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- PDB-4l1u: Crystal Structure of Human Rtf1 Plus3 Domain in Complex with Spt5... -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 4l1u
TitleCrystal Structure of Human Rtf1 Plus3 Domain in Complex with Spt5 CTR Phosphopeptide
Components
  • RNA polymerase-associated protein RTF1 homolog
  • Transcription elongation factor SPT5
KeywordsTranscription/Peptide / Tutor / Plus3 / Peptide binding protein / Spt5 CTR binding / Transcription / Paf1 complex / Rtf1 / ORF association region / Chromatin / Transcription-Peptide complex
Function / homology
Function and homology information


blastocyst growth / negative regulation of DNA-templated transcription, elongation / Cdc73/Paf1 complex / endodermal cell fate commitment / DSIF complex / regulation of transcription elongation by RNA polymerase II / positive regulation of DNA-templated transcription, elongation / Abortive elongation of HIV-1 transcript in the absence of Tat / transcription elongation-coupled chromatin remodeling / stem cell population maintenance ...blastocyst growth / negative regulation of DNA-templated transcription, elongation / Cdc73/Paf1 complex / endodermal cell fate commitment / DSIF complex / regulation of transcription elongation by RNA polymerase II / positive regulation of DNA-templated transcription, elongation / Abortive elongation of HIV-1 transcript in the absence of Tat / transcription elongation-coupled chromatin remodeling / stem cell population maintenance / RNA Pol II CTD phosphorylation and interaction with CE during HIV infection / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / Formation of the HIV-1 Early Elongation Complex / mRNA Capping / Pausing and recovery of Tat-mediated HIV elongation / Tat-mediated HIV elongation arrest and recovery / positive regulation of macroautophagy / HIV elongation arrest and recovery / Pausing and recovery of HIV elongation / RNA polymerase II transcribes snRNA genes / Tat-mediated elongation of the HIV-1 transcript / Formation of HIV-1 elongation complex containing HIV-1 Tat / Formation of HIV elongation complex in the absence of HIV Tat / RNA Polymerase II Transcription Elongation / Formation of RNA Pol II elongation complex / RNA Polymerase II Pre-transcription Events / TP53 Regulates Transcription of DNA Repair Genes / transcription elongation by RNA polymerase II / Wnt signaling pathway / E3 ubiquitin ligases ubiquitinate target proteins / chromatin organization / single-stranded DNA binding / protein heterodimerization activity / mRNA binding / chromatin binding / nucleolus / negative regulation of transcription by RNA polymerase II / enzyme binding / positive regulation of transcription by RNA polymerase II / RNA binding / nucleoplasm / nucleus
Similarity search - Function
Plus-3 domain / Plus-3 domain / Plus3-like superfamily / Plus-3 domain / Plus3 domain profile. / Short conserved domain in transcriptional regulators. / Spt5, KOW domain repeat 6 / Transcription elongation factor Spt5, eukaryote / Spt5 transcription elongation factor, N-terminal / Spt5, KOW domain repeat 2 ...Plus-3 domain / Plus-3 domain / Plus3-like superfamily / Plus-3 domain / Plus3 domain profile. / Short conserved domain in transcriptional regulators. / Spt5, KOW domain repeat 6 / Transcription elongation factor Spt5, eukaryote / Spt5 transcription elongation factor, N-terminal / Spt5, KOW domain repeat 2 / Spt5, KOW domain repeat 3 / Spt5, KOW domain repeat 5 / Spt5 transcription elongation factor, acidic N-terminal / NGN domain, eukaryotic / Spt5, KOW domain repeat 1 / Spt5, KOW domain repeat 4 / Spt5 C-terminal domain / Spt5 C-terminal nonapeptide repeat binding Spt4 / NGN domain / Transcription elongation factor SPT5 / Early transcription elongation factor of RNA pol II, NGN section / NusG, N-terminal / In Spt5p, this domain may confer affinity for Spt4p. It possesses a RNP-like fold. / NusG, N-terminal domain superfamily / Cathepsin B; Chain A / KOW (Kyprides, Ouzounis, Woese) motif. / Translation protein SH3-like domain superfamily / KOW motif / KOW / Ribosomal protein L2, domain 2 / Alpha-Beta Complex / Alpha Beta
Similarity search - Domain/homology
Transcription elongation factor SPT5 / RNA polymerase-associated protein RTF1 homolog
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.424 Å
AuthorsWier, A.D. / Heroux, A. / VanDemark, A.P.
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2013
Title: Structural basis for Spt5-mediated recruitment of the Paf1 complex to chromatin.
Authors: Wier, A.D. / Mayekar, M.K. / Heroux, A. / Arndt, K.M. / Vandemark, A.P.
History
DepositionJun 3, 2013Deposition site: RCSB / Processing site: RCSB
Revision 1.0Oct 2, 2013Provider: repository / Type: Initial release
Revision 1.1Oct 23, 2013Group: Database references
Revision 1.2Nov 20, 2013Group: Database references
Revision 1.3Nov 15, 2017Group: Refinement description / Category: software / Item: _software.classification / _software.name
Revision 1.4Sep 20, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_conn / struct_ref_seq_dif / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_leaving_atom_flag / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: RNA polymerase-associated protein RTF1 homolog
B: RNA polymerase-associated protein RTF1 homolog
C: RNA polymerase-associated protein RTF1 homolog
D: RNA polymerase-associated protein RTF1 homolog
E: RNA polymerase-associated protein RTF1 homolog
F: RNA polymerase-associated protein RTF1 homolog
G: Transcription elongation factor SPT5
H: Transcription elongation factor SPT5
I: Transcription elongation factor SPT5
J: Transcription elongation factor SPT5
hetero molecules


