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Yorodumi- PDB-1brs: PROTEIN-PROTEIN RECOGNITION: CRYSTAL STRUCTURAL ANALYSIS OF A BAR... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1brs | ||||||
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Title | PROTEIN-PROTEIN RECOGNITION: CRYSTAL STRUCTURAL ANALYSIS OF A BARNASE-BARSTAR COMPLEX AT 2.0-A RESOLUTION | ||||||
Components |
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Keywords | ENDONUCLEASE | ||||||
Function / homology | Function and homology information Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters / RNA endonuclease activity / RNA binding / extracellular region / cytoplasm Similarity search - Function | ||||||
Biological species | Bacillus amyloliquefaciens (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2 Å | ||||||
Authors | Buckle, A.M. / Schreiber, G. / Fersht, A.R. | ||||||
Citation | Journal: Biochemistry / Year: 1994 Title: Protein-protein recognition: crystal structural analysis of a barnase-barstar complex at 2.0-A resolution. Authors: Buckle, A.M. / Schreiber, G. / Fersht, A.R. #1: Journal: Structure / Year: 1994 Title: Stability and Function: Two Constraints in the Evolution of Barstar and Other Proteins Authors: Schreiber, G. / Buckle, A.M. / Fersht, A.R. #2: Journal: Structure / Year: 1993 Title: Recognition between a Bacterial Ribonuclease, Barnase, and its Natural Inhibitor, Barstar Authors: Guillet, V. / Lapthorn, A. / Hartley, R.W. / Mauguen, Y. #3: Journal: Biochemistry / Year: 1993 Title: Interaction of Barnase with its Polypeptide Inhibitor Barstar Studied by Protein Engineering Authors: Schreiber, G. / Fersht, A.R. #4: Journal: Nature / Year: 1982 Title: Molecular Structures of a New Family of Ribonucleases Authors: Mauguen, Y. / Hartley, R.W. / Dodson, E.J. / Dodson, G.G. / Bricogne, G. / Chothia, C. / Jack, A. | ||||||
History |
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Remark 700 | SHEET IN *SHEET* AND *TURN* RECORDS BELOW, *BN* REFERS TO BARNASE AND *BS* REFERS TO BARSTAR. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1brs.cif.gz | 135.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1brs.ent.gz | 107.2 KB | Display | PDB format |
PDBx/mmJSON format | 1brs.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/br/1brs ftp://data.pdbj.org/pub/pdb/validation_reports/br/1brs | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO D 48 / 2: CIS PROLINE - PRO E 48 / 3: CIS PROLINE - PRO F 48 | |||||||||
Components on special symmetry positions |
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-Components
#1: Protein | Mass: 12398.721 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus amyloliquefaciens (bacteria) / Production host: Escherichia coli (E. coli) / Strain (production host): BL21-(PLYSE) (PML2BS) CELLS References: UniProt: P00648, Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters #2: Protein | Mass: 10157.412 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus amyloliquefaciens (bacteria) / Production host: Escherichia coli (E. coli) / Strain (production host): BL21-(PLYSE) (PML2BS) CELLS / References: UniProt: P11540 #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.71 Å3/Da / Density % sol: 54.59 % | |||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 23 ℃ / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2 Å / Num. obs: 43964 / % possible obs: 89.7 % / Observed criterion σ(F): 37.1 / Num. measured all: 142093 / Rmerge(I) obs: 0.09 |
-Processing
Software | Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Resolution: 2→7 Å / σ(F): 0 /
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Refinement step | Cycle: LAST / Resolution: 2→7 Å
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Refine LS restraints |
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Refinement | *PLUS Rfactor obs: 0.173 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |