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- PDB-4h37: Crystal structure of a voltage-gated K+ channel pore domain in a ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4h37 | ||||||
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Title | Crystal structure of a voltage-gated K+ channel pore domain in a closed state in lipid membranes | ||||||
![]() | Lmo2059 protein | ||||||
![]() | MEMBRANE PROTEIN / MODULE / PORE MODULE / ION CHANNEL / MEMBRANE | ||||||
Function / homology | ![]() voltage-gated potassium channel activity / potassium ion transmembrane transport / membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Santos, J.S. / Asmar-Rovira, G.A. / Han, G.W. / Liu, W. / Syeda, R. / Cherezov, V. / Baker, K.A. / Stevens, R.C. / Montal, M. | ||||||
![]() | ![]() Title: Crystal Structure of a Voltage-gated K+ Channel Pore Module in a Closed State in Lipid Membranes. Authors: Santos, J.S. / Asmar-Rovira, G.A. / Han, G.W. / Liu, W. / Syeda, R. / Cherezov, V. / Baker, K.A. / Stevens, R.C. / Montal, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 82.6 KB | Display | ![]() |
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PDB format | ![]() | 62 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 447.5 KB | Display | ![]() |
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Full document | ![]() | 448.6 KB | Display | |
Data in XML | ![]() | 7.8 KB | Display | |
Data in CIF | ![]() | 9.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4h33C ![]() 1orqS ![]() 2a79S ![]() 2atkS ![]() 3effS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 15561.433 Da / Num. of mol.: 2 Fragment: KVLM PORE MODULE, TRUNCATED C-TERMINUS (UNP RESIDUES 98-233) Mutation: A98C Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-K / |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.02 Å3/Da / Density % sol: 39.06 % |
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Crystal grow | Temperature: 293 K / pH: 6.25 Details: 25% (v/v) PEG-MME-550, 0.35 M sodium nitrate, 50 mM ADA, 50 mM sodium malonate pH 7.0, Lipidic Cubic Phase (LCP), temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Mar 18, 2011 |
Radiation | Monochromator: DOUBLE CRYSTAL MONOCHROMATOR / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.033 Å / Relative weight: 1 |
Reflection | Resolution: 3.35→32.6 Å / Num. obs: 3736 / % possible obs: 95.2 % / Redundancy: 5.8 % / Biso Wilson estimate: 101.9 Å2 / Rmerge(I) obs: 0.116 / Net I/σ(I): 15.8 |
Reflection shell | Resolution: 3.35→3.47 Å / Redundancy: 4.8 % / Rmerge(I) obs: 0.66 / Mean I/σ(I) obs: 1.1 / % possible all: 86 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 3EFF, 2ATK , 2A79 AND 1ORQ Resolution: 3.35→32.6 Å / Cor.coef. Fo:Fc: 0.926 / Cor.coef. Fo:Fc free: 0.919 / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters | Biso mean: 192.51 Å2
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Refine analyze | Luzzati coordinate error obs: 1.95 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.35→32.6 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.35→3.75 Å / Total num. of bins used: 5
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Refinement TLS params. | T22: -0.6079 Å2 / Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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