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- PDB-4dgv: Structure of the Hepatitis C virus envelope glycoprotein E2 antig... -

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Basic information

Entry
Database: PDB / ID: 4dgv
TitleStructure of the Hepatitis C virus envelope glycoprotein E2 antigenic region 412-423 bound to the broadly neutralizing antibody HCV1, P2(1) form
Components
  • E2 peptide
  • HCV1 Heavy Chain
  • HCV1 Light Chain
KeywordsIMMUNE SYSTEM/VIRAL PROTEIN / Immunoglobulin Fold / IMMUNE SYSTEM-VIRAL PROTEIN complex
Function / homology
Function and homology information


hepacivirin / host cell mitochondrial membrane / host cell lipid droplet / modulation by virus of host G1/S transition checkpoint / transformation of host cell by virus / host cell cytosol / lipid droplet / suppression by virus of host JAK-STAT cascade via inhibition of STAT1 activity / suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / serine-type peptidase activity ...hepacivirin / host cell mitochondrial membrane / host cell lipid droplet / modulation by virus of host G1/S transition checkpoint / transformation of host cell by virus / host cell cytosol / lipid droplet / suppression by virus of host JAK-STAT cascade via inhibition of STAT1 activity / suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / serine-type peptidase activity / host cell endoplasmic reticulum membrane / SH3 domain binding / integral to membrane of host cell / pore formation by virus in membrane of host cell / suppression by virus of host TRAF activity / protein complex oligomerization / nucleoside-triphosphate phosphatase / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases / ion channel activity / host cell perinuclear region of cytoplasm / clathrin-dependent endocytosis of virus by host cell / viral nucleocapsid / RNA helicase activity / protease binding / RNA helicase / induction by virus of host autophagy / RNA-directed RNA polymerase / cysteine-type endopeptidase activity / viral RNA genome replication / RNA-directed 5'-3' RNA polymerase activity / suppression by virus of host type I interferon-mediated signaling pathway / virion attachment to host cell / fusion of virus membrane with host endosome membrane / host cell cytoplasm / viral envelope / : / host cell nucleus / serine-type endopeptidase activity / host cell plasma membrane / virion membrane / structural molecule activity / RNA binding / zinc ion binding / membrane / integral component of membrane / ATP binding / identical protein binding
Similarity search - Function
Hepatitis C virus NS5A, 1B domain superfamily / Hepatitis C virus non-structural protein NS2, C-terminal domain / Hepatitis C virus non-structural protein NS2, N-terminal domain / HCV NS5a protein C-terminal region / Hepatitus C virus, NS5a, C-terminal / Hepatitis C virus non-structural protein NS4b / Hepatitis C virus non-structural protein NS4b / Hepatitis C virus core protein, N-terminal / Hepatitis C virus capsid protein / NS5A domain 1a ...Hepatitis C virus NS5A, 1B domain superfamily / Hepatitis C virus non-structural protein NS2, C-terminal domain / Hepatitis C virus non-structural protein NS2, N-terminal domain / HCV NS5a protein C-terminal region / Hepatitus C virus, NS5a, C-terminal / Hepatitis C virus non-structural protein NS4b / Hepatitis C virus non-structural protein NS4b / Hepatitis C virus core protein, N-terminal / Hepatitis C virus capsid protein / NS5A domain 1a / Hepatitis C virus non-structural 5a, 1B domain / Hepatitis C virus non-structural 5a zinc finger domain / Hepatitis C virus non-structural protein NS2 / Hepatitis C virus non-structural 5a protein membrane anchor / Hepatitis C virus non-structural 5a protein / Hepatitis C virus non-structural 5a domain 1b / Hepatitis C virus non-structural protein NS2 / Hepacivirus nonstructural protein 2 (NS2) protease domain profile. / NS5A domain 1a superfamily / Hepatitis C virus non-structural protein NS4a / Hepatitis C virus non-structural protein NS4a / Hepatitis C virus core protein, C-terminal / Hepatitis C virus core protein / Hepatitis C virus non-structural protein E2/NS1 / Hepatitis C virus non-structural protein E2/NS1 / Hepatitis C virus envelope glycoprotein E1 / Hepatitis C virus envelope glycoprotein E1 / Hepacivirus/Pegivirus NS3 protease domain profile. / Peptidase S29, hepatitis C virus NS3 protease / Hepatitis C virus NS3 protease / Viral RNA dependent RNA polymerase / RNA dependent RNA polymerase, hepatitis C virus / DEAD box, Flavivirus / Flavivirus DEAD domain / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / Helicase, C-terminal / DEAD-like helicases superfamily / Helicase superfamily 1/2, ATP-binding domain / RNA-directed RNA polymerase, catalytic domain / RdRp of positive ssRNA viruses catalytic domain profile. / Reverse transcriptase/Diguanylate cyclase domain / Peptidase S1, PA clan, chymotrypsin-like fold / DNA/RNA polymerase superfamily / Peptidase S1, PA clan / Immunoglobulins / Immunoglobulin-like / P-loop containing nucleoside triphosphate hydrolase / Sandwich / Mainly Beta
Similarity search - Domain/homology
: / Genome polyprotein / Envelope glycoprotein E1
Similarity search - Component
Biological speciesHomo sapiens (human)
Hepatitis C virus
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.805 Å
AuthorsKong, L. / Wilson, I.A. / Law, M.
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2012
Title: Structural basis of hepatitis C virus neutralization by broadly neutralizing antibody HCV1.
Authors: Kong, L. / Giang, E. / Robbins, J.B. / Stanfield, R.L. / Burton, D.R. / Wilson, I.A. / Law, M.
History
DepositionJan 26, 2012Deposition site: RCSB / Processing site: RCSB
Revision 1.0May 23, 2012Provider: repository / Type: Initial release
Revision 1.1Jun 6, 2012Group: Database references
Revision 1.2Jun 27, 2012Group: Database references

