PDB-4by6: Yeast Not1-Not2-Not5 complex

ID or keywords:

Entry

Database: PDB / ID: 4by6

Title

Yeast Not1-Not2-Not5 complex

Components

(GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT ...) x 3

Keywords

TRANSCRIPTION / CTERMINAL COMPONENTS OF THE CCR4_NOT COMPLEX

Function / homology

Function and homology information

response to pheromone triggering conjugation with cellular fusion / negative regulation of cytoplasmic mRNA processing body assembly / pseudohyphal growth / deadenylation-independent decapping of nuclear-transcribed mRNA / CCR4-NOT core complex / nuclear-transcribed mRNA poly(A) tail shortening / deadenylation-dependent decapping of nuclear-transcribed mRNA / nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / ATPase activator activity /

P-body ...
Similarity search - Function

Biological species

SACCHAROMYCES CEREVISIAE (brewer's yeast)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

SAD / Resolution: 2.797 Å

Authors

Bhaskar, V. / Basquin, J. / Conti, E.

Citation

Journal: Nat.Struct.Mol.Biol. / Year: 2013
Title: Structure and RNA-Binding Properties of the not1-not2-not5 Module of the Yeast Ccr4-not Complex
Authors: Bhaskar, V. / Roudko, V. / Basquin, J. / Sharma, K. / Urlaub, H. / Seraphin, B. / Conti, E.

History

Deposition	Jul 18, 2013	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Oct 16, 2013	Provider: repository / Type: Initial release
Revision 1.1	Oct 23, 2013	Group: Database references
Revision 1.2	Nov 20, 2013	Group: Database references

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	4by6.cif.gz	717 KB	Display	PDBx/mmCIF format
PDB format	pdb4by6.ent.gz	608.3 KB	Display	PDB format
PDBx/mmJSON format	4by6.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/by/4by6 ftp://data.pdbj.org/pub/pdb/validation_reports/by/4by6	HTTPS FTP

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Related structure data

Similar structure data	3cke-2 1jq3-d 5e2j-d 6b1b-d 5l8s-2 6a98-d 1ytu-d 3qw4-3 Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI

Deposited unit

A: GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 1

B: GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 2

C: GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 5

D: GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 1

E: GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 2

F: GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 5

hetero molecules

237 kDa, 6 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

D: GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 1

E: GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 2

F: GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 5

hetero molecules

defined by software
118 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

A: GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 1

B: GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 2

C: GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 5

hetero molecules

defined by software
118 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	110.452, 109.174, 133.622
Angle α, β, γ (deg.)	90.00, 94.70, 90.00
Int Tables number	4
Space group name H-M	P12₁1

Noncrystallographic symmetry (NCS)

NCS domain:

ID	Ens-ID
1	1
2	2
3	3

/ NCS ensembles :

ID
1
2
3

NCS oper: (Code: given
Matrix: (-0.0994, 0.0027, -0.995), (0.0077, -1, -0.0035), (-0.995, -0.008, 0.0993)
Vector: 250.6354, 5.7417, 205.6984)

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GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT ... , 3 types, 6 molecules ADBECF

#1: Protein	GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 1 / CELL DIVISION CYCLE PROTEIN 39 / NOT1 CTERM Mass: 64436.094 Da / Num. of mol.: 2 / Fragment: RESIDUES 1542-2094 Source method: isolated from a genetically manipulated source Source: (gene. exp.) SACCHAROMYCES CEREVISIAE (brewer's yeast) Strain: CEN.PK113-7D / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21 / Variant (production host): GOLD / References: UniProt: N1P976, UniProt: P25655*PLUS
#2: Protein	GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 2 / CELL DIVISION CYCLE PROTEIN 36 / NOT2 Mass: 22387.469 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) SACCHAROMYCES CEREVISIAE (brewer's yeast) Strain: S288C / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21 / Variant (production host): GOLD / References: UniProt: P06100
#3: Protein	GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 5 / NOT5 Mass: 31208.912 Da / Num. of mol.: 2 / Fragment: RESIDUES 299-560 Source method: isolated from a genetically manipulated source Source: (gene. exp.) SACCHAROMYCES CEREVISIAE (brewer's yeast) Strain: S288C / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21 / Variant (production host): GOLD / References: UniProt: Q12514

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Non-polymers , 5 types, 13 molecules

#4: Chemical	ChemComp-CA / CALCIUM ION Mass: 40.078 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Ca
#5: Chemical	ChemComp-ACT / ACETATE ION / Acetate Mass: 59.044 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C₂H₃O₂
#6: Chemical	ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL / Glycerol Mass: 92.094 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C₃H₈O₃
#7: Chemical	ChemComp-PEG / DI(HYDROXYETHYL)ETHER / Diethylene glycol Mass: 106.120 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C₄H₁₀O₃
#8: Water	ChemComp-HOH / water / Water Mass: 18.015 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 3.38 Å³/Da / Density % sol: 63 % / Description: NONE
Crystal grow	pH: 6.5 Details: 6.5% (W/V) PEG 8000, 100 MM MES 6.5, 200 MM CALCIUM ACETATE

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Data collection

Diffraction

ID	Mean temperature (K)	Crystal-ID
1	100	1
2		1

Diffraction source

Source	Site	Beamline	ID	Wavelength
SYNCHROTRON	SLS	X06DA	1	1
SYNCHROTRON	SLS	X10SA	2	1.00606

Detector

Type	ID	Detector	Date
DECTRIS PILATUS	1	PIXEL	Oct 16, 2012
DECTRIS PILATUS	2	PIXEL

Radiation

ID	Protocol	Monochromatic (M) / Laue (L)	Scattering type	Wavelength-ID
1	SINGLE WAVELENGTH	M	x-ray	1
2		M	x-ray	1

Radiation wavelength

ID	Wavelength (Å)	Relative weight
1	1	1
2	1.00606	1

Reflection

Resolution: 2.8→49.15 Å / Num. obs: 77882 / % possible obs: 97 % / Observed criterion σ(I): 2.9 / Redundancy: 4.8 % / B_iso Wilson estimate: 53.09 Å² / R_merge(I) obs: 0.06 / Net I/σ(I): 17.3

Reflection shell

Resolution: 2.8→2.95 Å / Redundancy: 4.5 % / R_merge(I) obs: 0.42 / Mean I/σ(I) obs: 2.9 / % possible all: 97

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Processing

Software

Name	Version	Classification
PHENIX	(PHENIX.REFINE)	refinement
XDS		data reduction
XSCALE		data scaling
PHENIX	AUTOSOL	phasing

Refinement

Method to determine structure:

SAD
Starting model: NONE

Resolution: 2.797→48.904 Å / SU ML: 0.37 / σ(F): 1.18 / Phase error: 23.03 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection
R_free	0.2258	7524	5 %
R_work	0.1812	-	-
obs	0.1834	77872	98.04 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 67 Å²

Refinement step

Cycle: LAST / Resolution: 2.797→48.904 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	13987	0	36	5	14028

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.01	14382
X-RAY DIFFRACTION	f_angle_d	1.117	19505
X-RAY DIFFRACTION	f_dihedral_angle_d	14.044	5238
X-RAY DIFFRACTION	f_chiral_restr	0.044	2160
X-RAY DIFFRACTION	f_plane_restr	0.006	2451

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.7971-2.8289	0.3142	206	0.3083	4016	X-RAY DIFFRACTION	81
2.8289-2.8622	0.3591	249	0.2872	4714	X-RAY DIFFRACTION	99
2.8622-2.8971	0.3511	252	0.2811	4798	X-RAY DIFFRACTION	99
2.8971-2.9337	0.3404	252	0.2763	4840	X-RAY DIFFRACTION	99
2.9337-2.9723	0.3332	259	0.2625	4853	X-RAY DIFFRACTION	99
2.9723-3.013	0.3082	252	0.2533	4838	X-RAY DIFFRACTION	99
3.013-3.0561	0.2939	250	0.2378	4791	X-RAY DIFFRACTION	99
3.0561-3.1017	0.3096	256	0.2278	4862	X-RAY DIFFRACTION	99
3.1017-3.1501	0.31	250	0.2277	4820	X-RAY DIFFRACTION	99
3.1501-3.2018	0.245	254	0.2132	4854	X-RAY DIFFRACTION	99
3.2018-3.257	0.2743	257	0.219	4817	X-RAY DIFFRACTION	99
3.257-3.3162	0.2536	255	0.2218	4792	X-RAY DIFFRACTION	99
3.3162-3.38	0.2529	255	0.214	4802	X-RAY DIFFRACTION	99
3.38-3.4489	0.2629	254	0.1966	4803	X-RAY DIFFRACTION	98
3.4489-3.5239	0.2603	254	0.1969	4821	X-RAY DIFFRACTION	99
3.5239-3.6059	0.2437	254	0.1845	4747	X-RAY DIFFRACTION	98
3.6059-3.696	0.2004	249	0.1664	4724	X-RAY DIFFRACTION	97
3.696-3.7959	0.2363	250	0.1675	4787	X-RAY DIFFRACTION	99
3.7959-3.9075	0.2031	249	0.1683	4784	X-RAY DIFFRACTION	98
3.9075-4.0336	0.2118	250	0.1577	4761	X-RAY DIFFRACTION	98
4.0336-4.1777	0.2005	249	0.1568	4808	X-RAY DIFFRACTION	98
4.1777-4.3449	0.1761	250	0.1403	4725	X-RAY DIFFRACTION	98
4.3449-4.5425	0.1669	254	0.1312	4835	X-RAY DIFFRACTION	99
4.5425-4.7818	0.1721	254	0.1279	4844	X-RAY DIFFRACTION	99
4.7818-5.0811	0.1832	251	0.1342	4790	X-RAY DIFFRACTION	99
5.0811-5.4729	0.1948	255	0.1508	4815	X-RAY DIFFRACTION	99
5.4729-6.0228	0.2215	254	0.1699	4808	X-RAY DIFFRACTION	98
6.0228-6.8922	0.1998	254	0.1716	4784	X-RAY DIFFRACTION	98
6.8922-8.6756	0.1742	247	0.1624	4708	X-RAY DIFFRACTION	97
8.6756-48.9118	0.2164	249	0.1923	4782	X-RAY DIFFRACTION	98

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	6.9141	-1.9592	-0.5366	5.4401	0.6172	4.9485	-0.0091	-0.4159	0.1902	0.1136	-0.034	-0.0884	-0.1181	0.0789	0.0535	0.278	-0.0477	-0.0548	0.3166	0.0199	0.2157	15.2484	-8.6881	213.9236
2	2.3713	0.4887	0.3604	1.581	0.2615	1.7058	-0.0781	-0.0512	-0.1355	0.0462	0.0151	-0.0027	0.1	-0.2186	0.0822	0.276	-0.0712	-0.0043	0.3251	0.032	0.3241	-1.3308	-11.1136	204.0627
3	2.4384	1.2864	3.0665	1.9404	1.2986	3.9415	-0.1904	-0.1126	0.2501	-0.1161	-0.4783	0.4656	-0.2471	-0.6395	0.58	0.3678	-0.0966	0.0025	0.6018	-0.026	0.4606	-16.4184	-0.9975	196.3887
4	3.3279	0.7812	-0.4448	2.5367	-0.4846	3.2196	-0.2386	0.3697	0.1717	-0.3401	0.1364	0.1404	0.0844	-0.1805	0.0936	0.3055	-0.1047	-0.0351	0.4625	-0.01	0.3613	-32.3214	-14.8043	185.6374
5	5.0176	-1.0055	1.0507	2.7659	-3.4786	5.6407	-0.0445	0.3766	1.336	-0.7029	0.4908	0.4218	-0.8201	-1.0912	-0.3781	1.0406	0.032	-0.1852	0.6616	0.2513	1.1749	-45.196	4.2847	181.1166
6	0.3579	-0.162	-0.1773	0.1271	0.0648	0.0925	-0.0675	-0.141	0.5018	-0.4538	-0.2619	0.7026	0.1992	-0.3361	0.3166	0.5635	-0.0629	0.0876	0.5201	-0.2533	0.7879	-38.2567	2.0163	194.6806
7	5.2885	-2.8983	-0.348	5.7572	2.8268	5.2258	-0.502	0.8683	0.4832	-0.1927	0.6945	-0.772	-1.4794	0.6619	-0.1682	0.8829	-0.2937	-0.1558	0.5638	0.1255	0.8766	-12.2555	8.0624	187.631
8	6.9641	2.2213	-1.5335	1.5841	0.0791	1.5607	-0.1979	-0.0425	-0.3198	-0.034	0.0481	-0.1595	0.2245	-0.1488	0.1356	0.3344	0.0027	-0.0264	0.3196	0.1117	0.352	14.4779	3.4524	192.5169
9	5.3099	0.8128	-1.6754	7.1715	-1.5945	2.3625	-0.1489	0.0847	0.0665	-0.0989	0.2277	-0.4762	-0.3689	-0.0513	-0.0574	0.2438	0.0366	0.0024	0.3198	0.022	0.2945	25.9192	21.4193	189.7354
10	2.5722	0.1899	-0.6204	5.4639	0.4291	3.2836	-0.0678	0.1806	0.1712	-0.4068	0.1946	0.4573	-0.504	-0.2427	-0.0919	0.3464	0.0621	-0.0099	0.3848	0.0586	0.3538	15.86	35.6226	186.705
11	3.5994	0.6752	3.6624	7.3657	-0.1111	3.8218	0.2554	-1.7199	-1.0262	0.4165	-0.1731	0.631	0.7614	-1.0436	-0.0461	0.6411	-0.2073	0.1498	0.7941	0.19	0.6608	-8.3534	-16.5327	221.0063
12	7.0743	-4.0623	-2.9301	2.9014	0.6666	6.5074	-0.0954	0.4833	0.0175	0.3419	0.0587	0.2988	-0.0341	-0.916	-0.0298	0.2941	-0.0644	-0.0749	0.3416	0.0035	0.4199	-8.0356	2.4726	211.4818
13	2.7382	-0.2187	0.0335	2.151	1.6173	2.689	-0.2728	-0.6003	-0.1746	0.3848	0.3383	0.0993	-0.0819	0.0648	-0.0799	0.3834	0.0529	-0.0927	0.4683	0.1094	0.3311	21.3851	17.059	200.7053
14	5.0306	2.6013	0.1297	3.14	2.4326	3.126	0.0447	0.9569	0.1869	-1.3704	0.1526	0.1131	-0.4408	-1.2152	-0.0425	0.4731	-0.0871	-0.1428	0.6285	0.1125	0.5436	4.3397	7.3352	183.8326
15	4.1337	0.9004	-1.1026	2.8859	-0.3752	3.2666	-0.2878	0.2171	-0.4645	-0.5426	0.1024	-0.3061	0.2312	-0.0678	0.1476	0.3936	-0.0762	0.041	0.2665	-0.0073	0.3707	22.4663	2.9359	177.4394
16	6.1142	-0.0958	0.4307	6.1541	-1.4275	6.4265	0.869	0.6786	0.5919	-0.9206	-0.6241	-0.0395	-0.1626	-0.486	-0.2359	0.6749	0.2434	-0.0195	0.6794	-0.1008	0.814	15.4197	11.4552	229.2258
17	2.4474	0.0962	0.0563	1.4783	0.3407	3.289	0.2553	-0.1306	0.3924	-0.1015	-0.1104	0.3013	-0.7172	-0.3647	-0.1461	0.6451	0.125	0.1465	0.4225	-0.1043	0.6043	23.103	15.6503	246.8327
18	5.0682	0.3302	4.4521	5.8144	1.9303	4.3758	0.6297	-0.2657	-0.4712	0.6804	-0.0381	-0.0309	-0.1205	-0.0363	-0.5492	0.6485	-0.0617	0.1488	0.532	-0.1698	0.4441	35.7855	6.3864	265.3516
19	3.2241	-0.2788	-0.9026	2.2021	1.0947	4.3721	0.1012	0.0702	-0.0063	-0.2205	0.2384	-0.4404	-0.2821	0.5803	-0.3248	0.5658	-0.1641	0.1244	0.5602	-0.1446	0.4344	46	22.9208	278.5073
20	4.4645	0.4572	0.487	3.6957	0.3122	6.5353	0.2592	-0.3197	-0.6343	0.0052	0.3662	-0.5674	0.3474	0.8948	-0.5922	0.412	0.0415	-0.0067	0.623	-0.1326	0.5776	52.6756	9.5701	285.0658
21	1.9211	0.1197	1.0185	1.982	-0.8795	1.0951	0.2679	0.1376	-0.2882	0.143	0.1523	0.0371	1.3499	0.4798	-0.4082	0.708	-0.0457	0.0432	0.3482	-0.1301	0.8566	41.5776	0.2753	274.1647
22	1.7643	0.3084	-2.0538	2.5487	2.0516	5.5368	0.4437	-0.2948	0.5038	0.0897	-0.0554	0.0222	-0.792	0.1903	-0.3094	0.4814	0.0639	0.0809	0.3791	-0.1052	0.5189	38.1156	3.6468	241.2616
23	4.9969	-1.4347	-0.1184	6.9888	-0.2355	2.53	-0.1069	0.2619	-0.0957	-0.4748	0.1324	-0.0176	0.0796	0.2014	-0.0186	0.3211	0.059	0.0182	0.3642	0.0299	0.1942	37.796	-14.682	221.2582
24	2.9293	-0.5898	-0.5414	6.138	1.6772	2.842	-0.1241	-0.2831	-0.2801	0.5509	0.2828	-0.5334	0.3147	0.1465	-0.1448	0.3831	0.0712	-0.014	0.3594	0.0566	0.2777	44.67	-29.7983	233.4495
25	0.341	0.7622	-0.9461	6.501	-1.8514	2.6342	-0.0829	-0.2794	-0.2581	-0.1008	0.0816	-0.2628	0.2841	0.3816	0.011	0.4012	0.127	0.08	0.4332	0.0286	0.337	41.409	-34.0749	234.3204
26	3.953	1.1012	0.8028	0.547	-0.491	2.2848	-0.1187	-0.2415	0.1259	-0.6142	-0.7227	0.7018	-0.8482	-1.0594	0.4251	1.1429	0.345	0.3867	0.7647	-0.301	1.1878	8.12	23.0053	253.4743
27	0.0362	0.4799	-0.361	8.0761	-5.8595	4.2661	-0.3867	-1.1019	0.3154	1.4009	0.5628	0.1914	-1.0828	-1.2863	-0.1857	0.9319	0.2555	0.1799	1.1577	-0.3262	1.0161	14.2791	18.0479	269.8946
28	0.1715	0.4167	-0.1635	2.3798	1.8934	3.5429	0.2653	-0.1697	0.1664	0.1524	-0.3031	0.2804	-0.2354	-0.6099	0.0653	0.3381	0.1103	-0.0093	0.5419	-0.0061	0.5344	23.1753	-4.3398	244.3395
29	4.8363	4.6404	4.9703	4.4594	4.7615	5.1493	0.1269	-0.9434	-0.0978	0.3772	-0.016	-0.4023	-0.0299	0.378	-0.1557	0.4935	0.0907	-0.0029	0.6166	-0.0679	0.5067	43.6807	-3.8844	242.4361
30	2.933	-0.1955	-0.821	3.8934	-0.1585	3.5457	0.2595	-0.0921	0.6062	-0.2695	0.0947	-0.3791	-0.745	0.4151	-0.2928	0.4767	-0.0586	0.0902	0.3213	-0.0649	0.4366	50.5521	2.0375	224.4873

