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Open data
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Basic information
Entry | Database: PDB / ID: 4p37 | ||||||
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Title | Crystal structure of the Megavirus polyadenylate synthase | ||||||
![]() | (Putative poly(A) polymerase catalytic ...) x 2 | ||||||
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Function / homology | Poly(A) polymerase catalytic subunit / DI(HYDROXYETHYL)ETHER / Putative poly(A) polymerase catalytic subunit![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Priet, S. / Lartigue, A. / Claverie, J.M. / Abergel, C. | ||||||
![]() | ![]() Title: mRNA maturation in giant viruses: variation on a theme. Authors: Priet, S. / Lartigue, A. / Debart, F. / Claverie, J.M. / Abergel, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 231.6 KB | Display | ![]() |
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PDB format | ![]() | 192.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Details | Gel filtration confirms the dimerization of the protein in solution |
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Components
-Putative poly(A) polymerase catalytic ... , 2 types, 2 molecules AB
#1: Protein | Mass: 63666.695 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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#2: Protein | Mass: 63707.727 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
-Non-polymers , 4 types, 592 molecules ![](data/chem/img/PEG.gif)
![](data/chem/img/TRS.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/TRS.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | ChemComp-PEG / ![]() #4: Chemical | ChemComp-TRS / | ![]() #5: Chemical | ![]() #6: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.55 Å3/Da / Density % sol: 51.85 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.8 / Details: 0.1M Tris-HCl, PEG 200 |
-Data collection
Diffraction | Mean temperature: 105 K | ||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 21, 2011 | ||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.24→51.3 Å / Num. obs: 62550 / % possible obs: 99 % / Redundancy: 3.9 % / Biso Wilson estimate: 49.16 Å2 / Rmerge(I) obs: 0.069 / Net I/σ(I): 6.7 | ||||||||||||
Reflection shell | Resolution: 2.24→2.36 Å / Redundancy: 4.1 % / Mean I/σ(I) obs: 1.5 / % possible all: 99 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Displacement parameters | Biso mean: 55.14 Å2
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Refine analyze | Luzzati coordinate error obs: 0.276 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.24→49.38 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.24→2.3 Å / Total num. of bins used: 20
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