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
H: HCV1 Heavy Chain
L: HCV1 Light Chain
A: E2 peptide
hetero molecules


Theoretical massNumber of molelcules
Total (without water)49,5935
Polymers49,5313
Non-polymers622
Water5,711317
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area5460 Å2
ΔGint-50 kcal/mol
Surface area20130 Å2
MethodPISA
Unit cell
γ
α
β
Length a, b, c (Å)44.041, 75.301, 60.800
Angle α, β, γ (deg.)90.00, 91.53, 90.00
Int Tables number4
Space group name H-MP1211

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Components

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Protein/peptide , 1 types, 1 molecules A

#3: Protein/peptide E2 peptide


Mass: 1554.710 Da / Num. of mol.: 1 / Fragment: UNP residues 51-62 / Source method: obtained synthetically / Source: (synth.) Hepatitis C virus / References: UniProt: Q9YK84, UniProt: P26663*PLUS

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Antibody , 2 types, 2 molecules HL

#1: Antibody HCV1 Heavy Chain


Mass: 24580.699 Da / Num. of mol.: 1 / Fragment: Fab
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): HEK 293 Freestyle / Production host: Homo sapiens (human)
#2: Antibody HCV1 Light Chain


Mass: 23395.906 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): HEK 293 Freestyle / Production host: Homo sapiens (human)

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Non-polymers , 3 types, 319 molecules

#4: Chemical ChemComp-NA / SODIUM ION


Mass: 22.990 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Na
#5: Chemical ChemComp-K / POTASSIUM ION


Mass: 39.098 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: K
#6: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 317 / Source method: isolated from a natural source / Formula: H2O

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Details

Sequence detailsAN N-TERMINAL ARG RESIDUE HAS BEEN ADDED TO THE E2 PEPTIDE TO AID SOLUBILITY.

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.03 Å3/Da / Density % sol: 39.55 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop
Details: 40 mM potassium dihydrogen phosphate, 20% glycerol, 16% PEG8000, VAPOR DIFFUSION, SITTING DROP, temperature 293K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 23-ID-D / Wavelength: 1.033 Å
DetectorType: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Aug 17, 2011
RadiationMonochromator: Double crystal cryo-cooled Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.033 Å / Relative weight: 1
ReflectionResolution: 1.805→75.301 Å / Num. all: 36325 / Num. obs: 36325 / % possible obs: 99.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 3.6 % / Biso Wilson estimate: 21 Å2 / Rsym value: 0.11 / Net I/σ(I): 9.8
Reflection shellResolution: 1.805→1.84 Å / Redundancy: 3.5 % / Mean I/σ(I) obs: 3 / Num. unique all: 1809 / Rsym value: 0.62 / % possible all: 99.5

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Processing

Software
NameVersionClassification
Blu-Icedata collection
PHASERphasing
PHENIX(phenix.refine: 1.7.2_869)refinement
HKL-2000data reduction
SCALEPACKdata scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 3GIZ
Resolution: 1.805→44.025 Å / SU ML: 0.37 / σ(F): 0 / Phase error: 21.67 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.2139 1813 4.99 %RANDOM
Rwork0.1701 ---
all0.1723 36301 --
obs0.1723 36301 99.39 %-
Solvent computationShrinkage radii: 0.73 Å / VDW probe radii: 1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 43.192 Å2 / ksol: 0.391 e/Å3
Displacement parametersBiso mean: 26.7 Å2
Baniso -1Baniso -2Baniso -3
1-2.3967 Å20 Å20.8052 Å2
2--2.1989 Å2-0 Å2
3----4.4552 Å2
Refinement stepCycle: LAST / Resolution: 1.805→44.025 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms3479 0 2 317 3798
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0073633
X-RAY DIFFRACTIONf_angle_d1.2084947
X-RAY DIFFRACTIONf_dihedral_angle_d12.7151312
X-RAY DIFFRACTIONf_chiral_restr0.083556
X-RAY DIFFRACTIONf_plane_restr0.005638
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.805-1.85410.29041320.22422513X-RAY DIFFRACTION94
1.8541-1.90860.27191450.20662655X-RAY DIFFRACTION100
1.9086-1.97020.22571170.1952638X-RAY DIFFRACTION100
1.9702-2.04070.24241280.19012665X-RAY DIFFRACTION100
2.0407-2.12240.24721360.18012656X-RAY DIFFRACTION100
2.1224-2.2190.20891290.18382669X-RAY DIFFRACTION100
2.219-2.33590.24181310.17452667X-RAY DIFFRACTION100
2.3359-2.48230.23511300.18882671X-RAY DIFFRACTION100
2.4823-2.67390.25721510.19252657X-RAY DIFFRACTION100
2.6739-2.9430.25251700.18622657X-RAY DIFFRACTION100
2.943-3.36870.19331550.17232657X-RAY DIFFRACTION100
3.3687-4.24360.18241510.13742660X-RAY DIFFRACTION100
4.2436-44.03830.171380.14772723X-RAY DIFFRACTION99

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