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- PDB-4hfe: The GLIC pentameric Ligand-Gated Ion Channel F14'A ethanol-sensit... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4hfe | ||||||
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Title | The GLIC pentameric Ligand-Gated Ion Channel F14'A ethanol-sensitive mutant complexed to ethanol | ||||||
![]() | Proton-gated ion channel | ||||||
![]() | MEMBRANE PROTEIN / TRANSPORT PROTEIN / pentameric transmembrane channel / Ion-channel | ||||||
Function / homology | ![]() sodium channel activity / extracellular ligand-gated monoatomic ion channel activity / potassium channel activity / transmembrane transporter complex / regulation of membrane potential / transmembrane signaling receptor activity / neuron projection / signal transduction / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Sauguet, L. / Howard, R.J. / Malherbe, L. / Lee, U.S. / Corringer, P.J. / Harris, R.A. / Delarue, M. | ||||||
![]() | ![]() Title: Structural basis for potentiation by alcohols and anaesthetics in a ligand-gated ion channel. Authors: Sauguet, L. / Howard, R.J. / Malherbe, L. / Lee, U.S. / Corringer, P.J. / Harris, R.A. / Delarue, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 657.6 KB | Display | ![]() |
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PDB format | ![]() | 551.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.9 MB | Display | ![]() |
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Full document | ![]() | 1.9 MB | Display | |
Data in XML | ![]() | 59.4 KB | Display | |
Data in CIF | ![]() | 78.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Details | homo-pentamer |
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Components
-Protein / Sugars , 2 types, 11 molecules ABCDE![](data/chem/img/LMT.gif)
![](data/chem/img/LMT.gif)
#1: Protein | Mass: 36215.652 Da / Num. of mol.: 5 / Fragment: unp residues 44-359 / Mutation: F280A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #6: Sugar | ChemComp-LMT / |
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-Non-polymers , 7 types, 149 molecules ![](data/chem/img/ACT.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/EOH.gif)
![](data/chem/img/PLC.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/EOH.gif)
![](data/chem/img/PLC.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-ACT / #3: Chemical | ChemComp-CL / #4: Chemical | ChemComp-NA / #5: Chemical | ChemComp-EOH / #7: Chemical | ChemComp-PLC / #8: Chemical | ChemComp-UNL / Num. of mol.: 10 / Source method: obtained synthetically #9: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 5.2 Å3/Da / Density % sol: 73.2 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 4 Details: 12-15% PEG4K, 0.1M Na Acetate, 0.4M NaSCN, 0.2M Ethanol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 15, 2011 Details: Kirkpatrick-Baez pair of bi-morph mirrors plus channel cut cryogenically cooled monochromator crystal |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9194 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→49.5 Å / Num. obs: 90680 / % possible obs: 99.6 % / Redundancy: 3.1 % / Biso Wilson estimate: 74.01 Å2 / Rmerge(I) obs: 0.069 / Rsym value: 0.047 / Net I/σ(I): 11 |
Reflection shell | Resolution: 2.8→2.95 Å / Redundancy: 3.1 % / Rmerge(I) obs: 0.6 / Mean I/σ(I) obs: 2.2 / Rsym value: 0.407 / % possible all: 99.4 |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 86.33 Å2
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Refine analyze | Luzzati coordinate error obs: 0.419 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→30 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.8→2.87 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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