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Open data
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Basic information
Entry | Database: PDB / ID: 4moe | ||||||
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Title | Pyranose 2-oxidase H450G mutant with 3-fluorinated glucose | ||||||
![]() | Pyranose 2-oxidase | ||||||
![]() | OXIDOREDUCTASE / GMC OXIDOREDUCTASE / PHBH FOLD / HOMOTETRAMER / FAD-BINDING / SUBSTRATE COMPLEX / FLAVINYLATION / INTRACELLULAR | ||||||
Function / homology | ![]() pyranose oxidase / pyranose oxidase activity / flavin adenine dinucleotide binding / periplasmic space Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Tan, T.C. / Spadiut, O. / Gandini, R. / Haltrich, D. / Divne, C. | ||||||
![]() | ![]() Title: Structural Basis for Binding of Fluorinated Glucose and Galactose to Trametes multicolor Pyranose 2-Oxidase Variants with Improved Galactose Conversion. Authors: Tan, T.C. / Spadiut, O. / Gandini, R. / Haltrich, D. / Divne, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 902.7 KB | Display | ![]() |
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PDB format | ![]() | 758.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.2 MB | Display | ![]() |
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Full document | ![]() | 2.3 MB | Display | |
Data in XML | ![]() | 95.5 KB | Display | |
Data in CIF | ![]() | 134.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4mofC ![]() 4mogC ![]() 4mohC ![]() 4moiC ![]() 4mojC ![]() 4mokC ![]() 4molC ![]() 4momC ![]() 4mooC ![]() 4mopC ![]() 4moqC ![]() 4morC ![]() 4mosC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein / Sugars , 2 types, 8 molecules ABCD![](data/chem/img/G3F.gif)
![](data/chem/img/G3F.gif)
#1: Protein | Mass: 70472.961 Da / Num. of mol.: 4 / Mutation: H450G Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: Sugar | ChemComp-G3F / |
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-Non-polymers , 4 types, 1129 molecules ![](data/chem/img/FDA.gif)
![](data/chem/img/12P.gif)
![](data/chem/img/MES.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/12P.gif)
![](data/chem/img/MES.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-FDA / #4: Chemical | ChemComp-12P / #5: Chemical | ChemComp-MES / #6: Water | ChemComp-HOH / | |
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-Details
Sequence details | THIS IS A CLONING ARTIFACT |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.51 Å3/Da / Density % sol: 51.01 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5.2 Details: 0.1M MES, 50mM MgCl2, 10% (w/v) monomethylether PEG 2000 , pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Apr 4, 2009 / Details: Multilayer mirror |
Radiation | Monochromator: Bent Si(111) crystal, horisontally focusing / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0379 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→57.19 Å / Num. all: 214367 / Num. obs: 214367 / % possible obs: 97.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.2 % / Net I/σ(I): 12.4 |
Reflection shell | Resolution: 1.9→2 Å / Redundancy: 3.2 % / Mean I/σ(I) obs: 2.5 / % possible all: 98.4 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 25.91 Å2
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Refinement step | Cycle: LAST / Resolution: 2→50 Å
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Refine LS restraints |
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