Theoretical massNumber of molelcules
Total (without water)104,56243
Polymers101,40810
Non-polymers3,15433
Water1,892105
1
B: RNA polymerase-associated protein RTF1 homolog
G: Transcription elongation factor SPT5
hetero molecules


Theoretical massNumber of molelcules
Total (without water)18,0649
Polymers17,3922
Non-polymers6727
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1820 Å2
ΔGint-83 kcal/mol
Surface area8470 Å2
MethodPISA
2
C: RNA polymerase-associated protein RTF1 homolog
H: Transcription elongation factor SPT5
hetero molecules


Theoretical massNumber of molelcules
Total (without water)17,8687
Polymers17,3922
Non-polymers4765
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1570 Å2
ΔGint-40 kcal/mol
Surface area8490 Å2
MethodPISA
3
E: RNA polymerase-associated protein RTF1 homolog
I: Transcription elongation factor SPT5
hetero molecules


Theoretical massNumber of molelcules
Total (without water)17,8727
Polymers17,3922
Non-polymers4805
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1740 Å2
ΔGint-74 kcal/mol
Surface area8170 Å2
MethodPISA
4
F: RNA polymerase-associated protein RTF1 homolog
J: Transcription elongation factor SPT5
hetero molecules


Theoretical massNumber of molelcules
Total (without water)17,7726
Polymers17,3922
Non-polymers3804
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1480 Å2
ΔGint-43 kcal/mol
Surface area8320 Å2
MethodPISA
5
A: RNA polymerase-associated protein RTF1 homolog
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,3015
Polymers15,9201
Non-polymers3804
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
6
D: RNA polymerase-associated protein RTF1 homolog
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,6859
Polymers15,9201
Non-polymers7658
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
Unit cell
Length a, b, c (Å)112.932, 172.514, 58.476
Angle α, β, γ (deg.)90.000, 107.000, 90.000
Int Tables number5
Space group name H-MC121

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Components

#1: Protein
RNA polymerase-associated protein RTF1 homolog


Mass: 15920.326 Da / Num. of mol.: 6 / Fragment: Plus3, UNP residues 353-484
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: KIAA0252, RTF1 / Plasmid: pLC3 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) Codon+RILP / References: UniProt: Q92541
#2: Protein/peptide
Transcription elongation factor SPT5 / hSPT5 / DRB sensitivity-inducing factor 160 kDa subunit / DSIF p160 / DRB sensitivity-inducing ...hSPT5 / DRB sensitivity-inducing factor 160 kDa subunit / DSIF p160 / DRB sensitivity-inducing factor large subunit / DSIF large subunit / Tat-cotransactivator 1 protein / Tat-CT1 protein


