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- PDB-2xqa: Pentameric ligand gated ion channel GLIC in complex with tetrabut... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2xqa | ||||||
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Title | Pentameric ligand gated ion channel GLIC in complex with tetrabutylantimony (TBSb) | ||||||
![]() | GLR4197 PROTEIN | ||||||
![]() | MEMBRANE PROTEIN / OPEN CHANNEL BLOCK | ||||||
Function / homology | ![]() sodium channel activity / extracellular ligand-gated monoatomic ion channel activity / potassium channel activity / transmembrane transporter complex / regulation of membrane potential / transmembrane signaling receptor activity / neuron projection / signal transduction / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Hilf, R.J.C. / Bertozzi, C. / Zimmermann, I. / Reiter, A. / Trauner, D. / Dutzler, R. | ||||||
![]() | ![]() Title: Structural Basis of Open Channel Block in a Prokaryotic Pentameric Ligand-Gated Ion Channel Authors: Hilf, R.J.C. / Bertozzi, C. / Zimmermann, I. / Reiter, A. / Trauner, D. / Dutzler, R. #1: ![]() Title: Structure of a Potentially Open State of a Proton-Activated Pentameric Ligand-Gated Ion Channel. Authors: Hilf, R.J.C. / Dutzler, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 624.3 KB | Display | ![]() |
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PDB format | ![]() | 523.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 472 KB | Display | ![]() |
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Full document | ![]() | 594.3 KB | Display | |
Data in XML | ![]() | 70.2 KB | Display | |
Data in CIF | ![]() | 92.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2xq3C ![]() 2xq4C ![]() 2xq5C ![]() 2xq6C ![]() 2xq7C ![]() 2xq8C ![]() 2xq9C C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
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Components
#1: Protein | Mass: 36277.723 Da / Num. of mol.: 5 / Fragment: RESIDUES 43-359 Source method: isolated from a genetically manipulated source Details: SB ION Source: (gene. exp.) ![]() Plasmid: PET26 / Production host: ![]() ![]() #2: Chemical | ChemComp-SB / | Sequence details | RESIDUES 50-359 | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 5.31 Å3/Da / Density % sol: 76.67 % / Description: NONE |
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Crystal grow | Temperature: 277.15 K / Method: vapor diffusion, hanging drop / pH: 4 Details: 9% PEG4000, 50MM CH3COONA, 200MM (NH4)2SO4, PH4.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277.15K, VAPOR DIFFUSION, HANGING DROP |
-Data collection
Diffraction | Mean temperature: 277.15 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 15, 2008 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.8 Å / Relative weight: 1 |
Reflection | Resolution: 3.6→40.2 Å / Num. obs: 43105 / % possible obs: 99.4 % / Observed criterion σ(I): 2.2 / Redundancy: 5.6 % / Biso Wilson estimate: 92.5 Å2 / Rmerge(I) obs: 0.09 |
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Processing
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Refinement | Method to determine structure: OTHER Starting model: NONE Resolution: 3.7→40.2 Å / SU ML: 0.59 / σ(F): 1.9 / Phase error: 31.41 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 50.467 Å2 / ksol: 0.276 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 3.7→40.2 Å
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Refine LS restraints |
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Refine LS restraints NCS |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 24.9164 Å / Origin y: 3.1883 Å / Origin z: 52.6059 Å
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Refinement TLS group | Selection details: ALL |