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Yorodumi- PDB-4m1c: Crystal Structure Analysis of Fab-Bound Human Insulin Degrading E... -
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-Basic information
Entry | Database: PDB / ID: 4m1c | ||||||
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Title | Crystal Structure Analysis of Fab-Bound Human Insulin Degrading Enzyme (IDE) in Complex with Amyloid-Beta (1-40) | ||||||
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Keywords | HYDROLASE / Zinc metalloprotease | ||||||
Function / homology | Function and homology information insulysin / ubiquitin recycling / insulin catabolic process / insulin metabolic process / amyloid-beta clearance by cellular catabolic process / hormone catabolic process / bradykinin catabolic process / regulation of epidermal growth factor-activated receptor activity / signaling receptor activator activity / cytosolic mRNA polyadenylation ...insulysin / ubiquitin recycling / insulin catabolic process / insulin metabolic process / amyloid-beta clearance by cellular catabolic process / hormone catabolic process / bradykinin catabolic process / regulation of epidermal growth factor-activated receptor activity / signaling receptor activator activity / cytosolic mRNA polyadenylation / collateral sprouting in absence of injury / microglia development / regulation of synapse structure or activity / Formyl peptide receptors bind formyl peptides and many other ligands / axo-dendritic transport / synaptic assembly at neuromuscular junction / smooth endoplasmic reticulum calcium ion homeostasis / axon midline choice point recognition / astrocyte activation involved in immune response / regulation of spontaneous synaptic transmission / regulation of Wnt signaling pathway / mating behavior / positive regulation of amyloid fibril formation / ubiquitin-modified protein reader activity / ciliary rootlet / Lysosome Vesicle Biogenesis / insulin binding / PTB domain binding / Golgi-associated vesicle / neuron remodeling / regulation of aerobic respiration / Insertion of tail-anchored proteins into the endoplasmic reticulum membrane / : / Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models / presynaptic active zone / nuclear envelope lumen / peptide catabolic process / modulation of excitatory postsynaptic potential / suckling behavior / COPII-coated ER to Golgi transport vesicle / dendrite development / amyloid-beta clearance / smooth endoplasmic reticulum / regulation of NMDA receptor activity / TRAF6 mediated NF-kB activation / negative regulation of long-term synaptic potentiation / peroxisomal matrix / Advanced glycosylation endproduct receptor signaling / neuromuscular process controlling balance / regulation of presynapse assembly / The NLRP3 inflammasome / intracellular copper ion homeostasis / transition metal ion binding / regulation of multicellular organism growth / negative regulation of neuron differentiation / ECM proteoglycans / spindle midzone / positive regulation of T cell migration / Purinergic signaling in leishmaniasis infection / positive regulation of calcium-mediated signaling / amyloid-beta metabolic process / forebrain development / regulation of peptidyl-tyrosine phosphorylation / positive regulation of chemokine production / clathrin-coated pit / Notch signaling pathway / cholesterol metabolic process / Insulin receptor recycling / positive regulation of G2/M transition of mitotic cell cycle / positive regulation of protein metabolic process / ionotropic glutamate receptor signaling pathway / neuron projection maintenance / positive regulation of glycolytic process / extracellular matrix organization / proteolysis involved in protein catabolic process / positive regulation of mitotic cell cycle / response to interleukin-1 / axonogenesis / adult locomotory behavior / trans-Golgi network membrane / dendritic shaft / locomotory behavior / platelet alpha granule lumen / positive regulation of peptidyl-threonine phosphorylation / learning / positive regulation of interleukin-1 beta production / central nervous system development / positive regulation of long-term synaptic potentiation / astrocyte activation / endosome lumen / Post-translational protein phosphorylation / Peroxisomal protein import / synapse organization / peptide binding / microglial cell activation / regulation of long-term neuronal synaptic plasticity / positive regulation of JNK cascade / protein catabolic process / TAK1-dependent IKK and NF-kappa-B activation / neuromuscular junction Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.5007 Å | ||||||
Authors | McCord, L.M. / Liang, W. / Farcasanu, M. / Scherpelz, K. / Meredith, S.C. / Koide, S. / Tang, W.J. | ||||||
Citation | Journal: To be Published Title: Crystal Structure Analysis of Fab-Bound Human Insulin Degrading Enzyme (IDE) in Complex with Amyloid-Beta (1-40) Authors: McCord, L.A. / Liang, W. / Farcasanu, M. / Scherpelz, K. / Meredith, S.C. / Koide, S. / Tang, W.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4m1c.cif.gz | 1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb4m1c.ent.gz | 889.5 KB | Display | PDB format |
PDBx/mmJSON format | 4m1c.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m1/4m1c ftp://data.pdbj.org/pub/pdb/validation_reports/m1/4m1c | HTTPS FTP |
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-Related structure data
Related structure data | 4iofS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
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-Components
#1: Protein | Mass: 114560.578 Da / Num. of mol.: 2 / Mutation: E111Q Source method: isolated from a genetically manipulated source Details: Cysteine-Free / Source: (gene. exp.) Homo sapiens (human) / Gene: IDE, Insulin Degrading Enzyme / Production host: Escherichia coli (E. coli) / References: UniProt: P14735, insulysin #2: Antibody | Mass: 28201.670 Da / Num. of mol.: 2 / Source method: obtained synthetically Details: Specific antigen-binding fragment (Fab, light chain) synthetically engineered to target Human Insulin Degrading Enzyme Source: (synth.) Homo sapiens (human) #3: Antibody | Mass: 25982.098 Da / Num. of mol.: 2 / Source method: obtained synthetically Details: Specific antigen-binding fragment (Fab, heavy chain) synthetically engineered to target Human Insulin Degrading Enzyme Source: (synth.) Homo sapiens (human) #4: Protein/peptide | Mass: 4335.852 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: Amyloid-Beta (1-40) / Source: (synth.) Homo sapiens (human) / References: UniProt: P05067 #5: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.04 Å3/Da / Density % sol: 39.65 % |
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Crystal grow | Temperature: 291.15 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 0.1M Sodium cacodylate, pH6.5, 0.2M MgCl2, 10% PEG-3000, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
-Data collection
Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.9792 Å |
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Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jun 10, 2013 |
Radiation | Monochromator: MAR CCD / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 |
Reflection | Resolution: 3.5→49.1 Å / Num. all: 35563 / Num. obs: 35563 / % possible obs: 96.6 % / Redundancy: 5.5 % / Biso Wilson estimate: 52.45 Å2 / Rmerge(I) obs: 0.159 |
Reflection shell | Resolution: 3.5→3.56 Å / Redundancy: 5.6 % / Rmerge(I) obs: 0.68 / % possible all: 96.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 4IOF Resolution: 3.5007→49.064 Å / SU ML: 0.45 / σ(F): 1.34 / Phase error: 26.98 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.5007→49.064 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 13.9556 Å / Origin y: -8.5682 Å / Origin z: -98.8944 Å
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Refinement TLS group | Selection details: all |