|Entry||Database: PDB / ID: 4h5n|
|Title||HSC70 NBD with PO4, Na, Cl|
|Components||Heat shock cognate 71 kDa protein|
|Keywords||TRANSCRIPTION / Nucleotide Binding Domain|
|Function / homology|
Function and homology information
regulation of protein import / lumenal side of lysosomal membrane / negative regulation of supramolecular fiber organization / protein carrier chaperone / positive regulation by host of viral genome replication / clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane / postsynaptic specialization membrane / Lipophagy / GABA synthesis, release, reuptake and degradation / slow axonal transport ...regulation of protein import / lumenal side of lysosomal membrane / negative regulation of supramolecular fiber organization / protein carrier chaperone / positive regulation by host of viral genome replication / clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane / postsynaptic specialization membrane / Lipophagy / GABA synthesis, release, reuptake and degradation / slow axonal transport / chaperone-mediated autophagy translocation complex disassembly / clathrin-uncoating ATPase activity / protein targeting to lysosome involved in chaperone-mediated autophagy / glycinergic synapse / late endosomal microautophagy / regulation of postsynapse organization / CHL1 interactions / regulation of protein complex stability / C3HC4-type RING finger domain binding / ATP-dependent protein disaggregase activity / photoreceptor ribbon synapse / membrane organization / presynaptic cytosol / chaperone complex / Prp19 complex / positive regulation of mRNA splicing, via spliceosome / Lysosome Vesicle Biogenesis / misfolded protein binding / postsynaptic cytosol / Golgi Associated Vesicle Biogenesis / chaperone-mediated autophagy / cellular response to steroid hormone stimulus / phosphatidylserine binding / non-chaperonin molecular chaperone ATPase / chaperone cofactor-dependent protein refolding / autophagosome / Protein methylation / HSF1-dependent transactivation / response to unfolded protein / ATP metabolic process / Regulation of HSF1-mediated heat shock response / regulation of protein-containing complex assembly / Attenuation phase / cellular response to unfolded protein / heat shock protein binding / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / protein folding chaperone / mRNA Splicing - Major Pathway / lysosomal lumen / cellular response to starvation / vesicle-mediated transport / spliceosomal complex / G protein-coupled receptor binding / AUF1 (hnRNP D0) binds and destabilizes mRNA / Late endosomal microautophagy / mRNA splicing, via spliceosome / terminal bouton / protein-macromolecule adaptor activity / regulation of protein stability / Chaperone Mediated Autophagy / melanosome / unfolded protein binding / Clathrin-mediated endocytosis / protein folding / ribonucleoprotein complex / late endosome / MHC class II protein complex binding / protein refolding / lysosomal membrane / chaperone binding / secretory granule lumen / Interleukin-4 and Interleukin-13 signaling / blood microparticle / ficolin-1-rich granule lumen / lysosome / regulation of cell cycle / cadherin binding / glutamatergic synapse / focal adhesion / dendrite / negative regulation of DNA-templated transcription / ubiquitin protein ligase binding / nucleolus / Neutrophil degranulation / ATP hydrolysis activity / perinuclear region of cytoplasm / enzyme binding / RNA binding / extracellular space / extracellular exosome / extracellular region / membrane / nucleoplasm / ATP binding / plasma membrane / nucleus / cytosol / cytoplasm
Similarity search - Function
Defensin A-like - #30 / Heat shock hsp70 proteins family signature 1. / Heat shock hsp70 proteins family signature 2. / Heat shock hsp70 proteins family signature 3. / Heat shock protein 70, conserved site / Heat shock protein 70kD, peptide-binding domain superfamily / Defensin A-like / Heat shock protein 70 family / Hsp70 protein / Heat shock protein 70kD, C-terminal domain superfamily ...Defensin A-like - #30 / Heat shock hsp70 proteins family signature 1. / Heat shock hsp70 proteins family signature 2. / Heat shock hsp70 proteins family signature 3. / Heat shock protein 70, conserved site / Heat shock protein 70kD, peptide-binding domain superfamily / Defensin A-like / Heat shock protein 70 family / Hsp70 protein / Heat shock protein 70kD, C-terminal domain superfamily / ATPase, substrate binding domain, subdomain 4 / Actin; Chain A, domain 4 / ATPase, nucleotide binding domain / ATPase, nucleotide binding domain / Nucleotidyltransferase; domain 5 / Alpha-Beta Complex / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
PHOSPHATE ION / Heat shock cognate 71 kDa protein
Similarity search - Component
|Biological species||Homo sapiens (human)|
|Method||X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.86 Å|
|Citation||Journal: Biochimie / Year: 2015|
Title: New crystal structures of HSC-70 ATP binding domain confirm the role of individual binding pockets and suggest a new method of inhibition.
Authors: Zhang, Z. / Cellitti, J. / Teriete, P. / Pellecchia, M. / Stec, B.
|Structure viewer||Molecule: |
Downloads & links
A: Heat shock cognate 71 kDa protein
B: Heat shock cognate 71 kDa protein
A: Heat shock cognate 71 kDa protein
B: Heat shock cognate 71 kDa protein
-Protein , 1 types, 2 molecules A
Mass: 42165.648 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: HSC70, HSP73, HSPA10, HSPA8 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: P11142
-Non-polymers , 5 types, 827 molecules
|#3: Chemical||#4: Chemical||#5: Chemical|
|#6: Water|| ChemComp-HOH / |
|Sequence details||THE SEQUENCE MATCHES WITH ISOFORM 2 OF UNIPROT ENTRY P11142 WITH IDENTIFIER|
|Experiment||Method: X-RAY DIFFRACTION / Number of used crystals: 1|
|Crystal||Density Matthews: 2.72 Å3/Da / Density % sol: 54.84 %|
|Crystal grow||Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 8.5 |
Details: 0.1 M KCl in Tris, 30%PEG 400, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|Diffraction||Mean temperature: 100 K|
|Diffraction source||Source: ROTATING ANODE / Type: RIGAKU FR-D / Wavelength: 1.54 Å|
|Detector||Type: RIGAKU RAXIS HTC / Detector: IMAGE PLATE / Date: Aug 23, 2007 / Details: Osmic|
|Radiation||Monochromator: Osmic / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray|
|Radiation wavelength||Wavelength: 1.54 Å / Relative weight: 1|
|Reflection||Resolution: 1.86→48 Å / Num. all: 62313 / Num. obs: 62313 / % possible obs: 89 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 2.7 % / Biso Wilson estimate: 28 Å2 / Rmerge(I) obs: 0.083 / Net I/σ(I): 10.1|
|Reflection shell||Resolution: 1.86→1.9 Å / Rmerge(I) obs: 0.51 / Mean I/σ(I) obs: 2.2 / % possible all: 77|
|Refinement||Method to determine structure: MOLECULAR REPLACEMENT|
Starting model: PDB entry 2bup
Resolution: 1.86→48 Å / Cor.coef. Fo:Fc: 0.949 / Cor.coef. Fo:Fc free: 0.915 / SU B: 3.486 / SU ML: 0.105 / Cross valid method: THROUGHOUT / ESU R: 0.166 / ESU R Free: 0.162 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
|Solvent computation||Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK|
|Displacement parameters||Biso mean: 21.868 Å2|
|Refinement step||Cycle: LAST / Resolution: 1.86→48 Å|
|Refine LS restraints|
|LS refinement shell||Resolution: 1.86→1.91 Å / Total num. of bins used: 20 |
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