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- PDB-2xq6: Pentameric ligand gated ion channel GLIC in complex with cesium i... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2xq6 | ||||||
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Title | Pentameric ligand gated ion channel GLIC in complex with cesium ion (Cs+) | ||||||
![]() | GLR4197 PROTEIN | ||||||
![]() | MEMBRANE PROTEIN / OPEN CHANNEL BLOCK | ||||||
Function / homology | ![]() sodium channel activity / potassium channel activity / transmembrane transporter complex / extracellular ligand-gated monoatomic ion channel activity / transmembrane signaling receptor activity / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Hilf, R.J.C. / Bertozzi, C. / Zimmermann, I. / Reiter, A. / Trauner, D. / Dutzler, R. | ||||||
![]() | ![]() Title: Structural Basis of Open Channel Block in a Prokaryotic Pentameric Ligand-Gated Ion Channel Authors: Hilf, R.J.C. / Bertozzi, C. / Zimmermann, I. / Reiter, A. / Trauner, D. / Dutzler, R. #1: ![]() Title: Structure of a Potentially Open State of a Proton-Activated Pentameric Ligand-Gated Ion Channel. Authors: Hilf, R.J.C. / Dutzler, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 636.4 KB | Display | ![]() |
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PDB format | ![]() | 536.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2xq3C ![]() 2xq4C ![]() 2xq5C ![]() 2xq7C ![]() 2xq8C ![]() 2xq9C ![]() 2xqaC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
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Components
#1: Protein | Mass: 36277.723 Da / Num. of mol.: 5 / Fragment: RESIDUES 43-359 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Plasmid: PET26 / Production host: ![]() ![]() #2: Chemical | ChemComp-CS / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 5.16 Å3/Da / Density % sol: 76.2 % / Description: NONE |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 4 Details: 9% PEG4000, 50MM CH3COONA, 225MM (NH4)2SO4, PH4.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277.15K, VAPOR DIFFUSION, HANGING DROP |
-Data collection
Diffraction | Mean temperature: 90 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 10, 2009 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.8 Å / Relative weight: 1 |
Reflection | Resolution: 3.7→50 Å / Num. obs: 40144 / % possible obs: 88.9 % / Observed criterion σ(I): 2.1 / Redundancy: 3 % / Rmerge(I) obs: 0.14 / Net I/σ(I): 8.9 |
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Processing
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Refinement | Method to determine structure: OTHER Starting model: NONE Resolution: 3.7→39.866 Å / SU ML: 0.42 / σ(F): 1.91 / Phase error: 29.54 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 65.903 Å2 / ksol: 0.28 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 3.7→39.866 Å
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Refine LS restraints |
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Refine LS restraints NCS |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 24.8657 Å / Origin y: 3.6724 Å / Origin z: 52.6135 Å
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Refinement TLS group | Selection details: ALL |