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Open data
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Basic information
Entry | Database: PDB / ID: 4fiv | ||||||
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Title | FIV PROTEASE COMPLEXED WITH AN INHIBITOR LP-130 | ||||||
![]() | FELINE IMMUNODEFICIENCY VIRUS PROTEASE | ||||||
![]() | ASPARTIC PROTEASE / FIV / RETROPEPSIN / RETROVIRUS / CAT | ||||||
Function / homology | ![]() dUTP catabolic process / dUMP biosynthetic process / dUTP diphosphatase / dUTP diphosphatase activity / Hydrolases; Acting on peptide bonds (peptidases); Aspartic endopeptidases / retroviral ribonuclease H / exoribonuclease H / exoribonuclease H activity / DNA integration / viral genome integration into host DNA ...dUTP catabolic process / dUMP biosynthetic process / dUTP diphosphatase / dUTP diphosphatase activity / Hydrolases; Acting on peptide bonds (peptidases); Aspartic endopeptidases / retroviral ribonuclease H / exoribonuclease H / exoribonuclease H activity / DNA integration / viral genome integration into host DNA / RNA-directed DNA polymerase / establishment of integrated proviral latency / RNA-directed DNA polymerase activity / RNA-DNA hybrid ribonuclease activity / Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases / DNA recombination / Hydrolases; Acting on ester bonds / aspartic-type endopeptidase activity / symbiont entry into host cell / magnesium ion binding / proteolysis / DNA binding / zinc ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Kervinen, J. / Lubkowski, J. / Zdanov, A. / Wlodawer, A. / Gustchina, A. | ||||||
![]() | ![]() Title: Toward a universal inhibitor of retroviral proteases: comparative analysis of the interactions of LP-130 complexed with proteases from HIV-1, FIV, and EIAV. Authors: Kervinen, J. / Lubkowski, J. / Zdanov, A. / Bhatt, D. / Dunn, B.M. / Hui, K.Y. / Powell, D.J. / Kay, J. / Wlodawer, A. / Gustchina, A. | ||||||
History |
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Remark 650 | HELIX DETERMINATION METHOD: TAKEN FROM RELEASED PDB ENTRY 1FIV | ||||||
Remark 700 | SHEET DETERMINATION METHOD: TAKEN FROM RELEASED PDB ENTRY 1FIV |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 41 KB | Display | ![]() |
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PDB format | ![]() | 27 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 732.2 KB | Display | ![]() |
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Full document | ![]() | 737.4 KB | Display | |
Data in XML | ![]() | 8.9 KB | Display | |
Data in CIF | ![]() | 11.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1odyC ![]() 2fmbC ![]() 1fivS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Details | THE AUTHORS NOTE THAT THIS MOLECULE IS ACTIVE AS DIMER |
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Components
#1: Protein | Mass: 12842.825 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: P16088, UniProt: Q66972*PLUS, HIV-1 retropepsin |
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#2: Chemical | ChemComp-LP1 / |
#3: Water | ChemComp-HOH / |
Nonpolymer details | THE INHIBITOR LP-130 (1NI 201) IS TWO-FOLD DISORDERED, SO THE TWO ORIENTATIONS FALL ON TOP OF EACH ...THE INHIBITOR LP-130 (1NI 201) IS TWO-FOLD DISORDERED |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.15 Å3/Da / Density % sol: 43 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.2 / Details: pH 7.2 | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Sep 2, 1994 / Details: MIRRORS |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→40 Å / Num. obs: 9993 / % possible obs: 92.3 % / Observed criterion σ(I): 0 / Redundancy: 3.5 % / Rmerge(I) obs: 0.092 |
Reflection shell | Resolution: 1.8→1.83 Å / Redundancy: 3.4 % / Rmerge(I) obs: 0.273 / % possible all: 85.4 |
Reflection shell | *PLUS % possible obs: 85.4 % |
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Processing
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Refinement | Method to determine structure: RIGID BODY REFINEMENT Starting model: PDB ENTRY 1FIV Resolution: 1.8→10 Å / σ(F): 3 /
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Refinement step | Cycle: LAST / Resolution: 1.8→10 Å
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Refine LS restraints |
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Software | *PLUS Name: PROFFT / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.149 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |