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Yorodumi- PDB-4jbw: Crystal structure of E. coli maltose transporter MalFGK2 in compl... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4jbw | ||||||
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Title | Crystal structure of E. coli maltose transporter MalFGK2 in complex with its regulatory protein EIIAglc | ||||||
Components |
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Keywords | TRANSPORT PROTEIN / ABC transporter ATPase inducer exclusion carbon catabolite repression | ||||||
Function / homology | Function and homology information negative regulation of carbohydrate metabolic process / ABC-type maltose transporter / ABC-type maltose transporter activity / negative regulation of maltose transport / enzyme IIA-maltose transporter complex / negative regulation of transmembrane transport / phosphoenolpyruvate-dependent sugar phosphotransferase system / transmembrane transporter complex / maltose transport complex / maltose transport ...negative regulation of carbohydrate metabolic process / ABC-type maltose transporter / ABC-type maltose transporter activity / negative regulation of maltose transport / enzyme IIA-maltose transporter complex / negative regulation of transmembrane transport / phosphoenolpyruvate-dependent sugar phosphotransferase system / transmembrane transporter complex / maltose transport complex / maltose transport / maltodextrin transmembrane transport / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / ATP-binding cassette (ABC) transporter complex / kinase activity / DNA-binding transcription factor binding / phosphorylation / DNA damage response / ATP hydrolysis activity / ATP binding / membrane / metal ion binding / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.913 Å | ||||||
Authors | Chen, S. / Oldham, M.L. / Davidson, A.L. / Chen, J. | ||||||
Citation | Journal: Nature / Year: 2013 Title: Carbon catabolite repression of the maltose transporter revealed by X-ray crystallography. Authors: Chen, S. / Oldham, M.L. / Davidson, A.L. / Chen, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4jbw.cif.gz | 698.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4jbw.ent.gz | 569.8 KB | Display | PDB format |
PDBx/mmJSON format | 4jbw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jb/4jbw ftp://data.pdbj.org/pub/pdb/validation_reports/jb/4jbw | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Details | Biological unit: chains H,I,C,D,O, and P (2nd equivalent NCS mate) |
-Components
#1: Protein | Mass: 57052.898 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12 / Gene: malF, b4033, JW3993 / Production host: Escherichia coli (E. coli) / References: UniProt: P02916 #2: Protein | Mass: 32246.227 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12 / Gene: malG, b4032, JW3992 / Production host: Escherichia coli (E. coli) / References: UniProt: P68183 #3: Protein | Mass: 42184.535 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12 / Gene: malK, b4035, JW3995 / Production host: Escherichia coli (E. coli) / References: UniProt: P68187, EC: 3.6.3.19 #4: Protein | Mass: 18545.289 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12 / Gene: crr, gsr, iex, tgs, treD, b2417, JW2410 / Production host: Escherichia coli (E. coli) References: UniProt: P69783, Transferases; Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor #5: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.06 Å3/Da / Density % sol: 69.71 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop / pH: 5.6 Details: 13% PEG monomethylether 2000, 100mM Sodium cacodylate pH 5.6, 100mM Non Detergent Sulfobetaine (NDSB-256), VAPOR DIFFUSION, SITTING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 23-ID-B / Wavelength: 1.0332 Å |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Oct 12, 2012 |
Radiation | Monochromator: double crystal monochromator and vertically focusing mirror Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0332 Å / Relative weight: 1 |
Reflection | Resolution: 3.913→20 Å / Num. all: 59277 / Num. obs: 59277 / % possible obs: 95 % / Redundancy: 4.9 % / Rsym value: 0.106 / Net I/σ(I): 13.8 |
Reflection shell | Resolution: 3.913→4.06 Å / % possible all: 96.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1F3G, 3FH6, and 3PV0 Resolution: 3.913→19.975 Å / SU ML: 0.6 / σ(F): 1.33 / Phase error: 31.05 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.913→19.975 Å
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Refine LS restraints |
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LS refinement shell |
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