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Open data
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Basic information
Entry | Database: PDB / ID: 4orc | ||||||
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Title | Crystal structure of mammalian calcineurin | ||||||
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![]() | HYDROLASE/METAL BINDING PROTEIN / Calmodulin-binding / HYDROLASE-METAL BINDING PROTEIN complex | ||||||
Function / homology | ![]() calcium-dependent protein serine/threonine phosphatase regulator activity / negative regulation of calcium ion import across plasma membrane / calcium-dependent protein serine/threonine phosphatase activity / negative regulation of signaling / protein serine/threonine phosphatase complex / locomotion involved in locomotory behavior / positive regulation of calcium ion import across plasma membrane / calmodulin-dependent protein phosphatase activity / positive regulation of lysosome organization / calcineurin complex ...calcium-dependent protein serine/threonine phosphatase regulator activity / negative regulation of calcium ion import across plasma membrane / calcium-dependent protein serine/threonine phosphatase activity / negative regulation of signaling / protein serine/threonine phosphatase complex / locomotion involved in locomotory behavior / positive regulation of calcium ion import across plasma membrane / calmodulin-dependent protein phosphatase activity / positive regulation of lysosome organization / calcineurin complex / calcineurin-mediated signaling / negative regulation of T cell mediated cytotoxicity / ROBO receptors bind AKAP5 / lymphangiogenesis / lung epithelial cell differentiation / calcineurin-NFAT signaling cascade / regulation of synaptic vesicle cycle / positive regulation of calcineurin-NFAT signaling cascade / calcium-ion regulated exocytosis / myelination in peripheral nervous system / protein phosphatase 2B binding / axon extension / regulation of postsynaptic neurotransmitter receptor internalization / parallel fiber to Purkinje cell synapse / regulation of synaptic vesicle endocytosis / cyclosporin A binding / myosin phosphatase activity / CLEC7A (Dectin-1) induces NFAT activation / branching involved in blood vessel morphogenesis / postsynaptic modulation of chemical synaptic transmission / protein serine/threonine phosphatase activity / protein-serine/threonine phosphatase / T cell homeostasis / positive regulation of insulin secretion involved in cellular response to glucose stimulus / regulation of insulin secretion / Calcineurin activates NFAT / T cell differentiation / DARPP-32 events / Activation of BAD and translocation to mitochondria / epithelial to mesenchymal transition / phosphatase binding / skeletal muscle fiber development / T cell proliferation / dephosphorylation / T-tubule / T cell activation / hippocampal mossy fiber to CA3 synapse / FCERI mediated Ca+2 mobilization / protein dephosphorylation / learning / response to cytokine / Schaffer collateral - CA1 synapse / regulation of synaptic plasticity / T cell mediated cytotoxicity / memory / sarcolemma / Z disc / positive regulation of protein localization to nucleus / protein import into nucleus / Ca2+ pathway / heart development / postsynapse / protein dimerization activity / calmodulin binding / protein domain specific binding / protein phosphorylation / glutamatergic synapse / calcium ion binding / positive regulation of DNA-templated transcription / enzyme binding / signal transduction / positive regulation of transcription by RNA polymerase II / nucleoplasm / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ma, L. / Li, S.J. / Wang, J. / Wu, J.W. / Wang, Z.X. | ||||||
![]() | ![]() Title: Cooperative autoinhibition and multi-level activation mechanisms of calcineurin Authors: Li, S.J. / Ma, L. / Wang, J. / Lu, C. / Wang, J. / Wu, J.W. / Wang, Z.X. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 234.8 KB | Display | ![]() |
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PDB format | ![]() | 187.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 459.6 KB | Display | ![]() |
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Full document | ![]() | 465.5 KB | Display | |
Data in XML | ![]() | 20.7 KB | Display | |
Data in CIF | ![]() | 28.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4or9C ![]() 4oraC ![]() 4orbC ![]() 1auiS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein , 2 types, 2 molecules AB
#1: Protein | Mass: 61264.473 Da / Num. of mol.: 1 / Fragment: catalytic subunit Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: P16298, protein-serine/threonine phosphatase |
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#2: Protein | Mass: 19322.904 Da / Num. of mol.: 1 / Fragment: regulatory subunit Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
-Non-polymers , 5 types, 48 molecules ![](data/chem/img/ZN.gif)
![](data/chem/img/FE.gif)
![](data/chem/img/PO4.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/FE.gif)
![](data/chem/img/PO4.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | ChemComp-ZN / | ||
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#4: Chemical | ChemComp-FE / | ||
#5: Chemical | ChemComp-PO4 / | ||
#6: Chemical | ChemComp-CA / #7: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.07 Å3/Da / Density % sol: 59.96 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 6.1 Details: 100mM MES, pH 6.1, 10% PEG3350, 0.2M CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX-225 / Detector: CCD / Date: Jun 12, 2010 |
Radiation | Monochromator: double crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97916 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→50 Å / Num. all: 28850 / Num. obs: 28655 / % possible obs: 100 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 14.7 % / Biso Wilson estimate: 45.9 Å2 / Rmerge(I) obs: 0.098 / Net I/σ(I): 23.9 |
Reflection shell | Resolution: 2.7→2.75 Å / Redundancy: 13.6 % / Rmerge(I) obs: 0.615 / Mean I/σ(I) obs: 3 / Num. unique all: 1410 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1AUI Resolution: 2.7→39.538 Å / SU ML: 0.37 / σ(F): 1.34 / Phase error: 26.75 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.73 Å / VDW probe radii: 1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 6.932 Å2 / ksol: 0.336 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.7→39.538 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 10
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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