- PDB-4kp4: Deciphering cis-trans directionality and visualizing autophosphor... -
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ID or keywords:
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Basic information
Entry
Database: PDB / ID: 4kp4
Title
Deciphering cis-trans directionality and visualizing autophosphorylation in histidine kinases.
Components
Osmolarity sensor protein EnvZ, Histidine kinase
Keywords
TRANSFERASE/SIGNALING PROTEIN / Four helix-bundle / Bergerat fold / Kinase and phosphotransferase / ATP binding / TRANSFERASE-SIGNALING PROTEIN complex
Function / homology
Function and homology information
histidine phosphotransfer kinase activity / response to osmotic stress / histidine kinase / phosphorelay signal transduction system / phosphoprotein phosphatase activity / phosphorelay sensor kinase activity / outer membrane-bounded periplasmic space / protein autophosphorylation / phosphorylation / nucleotide binding ...histidine phosphotransfer kinase activity / response to osmotic stress / histidine kinase / phosphorelay signal transduction system / phosphoprotein phosphatase activity / phosphorelay sensor kinase activity / outer membrane-bounded periplasmic space / protein autophosphorylation / phosphorylation / nucleotide binding / signal transduction / protein homodimerization activity / ATP binding / identical protein binding / metal ion binding / plasma membrane / cytosol Similarity search - Function
Resolution: 3→3.16 Å / Redundancy: 10.7 % / Rmerge(I) obs: 0.939 / Mean I/σ(I) obs: 2.3 / % possible all: 98.5
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Processing
Software
Name
Version
Classification
PHASER
phasing
REFMAC
5.7.0032
refinement
XDS
datareduction
SCALA
datascaling
Refinement
Method to determine structure: SAD / Resolution: 3→29.58 Å / Cor.coef. Fo:Fc: 0.961 / Cor.coef. Fo:Fc free: 0.942 / SU B: 27.484 / SU ML: 0.237 / Cross valid method: THROUGHOUT / ESU R: 0.462 / ESU R Free: 0.303 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.234
1034
5 %
RANDOM
Rwork
0.194
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obs
0.196
19482
97.1 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK