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Open data
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Basic information
Entry | Database: PDB / ID: 4ceh | ||||||
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Title | Crystal structure of AddAB with a forked DNA substrate | ||||||
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![]() | HYDROLASE/DNA / HYDROLASE-DNA COMPLEX / HELICASE-NUCLEASE / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | ||||||
Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() SYNTHETIC CONSTRUCT (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Krajewski, W.W. / Wilkinson, M. / Fu, X. / Cronin, N.B. / Wigley, D. | ||||||
![]() | ![]() Title: Structural Basis for Translocation by Addab Helicase-Nuclease and its Arrest at Chi Sites. Authors: Krajewski, W.W. / Fu, X. / Wilkinson, M. / Cronin, N.B. / Dillingham, M.S. / Wigley, D.B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 497.1 KB | Display | ![]() |
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PDB format | ![]() | 394.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4ceiC ![]() 4cejC ![]() 3u44S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 141218.844 Da / Num. of mol.: 1 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Plasmid: PCOLADUET-1 / Production host: ![]() ![]() ![]() References: UniProt: P23478, ![]() ![]() |
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#2: Protein | Mass: 134769.406 Da / Num. of mol.: 1 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Plasmid: PCOLADUET-1 / Production host: ![]() ![]() ![]() References: UniProt: P23477, ![]() ![]() |
#3: DNA chain | ![]() Mass: 19956.744 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: SYNTHETIC CONSTRUCT / Source: (synth.) SYNTHETIC CONSTRUCT (others) |
#4: Chemical | ChemComp-SF4 / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.99 Å3/Da / Density % sol: 59 % / Description: NONE |
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Crystal grow![]() | Temperature: 285 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 0.1M TRIS-HCL PH 7.5, 15% PEG 4000, 0.8M SODIUM FORMATE, VAPOR DIFFUSION, HANGING DROP, 285K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 8, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 3.24→39.52 Å / Num. obs: 47940 / % possible obs: 98.5 % / Observed criterion σ(I): 2 / Redundancy: 2.9 % / Rmerge(I) obs: 0.05 / Net I/σ(I): 13.2 |
Reflection shell | Resolution: 3.24→3.35 Å / Redundancy: 2.9 % / Rmerge(I) obs: 0.33 / Mean I/σ(I) obs: 2.3 / % possible all: 97.7 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 3U44 Resolution: 3.24→29.939 Å / SU ML: 0.42 / σ(F): 1.97 / Phase error: 32.34 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.24→29.939 Å
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Refine LS restraints |
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LS refinement shell |
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