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- PDB-4c94: Crystal Structure of the Strawberry Pathogenesis-Related 10 (PR-1... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4c94 | ||||||
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Title | Crystal Structure of the Strawberry Pathogenesis-Related 10 (PR-10) Fra a 3 protein in complex with Catechin | ||||||
![]() | FRA A 3 ALLERGEN | ||||||
![]() | ALLERGEN / PYR/PYL/RCAR / BET V 1 / FLAVONOIDS | ||||||
Function / homology | ![]() flavonoid biosynthetic process / response to biotic stimulus / abscisic acid binding / abscisic acid-activated signaling pathway / protein phosphatase inhibitor activity / defense response / signaling receptor activity Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Casanal, A. / Zander, U. / Valpuesta, V. / Marquez, J.A. | ||||||
![]() | ![]() Title: The Strawberry Pathogenesis-Related 10 (Pr-10) Fra a Proteins Control Flavonoid Biosynthesis by Binding to Metabolic Intermediates. Authors: Casanal, A. / Zander, U. / Munoz, C. / Dupeux, F. / Luque, I. / Botella, M.A. / Schwab, W. / Valpuesta, V. / Marquez, J.A. #1: Journal: Acta Crystallogr.,Sect.F / Year: 2013 Title: Purification, Crystallization and Preliminary X-Ray Analysis of the Strawberry Allergens Fra a 1E and Fra a 3 in the Presence of Catechin. Authors: Casanal, A. / Zander, U. / Dupeux, F. / Valpuesta, V. / Marquez, J.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 165 KB | Display | ![]() |
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PDB format | ![]() | 134.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.2 MB | Display | ![]() |
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Full document | ![]() | 2.3 MB | Display | |
Data in XML | ![]() | 34.8 KB | Display | |
Data in CIF | ![]() | 44.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4c9cSC ![]() 4c9iC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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5 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 17616.994 Da / Num. of mol.: 5 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-KXN / ( #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 7.09 Å3/Da / Density % sol: 82.66 % / Description: NONE |
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Crystal grow | pH: 7 / Details: 2.25 M SODIUM MALONATE, PH 7.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Nov 7, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9334 Å / Relative weight: 1 |
Reflection | Resolution: 3→30 Å / Num. obs: 49904 / % possible obs: 99.5 % / Observed criterion σ(I): 2 / Redundancy: 7.4 % / Rmerge(I) obs: 0.03 / Net I/σ(I): 21.7 |
Reflection shell | Resolution: 3→30 Å |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 4C9C Resolution: 3→29.67 Å / Cor.coef. Fo:Fc: 0.955 / Cor.coef. Fo:Fc free: 0.941 / SU B: 9.271 / SU ML: 0.167 / Cross valid method: THROUGHOUT / ESU R: 0.291 / ESU R Free: 0.223 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 74.664 Å2
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Refinement step | Cycle: LAST / Resolution: 3→29.67 Å
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Refine LS restraints |
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