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- PDB-3uu8: The GLIC pentameric Ligand-Gated Ion Channel Loop2-24' mutant red... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3uu8 | ||||||
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Title | The GLIC pentameric Ligand-Gated Ion Channel Loop2-24' mutant reduced in solution | ||||||
![]() | Glr4197 protein | ||||||
![]() | MEMBRANE PROTEIN / TRANSPORT PROTEIN / Cys-loop receptor family | ||||||
Function / homology | ![]() sodium channel activity / extracellular ligand-gated monoatomic ion channel activity / potassium channel activity / transmembrane transporter complex / regulation of membrane potential / transmembrane signaling receptor activity / neuron projection / signal transduction / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Sauguet, L. / Nury, H. / Corringer, P.J. / Delarue, M. | ||||||
![]() | ![]() Title: A locally closed conformation of a bacterial pentameric proton-gated ion channel. Authors: Prevost, M.S. / Sauguet, L. / Nury, H. / Van Renterghem, C. / Huon, C. / Poitevin, F. / Baaden, M. / Delarue, M. / Corringer, P.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 329.2 KB | Display | ![]() |
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PDB format | ![]() | 267.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 3.5 MB | Display | ![]() |
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Full document | ![]() | 3.6 MB | Display | |
Data in XML | ![]() | 60.5 KB | Display | |
Data in CIF | ![]() | 79.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3tlsC ![]() 3tltC ![]() 3tluC ![]() 3tlvC ![]() 3tlwC ![]() 3uu3C ![]() 3uu4C ![]() 3uu5C ![]() 3uu6C ![]() 3uubC ![]() 3eamS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein / Sugars , 2 types, 11 molecules ABCDE![](data/chem/img/LMT.gif)
![](data/chem/img/LMT.gif)
#1: Protein | Mass: 36509.898 Da / Num. of mol.: 5 / Fragment: UNP residues 44-359 / Mutation: C27S,K33C,K248C Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #4: Sugar | ChemComp-LMT / |
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-Non-polymers , 4 types, 72 molecules ![](data/chem/img/PLC.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-PLC / #3: Chemical | ChemComp-CL / #5: Chemical | ChemComp-NA / | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 5.18 Å3/Da / Density % sol: 76.26 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 4 Details: 15% PEG4000, 0.4 M sodium thiocyanate, 0.1 M sodium acetate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Apr 18, 2009 | ||||||||||||||||||
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||
Radiation wavelength | Wavelength: 0.98011 Å / Relative weight: 1 | ||||||||||||||||||
Reflection | Resolution: 3.25→34.11 Å / Num. all: 58722 / Num. obs: 57137 / % possible obs: 97.3 % / Redundancy: 2.9 % / Biso Wilson estimate: 81.73 Å2 / Rmerge(I) obs: 0.116 / Rsym value: 0.078 / Net I/σ(I): 8.2 | ||||||||||||||||||
Reflection shell | Diffraction-ID: 1 / Redundancy: 2.9 %
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 3EAM Resolution: 3.25→34.11 Å / Cor.coef. Fo:Fc: 0.8462 / Cor.coef. Fo:Fc free: 0.8358 / SU R Cruickshank DPI: 1.202 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.908 / SU Rfree Blow DPI: 0.349 / SU Rfree Cruickshank DPI: 0.366
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Displacement parameters | Biso mean: 63.43 Å2
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Refine analyze | Luzzati coordinate error obs: 0.631 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.25→34.11 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.25→3.33 Å / Total num. of bins used: 20
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