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- PDB-4n0u: Ternary complex between Neonatal Fc receptor, serum albumin and Fc -
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Open data
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Basic information
Entry | Database: PDB / ID: 4n0u | |||||||||
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Title | Ternary complex between Neonatal Fc receptor, serum albumin and Fc | |||||||||
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![]() | DNA BINDING PROTEIN / alpha/beta | |||||||||
Function / homology | ![]() IgG immunoglobulin transcytosis in epithelial cells mediated by FcRn immunoglobulin receptor / Fc-gamma receptor I complex binding / cellular response to calcium ion starvation / exogenous protein binding / Ciprofloxacin ADME / complement-dependent cytotoxicity / IgG immunoglobulin complex / enterobactin binding / Heme biosynthesis / antibody-dependent cellular cytotoxicity ...IgG immunoglobulin transcytosis in epithelial cells mediated by FcRn immunoglobulin receptor / Fc-gamma receptor I complex binding / cellular response to calcium ion starvation / exogenous protein binding / Ciprofloxacin ADME / complement-dependent cytotoxicity / IgG immunoglobulin complex / enterobactin binding / Heme biosynthesis / antibody-dependent cellular cytotoxicity / IgG binding / HDL remodeling / negative regulation of mitochondrial depolarization / Prednisone ADME / Heme degradation / Classical antibody-mediated complement activation / Initial triggering of complement / Aspirin ADME / antioxidant activity / FCGR activation / toxic substance binding / Role of phospholipids in phagocytosis / beta-2-microglobulin binding / Scavenging of heme from plasma / immunoglobulin complex, circulating / immunoglobulin receptor binding / Recycling of bile acids and salts / cellular response to starvation / FCGR3A-mediated IL10 synthesis / complement activation, classical pathway / positive regulation of ferrous iron binding / positive regulation of transferrin receptor binding / platelet alpha granule lumen / positive regulation of receptor binding / early endosome lumen / Nef mediated downregulation of MHC class I complex cell surface expression / negative regulation of receptor binding / DAP12 interactions / Regulation of Complement cascade / antigen binding / fatty acid binding / cellular response to iron ion / Endosomal/Vacuolar pathway / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / FCGR3A-mediated phagocytosis / Post-translational protein phosphorylation / B cell receptor signaling pathway / cellular response to iron(III) ion / antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent / negative regulation of forebrain neuron differentiation / regulation of erythrocyte differentiation / peptide antigen assembly with MHC class I protein complex / ER to Golgi transport vesicle membrane / regulation of iron ion transport / response to molecule of bacterial origin / MHC class I peptide loading complex / HFE-transferrin receptor complex / T cell mediated cytotoxicity / Cytoprotection by HMOX1 / antigen processing and presentation of endogenous peptide antigen via MHC class I / positive regulation of T cell cytokine production / Regulation of actin dynamics for phagocytic cup formation / MHC class I protein complex / multicellular organismal-level iron ion homeostasis / negative regulation of neurogenesis / positive regulation of receptor-mediated endocytosis / peptide antigen assembly with MHC class II protein complex / MHC class II protein complex / cellular response to nicotine / positive regulation of T cell mediated cytotoxicity / specific granule lumen / recycling endosome membrane / phagocytic vesicle membrane / peptide antigen binding / positive regulation of cellular senescence / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / negative regulation of epithelial cell proliferation / antigen processing and presentation of exogenous peptide antigen via MHC class II / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / Interferon gamma signaling / positive regulation of immune response / Modulation by Mtb of host immune system / positive regulation of T cell activation / sensory perception of smell / negative regulation of neuron projection development / pyridoxal phosphate binding / positive regulation of protein binding / tertiary granule lumen / Platelet degranulation / DAP12 signaling / MHC class II protein complex binding / late endosome membrane / protein-folding chaperone binding / iron ion transport / antibacterial humoral response / ER-Phagosome pathway / early endosome membrane / T cell differentiation in thymus / protein refolding / Interleukin-4 and Interleukin-13 signaling Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Oganesyan, V. / Wu, H. / Dall'Acqua, W.F. | |||||||||
![]() | ![]() Title: Structural Insights into Neonatal Fc Receptor-based Recycling Mechanisms. Authors: Oganesyan, V. / Damschroder, M.M. / Cook, K.E. / Li, Q. / Gao, C. / Wu, H. / Dall'acqua, W.F. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 364.6 KB | Display | ![]() |
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PDB format | ![]() | 290.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 29433.115 Da / Num. of mol.: 1 / Fragment: FcRn, alpha chain, ecd, unp residue 27-290 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Protein | Mass: 11748.160 Da / Num. of mol.: 1 / Fragment: beta-2 microglobulin, unp residues 21-119 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
#3: Protein | Mass: 66385.055 Da / Num. of mol.: 1 / Fragment: serum albumin, unp residue 27-609 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: ALB, GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341 Production host: ![]() ![]() |
#4: Protein | Mass: 23765.738 Da / Num. of mol.: 1 / Fragment: IgG1 Fc, unp reisdues 119-327 / Mutation: M252Y, S254T, T256E Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
#5: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.23 Å3/Da / Density % sol: 61.86 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion / pH: 5.2 Details: 4% Tacsimate, pH 4.0, 12% PEG 3350, VAPOR DIFFUSION, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Nov 14, 2012 / Details: Compound Parabolic Refractive X-ray Lenses |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3.8→40 Å / Num. all: 17596 / Num. obs: 17420 / % possible obs: 99.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 143.837 Å2
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Refinement step | Cycle: LAST / Resolution: 3.8→40 Å
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