+Open data
-Basic information
Entry | Database: PDB / ID: 3d20 | ||||||
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Title | Crystal structure of HIV-1 mutant I54V and inhibitor DARUNAVIA | ||||||
Components | HIV-1 Protease | ||||||
Keywords | HYDROLASE / DRUG RESISTANCE / HIV-1 / I54V / FLAP MUTANT / AIDS / Aspartyl protease / Capsid maturation / Capsid protein / DNA integration / DNA recombination / DNA-directed DNA polymerase / Endonuclease / Lipoprotein / Magnesium / Membrane / Metal-binding / Multifunctional enzyme / Myristate / Nuclease / Nucleotidyltransferase / Nucleus / Phosphoprotein / Protease / RNA-binding / RNA-directed DNA polymerase / Transferase / Viral nucleoprotein / Virion / Zinc-finger | ||||||
Function / homology | Function and homology information HIV-1 retropepsin / symbiont-mediated activation of host apoptosis / retroviral ribonuclease H / exoribonuclease H / exoribonuclease H activity / host multivesicular body / DNA integration / viral genome integration into host DNA / RNA-directed DNA polymerase / establishment of integrated proviral latency ...HIV-1 retropepsin / symbiont-mediated activation of host apoptosis / retroviral ribonuclease H / exoribonuclease H / exoribonuclease H activity / host multivesicular body / DNA integration / viral genome integration into host DNA / RNA-directed DNA polymerase / establishment of integrated proviral latency / viral penetration into host nucleus / symbiont-mediated suppression of host gene expression / RNA stem-loop binding / RNA-directed DNA polymerase activity / RNA-DNA hybrid ribonuclease activity / Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases / host cell / viral nucleocapsid / DNA recombination / DNA-directed DNA polymerase / Hydrolases; Acting on ester bonds / aspartic-type endopeptidase activity / DNA-directed DNA polymerase activity / symbiont entry into host cell / viral translational frameshifting / lipid binding / host cell nucleus / host cell plasma membrane / structural molecule activity / virion membrane / proteolysis / DNA binding / zinc ion binding / membrane Similarity search - Function | ||||||
Biological species | Human immunodeficiency virus type 1 | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.05 Å | ||||||
Authors | Liu, F. / Kovalesky, A.Y. / Tie, Y. / Ghosh, A.K. / Harrison, R.W. / Weber, I.T. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2008 Title: Effect of flap mutations on structure of HIV-1 protease and inhibition by saquinavir and darunavir. Authors: Liu, F. / Kovalevsky, A.Y. / Tie, Y. / Ghosh, A.K. / Harrison, R.W. / Weber, I.T. #1: Journal: J.Mol.Biol. / Year: 2006 Title: Ultra-High Resolution Crystal Structure of HIV-1 Protease Mutant Reveals Two Binding Sites for Clinical Inhibitor Tmc114 Authors: Kovalevsky, A.Y. / Liu, F. / Leshchenko, S. / Ghosh, A.K. / Harrison, R.W. / Weber, I.T. #2: Journal: J.Mol.Biol. / Year: 2005 Title: Kinetic, Stability, and Structural Changes in High-Resolution Crystal Structures of HIV-1 Protease with Drug-Resistant Mutations L24I, I50V, and G73S. Authors: Liu, F. / Boross, P.I. / Wang, Y.F. / Tozser, J. / Louis, J.M. / Harrison, R.W. / Weber, I.T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3d20.cif.gz | 108.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3d20.ent.gz | 82.7 KB | Display | PDB format |
PDBx/mmJSON format | 3d20.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 3d20_validation.pdf.gz | 1.1 MB | Display | wwPDB validaton report |
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Full document | 3d20_full_validation.pdf.gz | 1.1 MB | Display | |
Data in XML | 3d20_validation.xml.gz | 13.3 KB | Display | |
Data in CIF | 3d20_validation.cif.gz | 18.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/d2/3d20 ftp://data.pdbj.org/pub/pdb/validation_reports/d2/3d20 | HTTPS FTP |
-Related structure data
Related structure data | 3cywC 3cyxC 3d1xC 3d1yC 3d1zC 1dazS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 10726.649 Da / Num. of mol.: 2 / Mutation: Q7K, L33I, L63I, C67A, C95A, I54V Source method: isolated from a genetically manipulated source Source: (gene. exp.) Human immunodeficiency virus type 1 / Gene: gag-pol / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: P04587, HIV-1 retropepsin #2: Chemical | ChemComp-NA / | #3: Chemical | #4: Chemical | ChemComp-017 / ( | #5: Water | ChemComp-HOH / | Sequence details | MUTATIONS Q7K, L33I, L63I, C67A, C95A, HAVE BEEN MADE TO STABILIZE THE PROTEASE FROM ...MUTATIONS Q7K, L33I, L63I, C67A, C95A, HAVE BEEN MADE TO STABILIZE THE PROTEASE FROM AUTOPROTEO | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.73 Å3/Da / Density % sol: 54.9 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 6 Details: 0.2M SODIUM ACETATE, PHOSPHATE BUFFER, 30% AMmONIUM SULFATE, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 95 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Jun 12, 2005 |
Radiation | Monochromator: SI / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.05→50 Å / Num. all: 100265 / Num. obs: 81638 / % possible obs: 91.2 % / Observed criterion σ(F): 4 / Observed criterion σ(I): 2 / Redundancy: 6 % / Rmerge(I) obs: 0.108 / Net I/σ(I): 17.8 |
Reflection shell | Resolution: 1.05→1.09 Å / Redundancy: 2 % / Rmerge(I) obs: 0.42 / Mean I/σ(I) obs: 1.8 / % possible all: 91.2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 1DAZ Resolution: 1.05→10 Å / Num. parameters: 17193 / Num. restraintsaints: 22317 / Cross valid method: FREE R / σ(F): 0 / Stereochemistry target values: ENGH AND HUBER / Details: ANISOTROPIC REFINEMENT REDUCED FREE R (NO CUTOFF)
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Refine analyze | Num. disordered residues: 27 / Occupancy sum hydrogen: 1614 / Occupancy sum non hydrogen: 1762.6 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.05→10 Å
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Refine LS restraints |
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