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- PDB-4h33: Crystal structure of a voltage-gated K+ channel pore module in a ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4h33 | ||||||
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Title | Crystal structure of a voltage-gated K+ channel pore module in a closed state in lipid membranes, tetragonal crystal form | ||||||
![]() | Lmo2059 protein | ||||||
![]() | MEMBRANE PROTEIN / BILAYERS / KVLM / LIPIDIC CUBIC PHASE (LCP) / PORE MODULE / ION CHANNEL | ||||||
Function / homology | ![]() voltage-gated potassium channel activity / potassium ion transmembrane transport / membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Santos, J.S. / Asmar-Rovira, G.A. / Han, G.W. / Liu, W. / Syeda, R. / Cherezov, V. / Baker, K.A. / Stevens, R.C. / Montal, M. | ||||||
![]() | ![]() Title: Crystal Structure of a Voltage-gated K+ Channel Pore Module in a Closed State in Lipid Membranes. Authors: Santos, J.S. / Asmar-Rovira, G.A. / Han, G.W. / Liu, W. / Syeda, R. / Cherezov, V. / Baker, K.A. / Stevens, R.C. / Montal, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 52.5 KB | Display | ![]() |
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PDB format | ![]() | 36.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 974.3 KB | Display | ![]() |
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Full document | ![]() | 987.4 KB | Display | |
Data in XML | ![]() | 4.6 KB | Display | |
Data in CIF | ![]() | 5.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4h37C ![]() 1orqS ![]() 2a79S ![]() 2atkS ![]() 3effS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 15561.433 Da / Num. of mol.: 1 Fragment: KVLM PORE MODULE, TRUNCATED C-TERMINUS (UNP RESIDUES 98-233) Mutation: A98C Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||
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#2: Chemical | #3: Chemical | ChemComp-OLC / ( |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.47 Å3/Da / Density % sol: 50.3 % |
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Crystal grow | Temperature: 293 K / pH: 7 Details: 26-30% (v/v) PEG-MME-550, 0.5 M to 0.6 M ammonium sulfate, and 50 mM ADA, Lipidic Cubic Phase (LCP) , pH 7.0, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Mar 18, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.033 Å / Relative weight: 1 |
Reflection | Resolution: 3.1→50 Å / Num. obs: 2994 / % possible obs: 94.4 % / Redundancy: 7.1 % / Biso Wilson estimate: 80.28 Å2 / Rmerge(I) obs: 0.132 / Net I/σ(I): 18.6 |
Reflection shell | Resolution: 3.1→3.21 Å / Redundancy: 5.5 % / Rmerge(I) obs: 0.92 / Mean I/σ(I) obs: 2.45 / % possible all: 75 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 3EFF, 2ATK, 2A79 and 1ORQ Resolution: 3.1→16.4 Å / Cor.coef. Fo:Fc: 0.8555 / Cor.coef. Fo:Fc free: 0.7825 / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters | Biso mean: 114.2 Å2
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Refine analyze | Luzzati coordinate error obs: 1.248 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.1→16.4 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.1→3.47 Å / Total num. of bins used: 5
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Refinement TLS params. | Method: refined / Origin x: 15.3567 Å / Origin y: -5.4997 Å / Origin z: 34.4487 Å
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Refinement TLS group |
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