Mass: 1471.469 Da / Num. of mol.: 4 / Fragment: CTR, UNP residues 778-790 / Source method: obtained synthetically / Details: This sequence occurs naturally in human Spt5 / Source: (synth.) Homo sapiens (human) / References: UniProt: O00267
#3: Chemical...
ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 29 / Source method: obtained synthetically / Formula: SO4
#4: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C3H8O3
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 105 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.69 Å3/Da / Density % sol: 54.2 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 4.2
Details: Sodium acetate, Ammonium sulfate, pH 4.2, vapor diffusion, sitting drop, temperature 293K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: NSLS / Beamline: X25 / Wavelength: 1.1 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Aug 5, 2012
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.1 Å / Relative weight: 1
ReflectionResolution: 2.424→50 Å / Num. obs: 40281 / % possible obs: 99.9 % / Redundancy: 3.6 % / Rmerge(I) obs: 0.068 / Χ2: 0.665 / Net I/σ(I): 8.8
Reflection shell
Resolution (Å)Redundancy (%)Rmerge(I) obsNum. unique allΧ2Diffraction-ID% possible all
2.424-2.523.40.37640100.53199.9
2.52-2.623.60.32339920.498199.9
2.62-2.743.40.24840180.533199.9
2.74-2.883.70.18340400.5761100
2.88-3.063.50.12940270.6251100
3.06-3.33.60.08639970.808199.9
3.3-3.633.60.05540350.8831100
3.63-4.153.60.04440360.6891100
4.15-5.233.70.04240440.6981100
5.23-503.60.05940820.794199.9

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Phasing

PhasingMethod: molecular replacement
Phasing MR
Highest resolutionLowest resolution
Rotation2.42 Å46.92 Å
Translation2.42 Å46.92 Å

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Processing

Software
NameVersionClassificationNB
DENZOdata reduction
SCALEPACKdata scaling
PHASER2.5.0phasing
PHENIX1.8.2_1309refinement
PDB_EXTRACT3.11data extraction
HKL-2000data scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 4L1P

4l1p


Resolution: 2.424→46.923 Å / Occupancy max: 1 / Occupancy min: 1 / FOM work R set: 0.8414 / SU ML: 0.28 / σ(F): 1.38 / Phase error: 23.56 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2263 1750 4.94 %
Rwork0.1777 --
obs0.1801 40278 99.55 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 148.25 Å2 / Biso mean: 55.4372 Å2 / Biso min: 21.54 Å2
Refinement stepCycle: LAST / Resolution: 2.424→46.923 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6719 0 169 105 6993
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0096985
X-RAY DIFFRACTIONf_angle_d1.1169391
X-RAY DIFFRACTIONf_chiral_restr0.068997
X-RAY DIFFRACTIONf_plane_restr0.0041170
X-RAY DIFFRACTIONf_dihedral_angle_d17.8672611
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 14

