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- PDB-4lx4: Crystal Structure Determination of Pseudomonas stutzeri endogluca... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4lx4 | ||||||
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Title | Crystal Structure Determination of Pseudomonas stutzeri endoglucanase Cel5A using a Twinned Data Set | ||||||
![]() | Endoglucanase(Endo-1,4-beta-glucanase)protein | ||||||
![]() | HYDROLASE / GLYCOSYL HYDROLASE FAMILY 5 / CELLULASE / TIM BARREL / BETA-1 / 4-ENDOGLUCANASE | ||||||
Function / homology | ![]() hydrolase activity, hydrolyzing O-glycosyl compounds / carbohydrate metabolic process Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Dutoit, R. / Delsaute, M. / Berlemont, R. / Van Elder, D. / Galleni, M. / Bauvois, C. | ||||||
![]() | ![]() Title: Crystal structure determination of Pseudomonas stutzeri A1501 endoglucanase Cel5A: the search for a molecular basis for glycosynthesis in GH5_5 enzymes. Authors: Dutoit, R. / Delsaute, M. / Collet, L. / Vander Wauven, C. / Van Elder, D. / Berlemont, R. / Richel, A. / Galleni, M. / Bauvois, C. #1: Journal: ISME J / Year: 2009 Title: Insights into bacterial cellulose biosynthesis by functional metagenomics on Antarctic soil samples. Authors: Berlemont, R. / Delsaute, M. / Pipers, D. / D'Amico, S. / Feller, G. / Galleni, M. / Power, P. #2: Journal: Proc.Natl.Acad.Sci.USA / Year: 2008 Title: Nitrogen fixation island and rhizosphere competence traits in the genome of root-associated Pseudomonas stutzeri A1501. Authors: Yan, Y. / Yang, J. / Dou, Y. / Chen, M. / Ping, S. / Peng, J. / Lu, W. / Zhang, W. / Yao, Z. / Li, H. / Liu, W. / He, S. / Geng, L. / Zhang, X. / Yang, F. / Yu, H. / Zhan, Y. / Li, D. / Lin, ...Authors: Yan, Y. / Yang, J. / Dou, Y. / Chen, M. / Ping, S. / Peng, J. / Lu, W. / Zhang, W. / Yao, Z. / Li, H. / Liu, W. / He, S. / Geng, L. / Zhang, X. / Yang, F. / Yu, H. / Zhan, Y. / Li, D. / Lin, Z. / Wang, Y. / Elmerich, C. / Lin, M. / Jin, Q. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 478.8 KB | Display | ![]() |
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PDB format | ![]() | 404.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 477.6 KB | Display | ![]() |
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Full document | ![]() | 499.7 KB | Display | |
Data in XML | ![]() | 59.7 KB | Display | |
Data in CIF | ![]() | 87.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4ee9S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 37216.852 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-TRS / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.05 Å3/Da / Density % sol: 40.04 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 7 Details: Ps_Cel5A (27 microM) in 50 mM sodium phosphate pH 7.0, was mixed 1:1 with well buffer (100 mM tris pH 5.9 with 22.5 % v/v polyethylene glycol 600) using the hanging drop method with 500 uL ...Details: Ps_Cel5A (27 microM) in 50 mM sodium phosphate pH 7.0, was mixed 1:1 with well buffer (100 mM tris pH 5.9 with 22.5 % v/v polyethylene glycol 600) using the hanging drop method with 500 uL well buffer in the well of the crystallization tray. Crystals obtained by this method were soaked 1 hour in 100 mM Tris pH 5.9 with 30% v/v polyethylene glycol 600., VAPOR DIFFUSION, HANGING DROP, temperature 292K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: May 18, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979639 Å / Relative weight: 1 |
Reflection | Resolution: 1.556→48.279 Å / Num. all: 157788 / Num. obs: 157658 / % possible obs: 91.6 % / Observed criterion σ(I): -3 / Redundancy: 7.1 % / Biso Wilson estimate: 20.1 Å2 / Rmerge(I) obs: 0.076 / Rsym value: 0.07 / Net I/σ(I): 17.9 |
Reflection shell | Resolution: 1.56→1.6 Å / Redundancy: 7.07 % / Rmerge(I) obs: 0.339 / Mean I/σ(I) obs: 5.9 / Num. unique all: 12421 / Rsym value: 0.314 / % possible all: 97.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 4EE9 Resolution: 1.556→48.279 Å / Isotropic thermal model: Isotropic / σ(F): 1.36 / Phase error: 27.74 / Stereochemistry target values: TWIN_LSQ_F
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 12.4 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.556→48.279 Å
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Refine LS restraints |
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LS refinement shell |
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