+Open data
-Basic information
Entry | Database: PDB / ID: 4.0E+61 | ||||||
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Title | Crystal structure of the EB1-like motif of Bim1p | ||||||
Components | Protein BIM1 | ||||||
Keywords | CELL CYCLE / EB1-like motif / coiled-coil / spindle orientation / mitosis / Kar9p / phosphorylation / mitotic spindle / microtubules | ||||||
Function / homology | Function and homology information protein localization to microtubule plus-end / mitotic spindle orientation checkpoint signaling / 2-micrometer plasmid partitioning / protein localization to microtubule / nuclear migration along microtubule / microtubule plus-end / microtubule nucleation / negative regulation of microtubule depolymerization / microtubule plus-end binding / microtubule organizing center ...protein localization to microtubule plus-end / mitotic spindle orientation checkpoint signaling / 2-micrometer plasmid partitioning / protein localization to microtubule / nuclear migration along microtubule / microtubule plus-end / microtubule nucleation / negative regulation of microtubule depolymerization / microtubule plus-end binding / microtubule organizing center / microtubule depolymerization / mitotic sister chromatid cohesion / cytoplasmic microtubule / regulation of microtubule polymerization or depolymerization / spindle assembly / spindle midzone / positive regulation of microtubule polymerization / mitotic spindle / spindle pole / cell division Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.45 Å | ||||||
Authors | Huels, D. / Storchova, Z. / Niessing, D. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2012 Title: Post-translational Modifications Regulate Assembly of Early Spindle Orientation Complex in Yeast. Authors: Huls, D. / Storchova, Z. / Niessing, D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4e61.cif.gz | 44.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4e61.ent.gz | 32.4 KB | Display | PDB format |
PDBx/mmJSON format | 4e61.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 4e61_validation.pdf.gz | 432.8 KB | Display | wwPDB validaton report |
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Full document | 4e61_full_validation.pdf.gz | 435 KB | Display | |
Data in XML | 4e61_validation.xml.gz | 7.9 KB | Display | |
Data in CIF | 4e61_validation.cif.gz | 9.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/e6/4e61 ftp://data.pdbj.org/pub/pdb/validation_reports/e6/4e61 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 11808.162 Da / Num. of mol.: 2 Fragment: C-terminal domain, EB1-like motif (UNP residues 182-282) Source method: isolated from a genetically manipulated source Details: Sequence GPLGS at the N-terminus is derived from expression vector Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Strain: BY4741 Mata / Gene: BIM1, YER016W / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) / References: UniProt: P40013 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.53 Å3/Da / Density % sol: 51.42 % |
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Crystal grow | Temperature: 294.15 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 0.1M Bis-Tris pH 8.0, 20% PEG 5000MME, 40% gamma-butyrlactone, VAPOR DIFFUSION, HANGING DROP, temperature 294.15K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID29 / Wavelength: 1.25433 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Sep 26, 2009 |
Radiation | Monochromator: Si111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.25433 Å / Relative weight: 1 |
Reflection | Resolution: 2.45→20 Å / Num. all: 8938 / Num. obs: 8832 / % possible obs: 98.81 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 13.12 |
Reflection shell | Resolution: 2.45→2.59 Å / % possible all: 98.29 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: Not fully refined model after structure determination by SAD with potassium tetranitroplatinate(II)-soaked crystals. Resolution: 2.45→20 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.45→20 Å
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Refine LS restraints |
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