Entry Database : PDB / ID : 4nc3 Structure visualization Downloads & linksTitle Crystal structure of the 5-HT2B receptor solved using serial femtosecond crystallography in lipidic cubic phase. ComponentsChimera protein of human 5-hydroxytryptamine receptor 2B and E. Coli soluble cytochrome b562 Details Keywords SIGNALING PROTEIN / ELECTRON TRANSPORT / Serial femtosecond crystallography / human 5HT2B receptor / ergotamine / novel protein engineering / GPCR Network / Membrane protein / lipidic cubic phase / PSI-Biology / free electron laser / Structural Genomics / GPCR / membraneFunction / homology Function and homology informationFunction Domain/homology Component
behavior / intestine smooth muscle contraction / Gq/11-coupled serotonin receptor activity / phospholipase C-activating serotonin receptor signaling pathway / positive regulation of phosphatidylinositol biosynthetic process / G protein-coupled serotonin receptor signaling pathway / G protein-coupled serotonin receptor complex / protein kinase C signaling / regulation of behavior / Serotonin receptors ... behavior / intestine smooth muscle contraction / Gq/11-coupled serotonin receptor activity / phospholipase C-activating serotonin receptor signaling pathway / positive regulation of phosphatidylinositol biosynthetic process / G protein-coupled serotonin receptor signaling pathway / G protein-coupled serotonin receptor complex / protein kinase C signaling / regulation of behavior / Serotonin receptors / embryonic morphogenesis / cellular response to temperature stimulus / serotonin binding / vasoconstriction / G protein-coupled serotonin receptor activity / : / G protein-coupled receptor internalization / cardiac muscle hypertrophy / neural crest cell differentiation / neural crest cell migration / neurotransmitter receptor activity / cGMP-mediated signaling / G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger / : / positive regulation of cell division / G-protein alpha-subunit binding / heart morphogenesis / release of sequestered calcium ion into cytosol / positive regulation of endothelial cell proliferation / ERK1 and ERK2 cascade / phosphatidylinositol 3-kinase/protein kinase B signal transduction / GTPase activator activity / positive regulation of nitric-oxide synthase activity / positive regulation of cytokine production / calcium-mediated signaling / electron transport chain / positive regulation of MAP kinase activity / intracellular calcium ion homeostasis / phospholipase C-activating G protein-coupled receptor signaling pathway / G alpha (q) signalling events / chemical synaptic transmission / positive regulation of canonical NF-kappaB signal transduction / periplasmic space / electron transfer activity / positive regulation of ERK1 and ERK2 cascade / response to xenobiotic stimulus / iron ion binding / G protein-coupled receptor signaling pathway / phosphorylation / synapse / dendrite / heme binding / positive regulation of cell population proliferation / negative regulation of apoptotic process / nucleoplasm / plasma membrane / cytoplasm Similarity search - Function 5-Hydroxytryptamine 2B receptor / 5-hydroxytryptamine receptor family / Cytochrome c/b562 / Rhopdopsin 7-helix transmembrane proteins / Rhodopsin 7-helix transmembrane proteins / Cytochrome b562 / Cytochrome b562 / Cytochrome c/b562 / Serpentine type 7TM GPCR chemoreceptor Srsx / Four Helix Bundle (Hemerythrin (Met), subunit A) ... 5-Hydroxytryptamine 2B receptor / 5-hydroxytryptamine receptor family / Cytochrome c/b562 / Rhopdopsin 7-helix transmembrane proteins / Rhodopsin 7-helix transmembrane proteins / Cytochrome b562 / Cytochrome b562 / Cytochrome c/b562 / Serpentine type 7TM GPCR chemoreceptor Srsx / Four Helix Bundle (Hemerythrin (Met), subunit A) / G-protein coupled receptors family 1 signature. / G protein-coupled receptor, rhodopsin-like / GPCR, rhodopsin-like, 7TM / G-protein coupled receptors family 1 profile. / 7 transmembrane receptor (rhodopsin family) / Up-down Bundle / Mainly Alpha Similarity search - Domain/homology CHOLESTEROL / DIACYL GLYCEROL / Ergotamine / OLEIC ACID / (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate / DI(HYDROXYETHYL)ETHER / PALMITIC ACID / Soluble cytochrome b562 / 5-hydroxytryptamine receptor 2B Similarity search - ComponentBiological species Homo sapiens (human)Escherichia coli (E. coli)Method X-RAY DIFFRACTION / FREE ELECTRON LASER / MOLECULAR REPLACEMENT / Resolution : 2.8 Å DetailsAuthors Liu, W. / Wacker, D. / Gati, C. / Han, G.W. / James, D. / Wang, D. / Nelson, G. / Weierstall, U. / Katritch, V. / Barty, A. ...Liu, W. / Wacker, D. / Gati, C. / Han, G.W. / James, D. / Wang, D. / Nelson, G. / Weierstall, U. / Katritch, V. / Barty, A. / Zatsepin, N.A. / Li, D. / Messerschmidt, M. / Boutet, S. / Williams, G.J. / Koglin, J.E. / Seibert, M.M. / Wang, C. / Shah, S.T.A. / Basu, S. / Fromme, R. / Kupitz, C. / Rendek, K.N. / Grotjohann, I. / Fromme, P. / Kirian, R.A. / Beyerlein, K.R. / White, T.A. / Chapman, H.N. / Caffrey, M. / Spence, J.C.H. / Stevens, R.C. / Cherezov, V. / GPCR Network (GPCR) CitationJournal : Science / Year : 2013Title : Serial femtosecond crystallography of G protein-coupled receptors.Authors: Liu, W. / Wacker, D. / Gati, C. / Han, G.W. / James, D. / Wang, D. / Nelson, G. / Weierstall, U. / Katritch, V. / Barty, A. / Zatsepin, N.A. / Li, D. / Messerschmidt, M. / Boutet, S. / ... Authors : Liu, W. / Wacker, D. / Gati, C. / Han, G.W. / James, D. / Wang, D. / Nelson, G. / Weierstall, U. / Katritch, V. / Barty, A. / Zatsepin, N.A. / Li, D. / Messerschmidt, M. / Boutet, S. / Williams, G.J. / Koglin, J.E. / Seibert, M.M. / Wang, C. / Shah, S.T. / Basu, S. / Fromme, R. / Kupitz, C. / Rendek, K.N. / Grotjohann, I. / Fromme, P. / Kirian, R.A. / Beyerlein, K.R. / White, T.A. / Chapman, H.N. / Caffrey, M. / Spence, J.C. / Stevens, R.C. / Cherezov, V. History Deposition Oct 23, 2013 Deposition site : RCSB / Processing site : RCSBRevision 1.0 Dec 18, 2013 Provider : repository / Type : Initial releaseRevision 1.1 Jan 22, 2014 Group : Database referencesRevision 1.2 Jun 7, 2017 Group : Database references / Structure summaryRevision 1.3 Feb 14, 2018 Group : Data collection / Category : diffrn_sourceItem : _diffrn_source.pdbx_synchrotron_beamline / _diffrn_source.pdbx_synchrotron_siteRevision 1.4 Aug 16, 2023 Group : Data collection / Database references / Derived calculationsCategory : chem_comp_atom / chem_comp_bond ... chem_comp_atom / chem_comp_bond / database_2 / pdbx_related_exp_data_set / struct_conn / struct_ref_seq_dif / struct_site Item : _database_2.pdbx_DOI / _database_2.pdbx_database_accession ... _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_leaving_atom_flag / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id Revision 1.5 Sep 20, 2023 Group : Refinement description / Category : pdbx_initial_refinement_model
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