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- PDB-4cc2: Complex of human Tuba C-terminal SH3 domain with human N-WASP pro... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4cc2 | ||||||
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Title | Complex of human Tuba C-terminal SH3 domain with human N-WASP proline- rich peptide - P212121 | ||||||
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![]() | STRUCTURAL PROTEIN / SRC HOMOLOGY 3 / SH3 DOMAIN / PROLINE-RICH PEPTIDE / ACTIN CYTOSKELETON / CORTICAL TENSION | ||||||
Function / homology | ![]() negative regulation of membrane tubulation / spindle localization / positive regulation of clathrin-dependent endocytosis / negative regulation of lymphocyte migration / NOSTRIN mediated eNOS trafficking / GTPase regulator activity / actin cap / vesicle organization / vesicle budding from membrane / Golgi stack ...negative regulation of membrane tubulation / spindle localization / positive regulation of clathrin-dependent endocytosis / negative regulation of lymphocyte migration / NOSTRIN mediated eNOS trafficking / GTPase regulator activity / actin cap / vesicle organization / vesicle budding from membrane / Golgi stack / vesicle transport along actin filament / protein-containing complex localization / actin polymerization or depolymerization / dendritic spine morphogenesis / Nephrin family interactions / regulation of small GTPase mediated signal transduction / DCC mediated attractive signaling / positive regulation of filopodium assembly / regulation of postsynapse organization / RHOV GTPase cycle / RHOJ GTPase cycle / RHOQ GTPase cycle / CDC42 GTPase cycle / cilium assembly / RHO GTPases Activate WASPs and WAVEs / EPHB-mediated forward signaling / RAC1 GTPase cycle / actin filament polymerization / guanyl-nucleotide exchange factor activity / FCGR3A-mediated phagocytosis / response to bacterium / Regulation of actin dynamics for phagocytic cup formation / regulation of protein localization / endocytic vesicle membrane / cell-cell junction / actin cytoskeleton / presynapse / lamellipodium / Clathrin-mediated endocytosis / actin binding / regulation of cell shape / cytoplasmic vesicle / microtubule binding / protein-containing complex assembly / cytoskeleton / intracellular signal transduction / cell division / glutamatergic synapse / synapse / endoplasmic reticulum membrane / Golgi apparatus / positive regulation of transcription by RNA polymerase II / extracellular exosome / nucleus / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Polle, L. / Rigano, L. / Julian, R. / Ireton, K. / Schubert, W.-D. | ||||||
![]() | ![]() Title: Structural Details of Human Tuba Recruitment by Inlc of Listeria Monocytogenes Elucidate Bacterial Cell-Cell Spreading. Authors: Polle, L. / Rigano, L.A. / Julian, R. / Ireton, K. / Schubert, W. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 79.1 KB | Display | ![]() |
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PDB format | ![]() | 60.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 465.1 KB | Display | ![]() |
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Full document | ![]() | 469.1 KB | Display | |
Data in XML | ![]() | 11.3 KB | Display | |
Data in CIF | ![]() | 15.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4cc3C ![]() 4cc4C ![]() 4cc7C ![]() 1zuuS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 7712.617 Da / Num. of mol.: 2 Fragment: C-TERMINAL SH3 DOMAIN OF HUMAN TUBA, RESIDUES 1513-1577 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein/peptide | Mass: 1077.188 Da / Num. of mol.: 2 / Fragment: N-WASP PROLINE-RICH PEPTIDE, RESIDUES 346-357 / Source method: obtained synthetically / Source: (synth.) ![]() #3: Chemical | ChemComp-GOL / #4: Chemical | ChemComp-CL / #5: Water | ChemComp-HOH / | Sequence details | FIRST TWO RESIDUES, GP, DERIVE FROM FUSION PROTEIN CLEAVAGE SITE | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.81 Å3/Da / Density % sol: 32 % / Description: NONE |
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Crystal grow | pH: 5.5 / Details: 3 M NACL, 0.1 M BIS-TRIS PH 5.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Dec 18, 2011 / Details: KIRKPATRICK-BAEZ PAIR OF BI-MORPH MIRRORS |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 1.55→50 Å / Num. obs: 18318 / % possible obs: 93.4 % / Observed criterion σ(I): -3 / Redundancy: 2.8 % / Rmerge(I) obs: 0.04 / Net I/σ(I): 15.5 |
Reflection shell | Resolution: 1.55→1.64 Å / Redundancy: 2.7 % / Rmerge(I) obs: 0.39 / Mean I/σ(I) obs: 2.7 / % possible all: 95.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1ZUU Resolution: 1.55→40.06 Å / Cor.coef. Fo:Fc: 0.966 / Cor.coef. Fo:Fc free: 0.95 / SU B: 2.624 / SU ML: 0.049 / Cross valid method: THROUGHOUT / ESU R: 0.02 / ESU R Free: 0.021 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES WITH TLS ADDED.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 20.601 Å2
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Refinement step | Cycle: LAST / Resolution: 1.55→40.06 Å
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