it
0.718
7.311
1083
X-RAY DIFFRACTION
r_mcangle_it
1.33
10.967
1352
X-RAY DIFFRACTION
r_scbond_it
0.537
7.355
1139
X-RAY DIFFRACTION
r_long_range_B_refined
4.581
60.341
3326
LS精密化 シェル
解像度: 3.5→3.591 Å / Total num. of bins used: 20
Rfactor
反射数
%反射
Rfree
0.27
25
-
Rwork
0.265
233
-
obs
-
-
100 %
精密化 TLS
手法: refined / Origin x: 13.3208 Å / Origin y: 0.3161 Å / Origin z: 17.265 Å