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- PDB-3uu4: The GLIC pentameric Ligand-Gated Ion Channel Loop2-21' mutant red... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3uu4 | ||||||
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Title | The GLIC pentameric Ligand-Gated Ion Channel Loop2-21' mutant reduced in the crystal in a locally-closed conformation (LC1 subtype) | ||||||
![]() | Glr4197 protein | ||||||
![]() | MEMBRANE PROTEIN / TRANSPORT PROTEIN / Cys-loop receptor family | ||||||
Function / homology | ![]() sodium channel activity / extracellular ligand-gated monoatomic ion channel activity / potassium channel activity / transmembrane transporter complex / regulation of membrane potential / transmembrane signaling receptor activity / neuron projection / signal transduction / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Sauguet, L. / Nury, H. / Corringer, P.J. / Delarue, M. | ||||||
![]() | ![]() Title: A locally closed conformation of a bacterial pentameric proton-gated ion channel. Authors: Prevost, M.S. / Sauguet, L. / Nury, H. / Van Renterghem, C. / Huon, C. / Poitevin, F. / Baaden, M. / Delarue, M. / Corringer, P.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 313.8 KB | Display | ![]() |
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PDB format | ![]() | 257.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 610.4 KB | Display | ![]() |
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Full document | ![]() | 633.7 KB | Display | |
Data in XML | ![]() | 53.3 KB | Display | |
Data in CIF | ![]() | 73.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3tlsC ![]() 3tltC ![]() 3tluC ![]() 3tlvC ![]() 3tlwC ![]() 3uu3C ![]() 3uu5C ![]() 3uu6C ![]() 3uu8C ![]() 3uubC ![]() 3eamS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 36524.977 Da / Num. of mol.: 5 / Fragment: UNP residues 44-359 / Mutation: C27S,K33C,N245C Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Sugar | ChemComp-LMT / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 5.2 Å3/Da / Density % sol: 76.34 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 4 Details: 15% PEG4000, 0.4 M sodium thiocyanate, 0.1 M sodium acetate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Feb 2, 2011 | ||||||||||||||||||
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||
Radiation wavelength | Wavelength: 0.98011 Å / Relative weight: 1 | ||||||||||||||||||
Reflection | Resolution: 3.05→40.9 Å / Num. all: 71200 / Num. obs: 70985 / % possible obs: 99.7 % / Redundancy: 3.7 % / Biso Wilson estimate: 90.4 Å2 / Rmerge(I) obs: 0.07 / Rsym value: 0.043 / Net I/σ(I): 10.8 | ||||||||||||||||||
Reflection shell | Diffraction-ID: 1 / Redundancy: 3.7 %
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 3EAM Resolution: 3.05→27.28 Å / Cor.coef. Fo:Fc: 0.8574 / Cor.coef. Fo:Fc free: 0.8392 / SU R Cruickshank DPI: 0.531 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.473 / SU Rfree Blow DPI: 0.296 / SU Rfree Cruickshank DPI: 0.311
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Displacement parameters | Biso mean: 104.69 Å2
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Refine analyze | Luzzati coordinate error obs: 0.71 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.05→27.28 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.05→3.13 Å / Total num. of bins used: 20
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