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	(CHAIN A AND RESID 1567:1657)
2	X-RAY DIFFRACTION	2	(CHAIN A AND RESID 1660:1792)
3	X-RAY DIFFRACTION	3	(CHAIN A AND RESID 1793:1825)
4	X-RAY DIFFRACTION	4	(CHAIN A AND RESID 1826:2053)
5	X-RAY DIFFRACTION	5	(CHAIN A AND RESID 2054:2079)
6	X-RAY DIFFRACTION	6	(CHAIN B AND RESID 5:32)
7	X-RAY DIFFRACTION	7	(CHAIN B AND RESID 33:49)
8	X-RAY DIFFRACTION	8	(CHAIN B AND RESID 50:99)
9	X-RAY DIFFRACTION	9	(CHAIN B AND RESID 100:138)
10	X-RAY DIFFRACTION	10	(CHAIN B AND RESID 139:191)
11	X-RAY DIFFRACTION	11	(CHAIN C AND RESID 345:373)
12	X-RAY DIFFRACTION	12	(CHAIN C AND RESID 374:402)
13	X-RAY DIFFRACTION	13	(CHAIN C AND RESID 403:423)
14	X-RAY DIFFRACTION	14	(CHAIN C AND RESID 424:467)
15	X-RAY DIFFRACTION	15	(CHAIN C AND RESID 468:560)
16	X-RAY DIFFRACTION	16	(CHAIN D AND RESID 1567:1597)
17	X-RAY DIFFRACTION	17	(CHAIN D AND RESID 1598:1792)
18	X-RAY DIFFRACTION	18	(CHAIN D AND RESID 1793:1825)
19	X-RAY DIFFRACTION	19	(CHAIN D AND RESID 1826:1992)
20	X-RAY DIFFRACTION	20	(CHAIN D AND RESID 1993:2078)
21	X-RAY DIFFRACTION	21	(CHAIN E AND RESID 4:43)
22	X-RAY DIFFRACTION	22	(CHAIN E AND RESID 48:85)
23	X-RAY DIFFRACTION	23	(CHAIN E AND RESID 86:138)
24	X-RAY DIFFRACTION	24	(CHAIN E AND RESID 139:179)
25	X-RAY DIFFRACTION	25	(CHAIN E AND RESID 180:191)
26	X-RAY DIFFRACTION	26	(CHAIN F AND RESID 345:362)
27	X-RAY DIFFRACTION	27	(CHAIN F AND RESID 363:375)
28	X-RAY DIFFRACTION	28	(CHAIN F AND RESID 376:419)
29	X-RAY DIFFRACTION	29	(CHAIN F AND RESID 420:467)
30	X-RAY DIFFRACTION	30	(CHAIN F AND RESID 468:560)

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