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- PDB-4mm4: Crystal structure of LeuBAT (delta13 mutant) in complex with paro... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4mm4 | ||||||
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Title | Crystal structure of LeuBAT (delta13 mutant) in complex with paroxetine | ||||||
![]() | Transporter | ||||||
![]() | TRANSPORT PROTEIN / LeuT fold / transporter | ||||||
Function / homology | Sodium:neurotransmitter symporter / Sodium:neurotransmitter symporter superfamily / Sodium:neurotransmitter symporter family / Sodium:neurotransmitter symporter family profile. / sodium ion transmembrane transport / plasma membrane / Paroxetine / Na(+):neurotransmitter symporter (Snf family)![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Wang, H. / Gouaux, E. | ||||||
![]() | ![]() Title: Structural basis for action by diverse antidepressants on biogenic amine transporters. Authors: Wang, H. / Goehring, A. / Wang, K.H. / Penmatsa, A. / Ressler, R. / Gouaux, E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 205.4 KB | Display | ![]() |
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PDB format | ![]() | 165.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4mm5C ![]() 4mm6C ![]() 4mm7C ![]() 4mm8C ![]() 4mm9C ![]() 4mmaC ![]() 4mmbC ![]() 4mmcC ![]() 4mmdC ![]() 4mmeC ![]() 4mmfC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 |
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Unit cell |
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Components
#1: Protein | Mass: 57988.266 Da / Num. of mol.: 2 Mutation: N21Y, G24D, I106S, T254S, S256G, A261V, I262L, Y265F, E290S, I359G, P362G, G408T, T409G Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-NA / #3: Chemical | #4: Chemical | ChemComp-CL / | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.97 Å3/Da / Density % sol: 58.52 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion / pH: 9.4 Details: 100 mM glycine (pH9.4), 0.1M Li2SO4, 29%-31% PEG400, VAPOR DIFFUSION, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Feb 14, 2013 |
Radiation | Monochromator: Cryogenically-cooled single crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 2.886→40 Å / Num. all: 30590 / Num. obs: 30223 / % possible obs: 98.8 % / Observed criterion σ(I): -3 / Redundancy: 3.7 % / Rmerge(I) obs: 0.114 / Net I/σ(I): 11 |
Reflection shell | Resolution: 2.886→3 Å / Rmerge(I) obs: 0.73 / Mean I/σ(I) obs: 1.2 / % possible all: 95 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.886→37.313 Å
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Refine LS restraints |
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LS refinement shell |
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