+Open data
-Basic information
Entry | Database: PDB / ID: 4ld8 | ||||||
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Title | Crystal Structure of Dimeric Sudan Virus VP40 | ||||||
Components | Matrix protein VP40 | ||||||
Keywords | VIRAL PROTEIN / viral matrix protein / matrix assembly / viral budding / viral transcription regulation | ||||||
Function / homology | Function and homology information host cell endomembrane system / host cell late endosome membrane / viral budding via host ESCRT complex / structural constituent of virion / ribonucleoprotein complex / host cell plasma membrane / virion membrane / RNA binding / membrane Similarity search - Function | ||||||
Biological species | Sudan ebolavirus | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.83 Å | ||||||
Authors | Bornholdt, Z.A. / Ableson, D.M. / Saphire, E.O. | ||||||
Citation | Journal: Cell(Cambridge,Mass.) / Year: 2013 Title: Structural Rearrangement of Ebola Virus VP40 Begets Multiple Functions in the Virus Life Cycle. Authors: Bornholdt, Z.A. / Noda, T. / Abelson, D.M. / Halfmann, P. / Wood, M.R. / Kawaoka, Y. / Saphire, E.O. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4ld8.cif.gz | 108.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4ld8.ent.gz | 83.2 KB | Display | PDB format |
PDBx/mmJSON format | 4ld8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ld/4ld8 ftp://data.pdbj.org/pub/pdb/validation_reports/ld/4ld8 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 32603.617 Da / Num. of mol.: 1 / Fragment: UNP residues 44-326 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sudan ebolavirus / Gene: VP40 / Production host: Escherichia coli (E. coli) / References: UniProt: Q5XX06 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.12 Å3/Da / Density % sol: 42.05 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 25% PEG400, 0.2 M magnesium chloride, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
-Data collection
Diffraction | Mean temperature: 85 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.97929 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Apr 10, 2010 |
Radiation | Monochromator: Rosenbaum-Rock high-resolution double-crystal monochromator: LN2 cooled first crystal, sagittal focusing 2nd crystal Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97929 Å / Relative weight: 1 |
Reflection | Resolution: 1.83→33.034 Å / Num. all: 23931 / Num. obs: 23880 / % possible obs: 99.7 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 |
Reflection shell | Highest resolution: 1.83 Å |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.83→33.034 Å / SU ML: 0.19 / σ(F): 1.34 / Phase error: 26.75 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.83→33.034 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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