+Open data
-Basic information
Entry | Database: PDB / ID: 4psx | ||||||
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Title | Crystal structure of histone acetyltransferase complex | ||||||
Components |
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Keywords | HISTONE/TRANSFERASE / HAT WD40 / acetyltransferase / AcCoA / Phosphorylation / HISTONE-TRANSFERASE complex | ||||||
Function / homology | Function and homology information sexual sporulation resulting in formation of a cellular spore / HDMs demethylate histones / HATs acetylate histones / Rpd3L complex / HDACs deacetylate histones / Rpd3L-Expanded complex / Condensation of Prophase Chromosomes / global genome nucleotide-excision repair / RNA polymerase I upstream activating factor complex / histone H4 acetyltransferase activity ...sexual sporulation resulting in formation of a cellular spore / HDMs demethylate histones / HATs acetylate histones / Rpd3L complex / HDACs deacetylate histones / Rpd3L-Expanded complex / Condensation of Prophase Chromosomes / global genome nucleotide-excision repair / RNA polymerase I upstream activating factor complex / histone H4 acetyltransferase activity / SUMOylation of chromatin organization proteins / replication fork protection complex / RMTs methylate histone arginines / positive regulation of transcription by RNA polymerase I / nucleolar large rRNA transcription by RNA polymerase I / subtelomeric heterochromatin formation / rRNA transcription / histone acetyltransferase complex / CENP-A containing nucleosome / histone acetyltransferase activity / histone acetyltransferase / nucleosome assembly / structural constituent of chromatin / nucleosome / chromatin organization / histone binding / chromosome, telomeric region / chromatin remodeling / protein heterodimerization activity / DNA repair / chromatin binding / regulation of DNA-templated transcription / DNA binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) Saccharomyces cerevisiae S288c (yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.509 Å | ||||||
Authors | Yang, M. / Li, Y. | ||||||
Citation | Journal: Genes Dev. / Year: 2014 Title: Hat2p recognizes the histone H3 tail to specify the acetylation of the newly synthesized H3/H4 heterodimer by the Hat1p/Hat2p complex Authors: Li, Y. / Zhang, L. / Liu, T. / Chai, C. / Fang, Q. / Wu, H. / Agudelo garcia, P.A. / Han, Z. / Zong, S. / Yu, Y. / Zhang, X. / Parthun, M.R. / Chai, J. / Xu, R.M. / Yang, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4psx.cif.gz | 601.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4psx.ent.gz | 501.3 KB | Display | PDB format |
PDBx/mmJSON format | 4psx.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ps/4psx ftp://data.pdbj.org/pub/pdb/validation_reports/ps/4psx | HTTPS FTP |
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-Related structure data
Related structure data | 4pswSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
-Histone acetyltransferase type B ... , 2 types, 4 molecules ADBE
#1: Protein | Mass: 37566.344 Da / Num. of mol.: 2 / Fragment: UNP residues 7-319 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Strain: ATCC 204508 / S288c / Gene: HAT1, LPA16W, YP8132.12, YPL001W / Production host: Escherichia coli (E. coli) / References: UniProt: Q12341, histone acetyltransferase #2: Protein | Mass: 45107.902 Da / Num. of mol.: 2 / Fragment: UNP residues 8-389 / Mutation: V143T Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Strain: ATCC 204508 / S288c / Gene: HAT2, YEL056W / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P39984 |
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-Protein/peptide , 2 types, 4 molecules CFYP
#3: Protein/peptide | Mass: 5093.079 Da / Num. of mol.: 2 / Fragment: UNP residues 2-49 / Mutation: I21V Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Strain: ATCC 204508 / S288c / Gene: HHF1, YBR009C, YBR0122, HHF2, YNL030W, N2752 / Production host: Escherichia coli (E. coli) / References: UniProt: P02309 #4: Protein/peptide | Mass: 1565.797 Da / Num. of mol.: 2 / Fragment: UNP residues 2-16 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae S288c (yeast) / Strain: ATCC 204508 / S288c / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P61830 |
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-Non-polymers , 3 types, 609 molecules
#5: Chemical | #6: Chemical | ChemComp-SO4 / | #7: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.7 Å3/Da / Density % sol: 66.75 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 0.2M potassium sodium tartrate, 20%(w/v) polyethylene glycol 3,350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U / Wavelength: 0.9793 Å |
Detector | Type: RIGAKU / Detector: IMAGE PLATE / Date: Jun 1, 2012 |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→38.6 Å / Num. all: 66418 / Num. obs: 66418 / % possible obs: 75.67 % / Observed criterion σ(F): 1.98 / Observed criterion σ(I): 1.98 |
Reflection shell | Resolution: 2.5→2.55 Å / % possible all: 75.67 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4PSW Resolution: 2.509→38.6 Å / SU ML: 0.23 / σ(F): 1.98 / Phase error: 24.6 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.509→38.6 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 24
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Refinement TLS params. | Method: refined / Origin x: -13.3471 Å / Origin y: -60.3416 Å / Origin z: -15.4922 Å
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Refinement TLS group | Selection details: all |