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- PDB-4le3: Crystal structure of a GH131 beta-glucanase catalytic domain from... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4le3 | ||||||
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Title | Crystal structure of a GH131 beta-glucanase catalytic domain from Podospora anserina | ||||||
![]() | Beta-glucanase | ||||||
![]() | HYDROLASE / glucanase / GH131 | ||||||
Function / homology | ![]() cellulose binding / carbohydrate metabolic process / extracellular region Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Jiang, T. / Chan, H.C. / Huang, C.H. / Ko, T.P. / Huang, T.Y. / Liu, J.R. / Guo, R.T. | ||||||
![]() | ![]() Title: Structures of a GH131 beta-Glucanase Catalytic Domain from Podospora anserina in Complex with Cellotriose Authors: Jiang, T. / Chan, H.C. / Huang, C.H. / Ko, T.P. / Huang, T.Y. / Liu, J.R. / Guo, R.T. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 226.7 KB | Display | ![]() |
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PDB format | ![]() | 181.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 452.9 KB | Display | ![]() |
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Full document | ![]() | 469 KB | Display | |
Data in XML | ![]() | 51.4 KB | Display | |
Data in CIF | ![]() | 77.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 27718.361 Da / Num. of mol.: 4 / Fragment: UNP residues 19-270 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.28 Å3/Da / Density % sol: 46.08 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 0.1M HEPES, 0.7M LiCl, 33% PEG6K, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: May 3, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→25 Å / Num. obs: 86955 / % possible obs: 96.2 % / Redundancy: 3 % / Rmerge(I) obs: 0.069 |
Reflection shell | Resolution: 1.8→1.86 Å / Redundancy: 3.1 % / Rmerge(I) obs: 0.297 / Mean I/σ(I) obs: 4.3 / Num. unique all: 8620 / % possible all: 95.4 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 1.8→25 Å
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LS refinement shell | Resolution: 1.8→1.86 Å /
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