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.424-2.48510.2951400.22652565270594
2.4851-2.55230.2721330.216927662899100
2.5523-2.62740.3081460.217427322878100
2.6274-2.71220.27591430.212927202863100
2.7122-2.80910.25741340.213827572891100
2.8091-2.92160.26121530.22127362889100
2.9216-3.05450.27221350.206827452880100
3.0545-3.21550.26611520.196627272879100
3.2155-3.41690.22381440.182527572901100
3.4169-3.68070.20721360.166827502886100
3.6807-4.05090.23261310.158527422873100
4.0509-4.63660.16761390.130327612900100
4.6366-5.83990.19651780.15827262904100
5.8399-46.9230.21871260.184728042930100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
15.04830.7881.1791.88320.71345.57990.0292-1.481.02021.4321-0.387-0.9551-1.18030.02730.04770.6321-0.1013-0.13090.5793-0.02150.7028275.7874-0.049179.2185
24.98240.71610.20954.32071.12280.25520.7264-1.7814-0.63431.2204-0.3623-0.37140.1082-0.174-0.11360.394-0.0635-0.02350.5140.09580.3524265.3339-9.401578.2365
32.5676-2.6430.52425.1452-2.6341.82640.0105-0.35870.21440.28650.33370.5694-0.4451-0.028-0.35540.3325-0.0233-0.03350.35720.05950.4697249.9511-10.86271.6449
48.82730.53580.72120.41330.20955.61460.0398-0.28350.4187-0.50080.44-0.4313-0.90780.5903-0.27360.2931-0.0013-0.00590.30080.09670.3591255.8236-3.146569.0058
56.5499-0.17690.64346.8011-1.37945.5321-0.47130.6968-0.7775-0.42190.4673-0.44430.04350.16610.02940.2548-0.10940.08790.4209-0.04920.3648263.9895-13.466165.4827
66.24472.61810.30758.0271-1.89856.2503-0.43880.59870.0883-1.05120.19930.15420.48760.1150.19460.3658-0.14710.00870.5372-0.00260.3972248.8302-18.038359.9895
72.19062.09580.48115.0443-1.47623.2005-0.27061.49890.2539-0.41110.71870.4456-0.45780.3042-0.38740.4105-0.15070.02540.5088-0.0210.3232256.3028-11.633462.1219
88.59173.95782.72373.21330.96720.9242-0.13322.0793-0.617-0.53090.0316-0.42510.62940.74520.08230.4401-0.28160.0431.1619-0.01250.2667266.7806-10.83559.146
99.54263.9218-0.53413.7274-0.99370.6556-0.15550.2467-1.062-0.18680.0102-0.67120.0950.22630.10810.2772-0.0251-0.00780.3290.00240.4037269.7808-13.940770.2075
103.5978-3.08440.26745.75843.01373.7481-0.35620.22990.3758-0.08880.25920.3426-0.85060.3660.19850.2862-0.0834-0.00390.4105-0.02250.3522274.4072-0.548368.8842
112.18341.33880.71073.95750.67025.39880.8597-0.37741.39870.2825-0.11730.6592-0.0375-0.7234-0.78090.5506-0.05590.12760.6190.00550.4564259.86420.348878.6355
123.1505-0.9865-0.33615.7369-1.98375.15080.40030.73480.5894-0.5445-0.07340.7132-0.6883-0.3094-0.26160.4790.08920.05760.42040.08560.4782231.102210.589661.4946
135.0011-0.38270.77515.8116-1.67092.92550.01990.2999-0.5601-1.03010.1580.16820.3331-0.0876-0.18240.3612-0.0470.00320.2908-0.06190.316229.9571-7.945368.0584
145.1242-3.22030.63043.0954-1.71015.325-0.1484-0.5567-0.10090.0002-0.0008-1.5272-0.22760.60450.00520.2488-0.02970.01030.52050.03420.3775238.3975-5.77574.2331
152.0144-0.8466-0.99511.362-0.42943.834-0.0539-0.3203-0.02180.3712-0.29570.8647-0.4299-0.64940.12330.36810.06990.13120.4376-0.130.7079216.36111.377872.8452
