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Open data
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Basic information
Entry | Database: PDB / ID: 4e7u | ||||||
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Title | The structure of T3R3 bovine insulin | ||||||
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Function / homology | ![]() positive regulation of blood circulation / estradiol secretion / negative regulation of lactation / glucose import in response to insulin stimulus / positive regulation of lactation / positive regulation of cell maturation / response to L-arginine / response to butyrate / positive regulation of mammary gland epithelial cell proliferation / negative regulation of appetite ...positive regulation of blood circulation / estradiol secretion / negative regulation of lactation / glucose import in response to insulin stimulus / positive regulation of lactation / positive regulation of cell maturation / response to L-arginine / response to butyrate / positive regulation of mammary gland epithelial cell proliferation / negative regulation of appetite / ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Harris, P. / Frankaer, C.G. / Knudsen, M.V. | ||||||
![]() | ![]() Title: The structures of T(6), T(3)R(3) and R(6) bovine insulin: combining X-ray diffraction and absorption spectroscopy. Authors: Frankar, C.G. / Knudsen, M.V. / Noren, K. / Nazarenko, E. / Stahl, K. / Harris, P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 68.8 KB | Display | ![]() |
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PDB format | ![]() | 56.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 312.1 KB | Display | ![]() |
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Full document | ![]() | 312.9 KB | Display | |
Data in XML | ![]() | 7.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4e7tC ![]() 4e7vC ![]() 1benS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein/peptide | Mass: 2339.645 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() ![]() #2: Protein/peptide | Mass: 3403.927 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() ![]() #3: Chemical | #4: Chemical | ChemComp-SCN / | ![]() #5: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.96 Å3/Da / Density % sol: 37.11 % |
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Crystal grow![]() | Temperature: 298 K / Method: batch method / pH: 6.4 Details: 6.5mg/ml bovine insulin, 7mM zinc acetate, 0.05M sodium citrate, 15% acetone, 0.2M KSCN, pH 6.4, BATCH METHOD, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Oct 28, 2010 |
Radiation | Monochromator: Bent Si (111) crystal, horizontally focusing / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.23→21.27 Å / Num. all: 23527 / Num. obs: 23527 / % possible obs: 93 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 1BEN Resolution: 1.3→21.27 Å / SU ML: 0.16 / σ(F): 2 / Phase error: 14.03 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.05 Å / VDW probe radii: 0.3 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 66.077 Å2 / ksol: 0.539 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.3→21.27 Å
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Refine LS restraints |
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LS refinement shell |
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