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- PDB-4lqf: Structure of murine IgG2b A2C7-Fab in complex with vaccinia antig... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4lqf | ||||||
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Title | Structure of murine IgG2b A2C7-Fab in complex with vaccinia antigen A33R at the resolution of 2.3 Angstroms | ||||||
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![]() | IMMUNE SYSTEM / IgG domain / antibody-antigen complex / Fv / CH1 / IgG2b / antigen-binding fragment (Fab) / A33R antigen / Papain digest of the mAb / EEV membrane (outer membrane of vaccinia EV form) | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Matho, M.H. / Schlossman, A.M. / Zajonc, D.M. | ||||||
![]() | ![]() Title: Structural and Functional Characterization of Anti-A33 Antibodies Reveal a Potent Cross-Species Orthopoxviruses Neutralizer. Authors: Matho, M.H. / Schlossman, A. / Meng, X. / Benhnia, M.R. / Kaever, T. / Buller, M. / Doronin, K. / Parker, S. / Peters, B. / Crotty, S. / Xiang, Y. / Zajonc, D.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 208.9 KB | Display | ![]() |
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PDB format | ![]() | 167.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4lu5C ![]() 4m1gC ![]() 3k7bS ![]() 4hdiS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 11049.040 Da / Num. of mol.: 1 / Fragment: ECTODOMAIN (UNP RESIDUES 89-185) / Mutation: S89M, L118M, K123A, L140M Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||||
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#2: Antibody | Mass: 23137.764 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Description: Fusion of SP2/0 myeloma cell line with splenocytes Cell: Hybridoma / Production host: ![]() ![]() | ||||
#3: Antibody | Mass: 24234.922 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Description: Fusion of SP2/0 myeloma cell line with splenocytes Cell: Hybridoma / Production host: ![]() ![]() | ||||
#4: Chemical | ChemComp-ZN / #5: Water | ChemComp-HOH / | Sequence details | AUTHORS PROVIDED SEQUENCE DATABASE REFERENCES: ANTI A33 VACCINIA ANTIBODY IGG2B A2C7 HEAVY CHAIN GI ...AUTHORS PROVIDED SEQUENCE DATABASE REFERENCES | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.47 Å3/Da / Density % sol: 50.27 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8 Details: 20% PEG3000, 0.1M imidazole, 0.2M zinc acetate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 29, 2013 Details: Monochromator Side scattering bent cube-root I-beam single crystal, asymmetric cut 4.965 degs, Crystal Type Si(111), Mirrors Rh coated flat mirror |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97945 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→49.76 Å / Num. all: 26224 / Num. obs: 25343 / % possible obs: 96.9 % / Observed criterion σ(I): 2 / Redundancy: 4.8 % / Rmerge(I) obs: 0.095 / Rsym value: 0.095 / Net I/σ(I): 11.3 |
Reflection shell | Resolution: 2.3→2.42 Å / Redundancy: 4.4 % / Rmerge(I) obs: 0.644 / Mean I/σ(I) obs: 2 / Rsym value: 0.644 / % possible all: 90.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: A20G2-Fv domain (reported in same publication), conserved domain of 3E5 IgG3 Fab (extracted from pdb entry 4HDI), one monomer of A33 (extracted from pdb entry 3K7B) Resolution: 2.3→49.76 Å / Cor.coef. Fo:Fc: 0.937 / Cor.coef. Fo:Fc free: 0.916 / SU B: 7.606 / SU ML: 0.101 / Cross valid method: THROUGHOUT / ESU R: 0.07 / ESU R Free: 0.047 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 34.391 Å2
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Refinement step | Cycle: LAST / Resolution: 2.3→49.76 Å
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Refine LS restraints |
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