162.35480.80732.92875.7529-0.02256.08920.3068-0.91640.78740.2711-0.17380.0458-0.4028-0.1751-0.18860.2895-0.02960.10030.3512-0.00930.2986224.41171.319175.2976
173.56852.54213.03064.58644.73365.02550.6607-0.546-0.5492-0.22740.4203-0.653-0.49820.577-1.09640.4491-0.1424-0.00290.4060.00390.4152225.3876-13.451781.7941
183.1760.33151.33315.5113-0.81674.87530.211-0.13760.05550.3066-0.15630.0866-0.42580.00630.00450.29870.01960.03960.3077-0.06890.2653225.3811-1.707576.3249
190.9088-0.1205-0.76488.38661.41721.05040.02770.02260.57710.3772-0.23380.6276-0.2108-0.05410.23970.5262-0.0050.11090.29390.02830.3669225.104114.510469.7268
204.9112-5.0990.11875.3614-1.22988.55010.80890.12860.3419-1.4701-0.3433-0.7177-0.02390.3585-0.35840.39920.04240.06130.3266-0.01070.4243240.44563.118863.237
212.14560.08220.38614.43741.21382.4260.1427-0.01090.0635-0.0316-0.1838-0.00020.1865-0.19370.04620.2465-0.0432-0.00810.28370.00680.2611244.7786-35.023571.2057
223.9246-1.123-1.71157.55392.77264.60040.1584-0.3290.04510.30640.0733-0.37940.3250.0948-0.23770.26-0.041-0.07180.34940.03130.3275248.4786-35.172376.0402
232.24310.26880.93894.6054-1.66782.9320.2088-0.3245-0.42790.2676-0.0939-1.0365-0.21420.0612-0.02460.5847-0.0247-0.12120.2934-0.030.5981249.5419-50.93968.7492
247.3276-3.9533-1.23673.05173.14056.76710.7516-0.2040.1119-0.7579-0.23910.4463-0.2458-0.2734-0.47540.4654-0.0137-0.05160.30240.040.4714233.8177-39.590662.6019
255.32551.4661-2.67322.44830.27971.87710.1848-0.76270.03080.1281-0.15320.13690.17590.0338-0.03190.3497-0.0427-0.03870.4279-0.0530.2259210.3868-27.588876.7355
268.34-1.956-0.46771.36270.98612.70460.0784-0.2092-0.4771-0.26320.0005-0.19440.51970.2639-0.04520.36030.0142-0.0850.3123-0.08710.4333222.1394-32.398168.8805
276.39141.316-2.22697.03792.68794.5810.35730.15041.5251-1.1339-0.010.9238-0.6658-0.6848-0.21620.4189-0.0264-0.11050.52210.02410.5952206.4529-15.562865.6657
288.3099-3.0926-0.93622.82893.04016.0076-0.24030.26520.3641-0.27250.652-0.03180.0813-0.485-0.36080.3355-0.06640.0030.39970.06030.2682210.0799-22.986964.6199
297.5257-0.0465-1.16667.897-0.80898.0328-0.5041.45760.9544-1.14560.0692-1.1218-0.0729-0.53960.11940.4148-0.09590.07770.65-0.05920.5534225.2479-18.534659.5624
302.89090.251-0.37184.81830.42633.7664-0.16831.83980.5613-0.55990.7299-0.43840.2654-0.0908-0.35970.4545-0.13580.04540.5514-0.07970.3276217.7159-25.007761.856
317.37014.4086-2.24973.1064-1.43030.7268-0.61120.08390.1403-0.74510.50080.15850.6611-1.31050.02740.5276-0.35230.03630.84850.13090.2022207.2352-25.966159.0476
322.71731.4642-0.08847.15581.66572.89540.19410.29511.29510.05790.22150.0670.1043-0.0769-0.29740.25440.0131-0.07860.3345-0.05490.3225213.0217-18.325869.657
337.93615.16981.8999.88473.75153.60550.15160.23471.06270.5079-0.230.88110.0773-0.00070.13580.3019-0.09150.0370.34820.02750.3487197.0899-25.380770.3088
342.3787-3.05873.00434.796-3.91183.63890.04130.1163-0.999-0.138-0.13250.25360.54120.09540.040.347-0.121-0.02340.6774-0.04860.3189199.5196-35.56869.2005
