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Open data
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Basic information
Entry | Database: PDB / ID: 4fzo | ||||||
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Title | Crystal Structure of the apo-form uranyl binding protein | ||||||
![]() | uranyl binding protein | ||||||
![]() | UNKNOWN FUNCTION / uranyl binding / uranyl | ||||||
Function / homology | Domain of unknown function DUF357 / AF1782-like superfamily / Protein of unknown function (DUF357) / AF1782-like / Substrate Binding Domain Of Dnak; Chain:A; Domain 2 / Up-down Bundle / Mainly Alpha / DUF357 domain-containing protein![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | He, C. / Zhou, L. / Zhang, L. | ||||||
![]() | ![]() Title: A protein engineered to bind uranyl selectively and with femtomolar affinity. Authors: Zhou, L. / Bosscher, M. / Zhang, C. / Ozcubukcu, S. / Zhang, L. / Zhang, W. / Li, C.J. / Liu, J. / Jensen, M.P. / Lai, L. / He, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 48.1 KB | Display | ![]() |
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PDB format | ![]() | 35.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 429.9 KB | Display | ![]() |
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Full document | ![]() | 431.6 KB | Display | |
Data in XML | ![]() | 10.1 KB | Display | |
Data in CIF | ![]() | 14.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 9542.686 Da / Num. of mol.: 2 / Mutation: L13D, D17E, H64E, L67T Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H Gene: MTH_1690 / Plasmid: pMCSG19 / Production host: ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.78 Å3/Da / Density % sol: 31.07 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 4.6 Details: PEG 3350, Tacsimate 4.0, sodium acetate, pH 4.6, vapor diffusion, hanging drop, temperature 289K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jun 13, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97918 Å / Relative weight: 1 |
Reflection | Resolution: 1.3→38.2 Å / Num. all: 34522 / Num. obs: 34522 / % possible obs: 15.1 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 1 / Biso Wilson estimate: 7.69 Å2 |
Reflection shell | Resolution: 1.3→1.35 Å / % possible all: 89.2 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 12.305 Å2
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Refinement step | Cycle: LAST / Resolution: 1.3→38.193 Å
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Refine LS restraints |
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LS refinement shell |
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