- PDB-4lso: Crystal structure of the soluble domain of a Type IV secretion sy... -
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ID or keywords:
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Basic information
Entry
Database: PDB / ID: 4lso
Title
Crystal structure of the soluble domain of a Type IV secretion system protein VirB8 from Bartonella quintana Toulouse
Components
Type IV secretion system protein virB8
Keywords
PROTEIN TRANSPORT / Structural Genomics / NIAID / National Institute of Allergy and Infectious Diseases / Seattle Structural Genomics Center for Infectious Disease / SSGCID / dimer-monomer analysis / type IV secretion system / innate response / human pathogen / host specific protein / cell adhesion
Function / homology
Function and homology information
protein secretion by the type IV secretion system / : / membrane => GO:0016020 / plasma membrane Similarity search - Function
VirB8 protein / Type IV secretion system protein VirB8/PtlE / Bacterial virulence protein VirB8 / VirB8 protein / NTF2-like domain superfamily / Nuclear Transport Factor 2; Chain: A, / Roll / Alpha Beta Similarity search - Domain/homology
Mass: 18.015 Da / Num. of mol.: 162 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 1.94 Å3/Da / Density % sol: 36.56 %
Crystal grow
Temperature: 289 K / Method: vapor diffusion, sitting drop / pH: 5 Details: BaquA.18388.b.B2.PS01732 at 21 mg/mL against JCSG+ screen condition B9, 0.1 M Na citrate pH 5.0, 20% PEG 6000 with 20% ethylene glycol as cryo-protectant, crystal tracking ID 242024b9, ...Details: BaquA.18388.b.B2.PS01732 at 21 mg/mL against JCSG+ screen condition B9, 0.1 M Na citrate pH 5.0, 20% PEG 6000 with 20% ethylene glycol as cryo-protectant, crystal tracking ID 242024b9, unique puck ID fez5-10, VAPOR DIFFUSION, SITTING DROP, temperature 289K