352.15423.35280.51826.17150.99242.01710.6333-1.0328-0.52370.8759-0.6421-0.5080.6010.9189-0.00180.4986-0.0013-0.0270.6620.10590.3402214.818-36.536678.9889
365.3940.23062.4592.8012-1.13152.00370.1443-1.421-1.0778-0.00390.42860.3496-0.0987-0.3389-0.49430.4759-0.03640.10870.47870.07180.329239.60571.224452.1561
373.7484-2.841-0.06166.41310.30034.0179-0.556-0.31770.72720.2840.2752-0.0391-0.7250.04330.21440.5334-0.0105-0.03910.3377-0.02440.4682233.535619.378243.6496
386.0661-0.9347-1.34194.9484-1.54524.46280.05490.4601-0.9074-0.240.09080.1875-0.0727-0.0877-0.15250.36020.02520.05270.3279-0.10730.3339231.97036.280934.721
394.1242-2.9242-1.06087.2721.0553.93950.25020.2591-0.0192-0.7939-0.38830.5337-0.60580.09530.16650.4507-0.0029-0.04530.3402-0.02310.3115232.200414.010434.2377
402.1898-1.5224-0.97873.85841.03133.68540.1340.5748-0.80950.44430.01910.4660.3075-0.2134-0.2450.3331-0.004-0.01270.3175-0.00920.4742238.9506-3.672940.7689
413.7757-1.4835-0.64275.25033.03776.899-0.2459-0.2070.14730.37630.5148-0.2996-0.16020.8094-0.23790.3762-0.04590.06660.3853-0.02250.3568242.769.072450.7659
422.8981-1.2157-1.28698.18262.25143.0003-0.3453-0.1935-0.20510.34360.3569-0.27870.4340.1473-0.07030.39850.0275-0.03880.37460.06170.3047238.3442-49.006645.7999
436.49890.2989-0.26744.0612.09282.9052-0.06230.60270.6461-0.53810.2208-0.6137-0.1099-0.0951-0.20460.44710.10560.01360.47090.11120.366241.6215-42.094833.8031
446.7985-3.2150.46076.36422.72741.9401-0.05610.9511-0.3995-0.9951-1.2436-0.29150.09860.11680.59890.62960.06780.1080.43530.22940.7661245.859-58.791529.0913
453.2472-2.3970.23826.45690.93894.28830.05290.59650.1206-0.5001-0.2656-0.47010.52650.02180.25060.48140.01860.11460.45630.05160.3797240.2382-48.161934.1273
462.1167-2.3710.06445.6628-1.61682.86370.37380.84571.00280.27360.0683-0.4359-0.37870.0171-0.10160.37680.10520.08580.39180.05310.4848235.0839-32.478840.1717
474.2712-3.85582.19383.3953-3.1495.6489-0.2133-0.39950.0432-0.01070.17930.12570.2811-0.41430.08390.4509-0.0258-0.0880.44890.04040.3885231.5286-45.379150.0008
482.08150.0186-0.29247.0272-1.8186.32090.3162-0.17260.20930.3771-0.32820.4612-0.001-0.8235-0.12690.4146-0.00940.04580.5064-0.04020.5552216.2995-6.827372.5796
492.21970.42530.477.37733.2663.6525-0.0373-1.02050.36670.3388-0.1033-1.1861-1.30451.01070.24570.42-0.1336-0.08130.6212-0.08080.5933258.3094-30.130673.6054
501.67292.18951.44538.2284-0.29322.17170.88730.53040.7871-0.00120.7568-0.1038-1.1834-1.509-1.45470.7210.04010.01180.82650.14330.8762223.80718.995133.0444
515.395-0.4402-2.62552.19841.78488.70981.1486-0.92070.7768-1.8953-1.0075-0.78570.71971.64540.26190.9650.05080.23390.88510.01570.8449252.3241-44.962833.3636
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 349 through 355 )A0
2X-RAY DIFFRACTION2chain 'A' and (resid 356 through 365 )A0
3X-RAY DIFFRACTION3chain 'A' and (resid 366 through 377 )A0
4X-RAY DIFFRACTION4chain 'A' and (resid 378 through 385 )A0
