regulation of RNA metabolic process / negative regulation of viral genome replication / RNA nuclease activity / mRNA processing / defense response to virus / protein kinase activity / RNA binding / ATP binding / metal ion binding Similarity search - Function
RNase L, RNase domain / KEN domain / KEN domain / KEN domain superfamily / Ribonuclease 2-5A / KEN domain profile. / domain in protein kinases, N-glycanases and other nuclear proteins / de novo design (two linked rop proteins) / Ankyrin repeat / Ankyrin repeat-containing domain ...RNase L, RNase domain / KEN domain / KEN domain / KEN domain superfamily / Ribonuclease 2-5A / KEN domain profile. / domain in protein kinases, N-glycanases and other nuclear proteins / de novo design (two linked rop proteins) / Ankyrin repeat / Ankyrin repeat-containing domain / Ankyrin repeats (many copies) / Ankyrin repeat / Ankyrin repeats (3 copies) / Ankyrin repeat profile. / Ankyrin repeat region circular profile. / ankyrin repeats / Ankyrin repeat / Ankyrin repeat-containing domain superfamily / Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat / Alpha Horseshoe / Transferase(Phosphotransferase) domain 1 / Transferase(Phosphotransferase); domain 1 / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily / Up-down Bundle / Orthogonal Bundle / Mainly Alpha Similarity search - Domain/homology
Mass: 1165.593 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C30H40N15O25P5
-
Experimental details
-
Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
-
Sample preparation
Crystal
Density Matthews: 2.76 Å3/Da / Density % sol: 55.47 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 5 mM DTT, 18% PEG2000, 100 mM NaCl, and 100 mM SPG buffer, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution: 3.27→49.7 Å / Cor.coef. Fo:Fc: 0.909 / Cor.coef. Fo:Fc free: 0.867 / SU B: 17.906 / SU ML: 0.328 / Cross valid method: THROUGHOUT / ESU R Free: 0.138 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.28851
1587
3.1 %
RANDOM
Rwork
0.23048
-
-
-
all
0.23225
52702
-
-
obs
0.23225
49593
94.1 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK