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Yorodumi- PDB-4dgv: Structure of the Hepatitis C virus envelope glycoprotein E2 antig... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4dgv | ||||||
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Title | Structure of the Hepatitis C virus envelope glycoprotein E2 antigenic region 412-423 bound to the broadly neutralizing antibody HCV1, P2(1) form | ||||||
Components |
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Keywords | IMMUNE SYSTEM/VIRAL PROTEIN / Immunoglobulin Fold / IMMUNE SYSTEM-VIRAL PROTEIN complex | ||||||
Function / homology | Function and homology information hepacivirin / host cell mitochondrial membrane / host cell lipid droplet / symbiont-mediated suppression of host TRAF-mediated signal transduction / transformation of host cell by virus / symbiont-mediated perturbation of host cell cycle G1/S transition checkpoint / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / lipid droplet / SH3 domain binding ...hepacivirin / host cell mitochondrial membrane / host cell lipid droplet / symbiont-mediated suppression of host TRAF-mediated signal transduction / transformation of host cell by virus / symbiont-mediated perturbation of host cell cycle G1/S transition checkpoint / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / lipid droplet / SH3 domain binding / : / nucleoside-triphosphate phosphatase / protein complex oligomerization / monoatomic ion channel activity / clathrin-dependent endocytosis of virus by host cell / viral nucleocapsid / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases / RNA helicase activity / host cell endoplasmic reticulum membrane / host cell perinuclear region of cytoplasm / RNA helicase / induction by virus of host autophagy / RNA-directed RNA polymerase / ribonucleoprotein complex / viral RNA genome replication / cysteine-type endopeptidase activity / RNA-dependent RNA polymerase activity / serine-type endopeptidase activity / fusion of virus membrane with host endosome membrane / viral envelope / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / host cell nucleus / structural molecule activity / virion attachment to host cell / host cell plasma membrane / virion membrane / ATP hydrolysis activity / proteolysis / RNA binding / zinc ion binding / ATP binding / membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) Hepatitis C virus | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.805 Å | ||||||
Authors | Kong, L. / Wilson, I.A. / Law, M. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2012 Title: Structural basis of hepatitis C virus neutralization by broadly neutralizing antibody HCV1. Authors: Kong, L. / Giang, E. / Robbins, J.B. / Stanfield, R.L. / Burton, D.R. / Wilson, I.A. / Law, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4dgv.cif.gz | 110.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4dgv.ent.gz | 82.2 KB | Display | PDB format |
PDBx/mmJSON format | 4dgv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/dg/4dgv ftp://data.pdbj.org/pub/pdb/validation_reports/dg/4dgv | HTTPS FTP |
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-Related structure data
Related structure data | 4dgyC 3gizS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein/peptide , 1 types, 1 molecules A
#3: Protein/peptide | Mass: 1554.710 Da / Num. of mol.: 1 / Fragment: UNP residues 51-62 / Source method: obtained synthetically / Source: (synth.) Hepatitis C virus / References: UniProt: Q9YK84, UniProt: P26663*PLUS |
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-Antibody , 2 types, 2 molecules HL
#1: Antibody | Mass: 24580.699 Da / Num. of mol.: 1 / Fragment: Fab Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): HEK 293 Freestyle / Production host: Homo sapiens (human) |
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#2: Antibody | Mass: 23395.906 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): HEK 293 Freestyle / Production host: Homo sapiens (human) |
-Non-polymers , 3 types, 319 molecules
#4: Chemical | ChemComp-NA / |
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#5: Chemical | ChemComp-K / |
#6: Water | ChemComp-HOH / |
-Details
Sequence details | AN N-TERMINAL ARG RESIDUE HAS BEEN ADDED TO THE E2 PEPTIDE TO AID SOLUBILITY |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.03 Å3/Da / Density % sol: 39.55 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 40 mM potassium dihydrogen phosphate, 20% glycerol, 16% PEG8000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 23-ID-D / Wavelength: 1.033 Å |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Aug 17, 2011 |
Radiation | Monochromator: Double crystal cryo-cooled Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.033 Å / Relative weight: 1 |
Reflection | Resolution: 1.805→75.301 Å / Num. all: 36325 / Num. obs: 36325 / % possible obs: 99.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 3.6 % / Biso Wilson estimate: 21 Å2 / Rsym value: 0.11 / Net I/σ(I): 9.8 |
Reflection shell | Resolution: 1.805→1.84 Å / Redundancy: 3.5 % / Mean I/σ(I) obs: 3 / Num. unique all: 1809 / Rsym value: 0.62 / % possible all: 99.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 3GIZ Resolution: 1.805→44.025 Å / SU ML: 0.37 / σ(F): 0 / Phase error: 21.67 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.73 Å / VDW probe radii: 1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 43.192 Å2 / ksol: 0.391 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 26.7 Å2
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Refinement step | Cycle: LAST / Resolution: 1.805→44.025 Å
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Refine LS restraints |
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LS refinement shell |
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