5X-RAY DIFFRACTION5chain 'A' and (resid 386 through 410 )A0
6X-RAY DIFFRACTION6chain 'A' and (resid 411 through 419 )A0
7X-RAY DIFFRACTION7chain 'A' and (resid 420 through 430 )A0
8X-RAY DIFFRACTION8chain 'A' and (resid 431 through 437 )A0
9X-RAY DIFFRACTION9chain 'A' and (resid 438 through 461 )A0
10X-RAY DIFFRACTION10chain 'A' and (resid 462 through 468 )A0
11X-RAY DIFFRACTION11chain 'A' and (resid 469 through 483 )A0
12X-RAY DIFFRACTION12chain 'B' and (resid 350 through 362 )B0
13X-RAY DIFFRACTION13chain 'B' and (resid 363 through 372 )B0
14X-RAY DIFFRACTION14chain 'B' and (resid 373 through 385 )B0
15X-RAY DIFFRACTION15chain 'B' and (resid 386 through 396 )B0
16X-RAY DIFFRACTION16chain 'B' and (resid 397 through 410 )B0
17X-RAY DIFFRACTION17chain 'B' and (resid 411 through 419 )B0
18X-RAY DIFFRACTION18chain 'B' and (resid 420 through 448 )B0
19X-RAY DIFFRACTION19chain 'B' and (resid 449 through 468 )B0
20X-RAY DIFFRACTION20chain 'B' and (resid 469 through 483 )B0
21X-RAY DIFFRACTION21chain 'C' and (resid 350 through 419 )C0
22X-RAY DIFFRACTION22chain 'C' and (resid 420 through 448 )C0
23X-RAY DIFFRACTION23chain 'C' and (resid 449 through 468 )C0
24X-RAY DIFFRACTION24chain 'C' and (resid 469 through 483 )C0
25X-RAY DIFFRACTION25chain 'D' and (resid 349 through 372 )D0
26X-RAY DIFFRACTION26chain 'D' and (resid 373 through 385 )D0
27X-RAY DIFFRACTION27chain 'D' and (resid 386 through 396 )D0
28X-RAY DIFFRACTION28chain 'D' and (resid 397 through 410 )D0
29X-RAY DIFFRACTION29chain 'D' and (resid 411 through 419 )D0
30X-RAY DIFFRACTION30chain 'D' and (resid 420 through 430 )D0
31X-RAY DIFFRACTION31chain 'D' and (resid 431 through 437 )D0
32X-RAY DIFFRACTION32chain 'D' and (resid 438 through 448 )D0
33X-RAY DIFFRACTION33chain 'D' and (resid 449 through 461 )D0
34X-RAY DIFFRACTION34chain 'D' and (resid 462 through 468 )D0
35X-RAY DIFFRACTION35chain 'D' and (resid 469 through 484 )D0
36X-RAY DIFFRACTION36chain 'E' and (resid 349 through 362 )E0
37X-RAY DIFFRACTION37chain 'E' and (resid 363 through 385 )E0
38X-RAY DIFFRACTION38chain 'E' and (resid 386 through 410 )E0
39X-RAY DIFFRACTION39chain 'E' and (resid 411 through 448 )E0
40X-RAY DIFFRACTION40chain 'E' and (resid 449 through 461 )E0
41X-RAY DIFFRACTION41chain 'E' and (resid 462 through 482 )E0
42X-RAY DIFFRACTION42chain 'F' and (resid 349 through 385 )F0
43X-RAY DIFFRACTION43chain 'F' and (resid 386 through 410 )F0
44X-RAY DIFFRACTION44chain 'F' and (resid 411 through 419 )F0
45X-RAY DIFFRACTION45chain 'F' and (resid 420 through 448 )F0
46X-RAY DIFFRACTION46chain 'F' and (resid 449 through 461 )F0
47X-RAY DIFFRACTION47chain 'F' and (resid 462 through 482 )F0
48X-RAY DIFFRACTION48chain 'G' and (resid 781 through 788 )G0
49X-RAY DIFFRACTION49chain 'H' and (resid 780 through 790 )H0
50X-RAY DIFFRACTION50chain 'I' and (resid 783 through 787 )I0
51X-RAY DIFFRACTION51chain 'J' and (resid 782 through 786 )J0

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