+Open data
-Basic information
Entry | Database: PDB / ID: 4bdv | |||||||||
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Title | CRYSTAL STRUCTURE OF A TRUNCATED B-DOMAIN HUMAN FACTOR VIII | |||||||||
Components | (FACTOR VIIIA ...) x 2 | |||||||||
Keywords | BLOOD CLOTTING / BLOOD COAGULATION / METAL BINDING | |||||||||
Function / homology | Function and homology information Defective F8 accelerates dissociation of the A2 domain / Defective F8 binding to the cell membrane / Defective F8 secretion / Gamma carboxylation, hypusinylation, hydroxylation, and arylsulfatase activation / Defective F8 sulfation at Y1699 / Defective F8 binding to von Willebrand factor / blood coagulation, intrinsic pathway / Cargo concentration in the ER / Defective factor IX causes thrombophilia / Defective cofactor function of FVIIIa variant ...Defective F8 accelerates dissociation of the A2 domain / Defective F8 binding to the cell membrane / Defective F8 secretion / Gamma carboxylation, hypusinylation, hydroxylation, and arylsulfatase activation / Defective F8 sulfation at Y1699 / Defective F8 binding to von Willebrand factor / blood coagulation, intrinsic pathway / Cargo concentration in the ER / Defective factor IX causes thrombophilia / Defective cofactor function of FVIIIa variant / Defective F9 variant does not activate FX / COPII-mediated vesicle transport / COPII-coated ER to Golgi transport vesicle / Defective F8 cleavage by thrombin / Common Pathway of Fibrin Clot Formation / Intrinsic Pathway of Fibrin Clot Formation / endoplasmic reticulum-Golgi intermediate compartment membrane / platelet alpha granule lumen / acute-phase response / Golgi lumen / blood coagulation / Platelet degranulation / signaling receptor activity / oxidoreductase activity / copper ion binding / endoplasmic reticulum lumen / extracellular space / extracellular region / plasma membrane Similarity search - Function | |||||||||
Biological species | HOMO SAPIENS (human) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 3.98 Å | |||||||||
Authors | Svensson, L.A. / Thim, L. / Olsen, O.H. / Nicolaisen, E.M. | |||||||||
Citation | Journal: Biol.Chem. / Year: 2013 Title: Evaluation of the Metal Binding Sites in a Recombinant Coagulation Factor Viii Identifies Two Sites with Unique Metal Binding Properties. Authors: Svensson, L.A. / Thim, L. / Olsen, O.H. / Nicolaisen, E.M. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4bdv.cif.gz | 533.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4bdv.ent.gz | 439.4 KB | Display | PDB format |
PDBx/mmJSON format | 4bdv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 4bdv_validation.pdf.gz | 1019.4 KB | Display | wwPDB validaton report |
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Full document | 4bdv_full_validation.pdf.gz | 1.1 MB | Display | |
Data in XML | 4bdv_validation.xml.gz | 55.4 KB | Display | |
Data in CIF | 4bdv_validation.cif.gz | 73.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bd/4bdv ftp://data.pdbj.org/pub/pdb/validation_reports/bd/4bdv | HTTPS FTP |
-Related structure data
Related structure data | 3cdzS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-FACTOR VIIIA ... , 2 types, 2 molecules AB
#1: Protein | Mass: 87107.391 Da / Num. of mol.: 1 Fragment: COAGULATION FACTOR VIII, RESIDUES 20-769,1657-1666 Source method: isolated from a genetically manipulated source Details: THE FVIII B-DOMAIN HAS BEEN REPLACED WITH A TRUNCATED, 21 AMINO ACID LONG, B-DOMAIN VARIANT. Source: (gene. exp.) HOMO SAPIENS (human) / Organ: BLOOD / Cell line (production host): CHO / Production host: CRICETULUS GRISEUS (Chinese hamster) / References: UniProt: P00451 |
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#2: Protein | Mass: 79241.797 Da / Num. of mol.: 1 / Fragment: RESIDUES 1667-2351 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Organ: BLOOD / Cell line (production host): CHO / Production host: CRICETULUS GRISEUS (Chinese hamster) / References: UniProt: P00451 |
-Sugars , 2 types, 2 molecules
#3: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
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#4: Polysaccharide | beta-D-mannopyranose-(1-3)-[beta-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2- ...beta-D-mannopyranose-(1-3)-[beta-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
-Non-polymers , 4 types, 5 molecules
#5: Chemical | ChemComp-ZN / | ||
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#6: Chemical | ChemComp-CA / | ||
#7: Chemical | #8: Chemical | ChemComp-CU1 / | |
-Details
Sequence details | THE SEQUENCE IS DESCRIBED IN THIM, L. ET AL. (2010), HAEMOPHILI |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.8 Å3/Da / Density % sol: 74 % Description: DATA USED DURING REFINEMENTS WERE A COMBINATION TWO DATA SETS COLLECTED AT TWO DIFFERENT WAVELENGTHS. |
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Crystal grow | pH: 7.5 Details: 3% PEG550, 0.100 M NACL, 0.100 M TRIS-HCL PH 7.5, 10% ETHANOL |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||
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Diffraction source | Source: SYNCHROTRON / Site: MAX II / Beamline: I911-3 / Wavelength: 1.0082, 1.2700 | |||||||||
Detector | Type: MARRESEARCH / Detector: CCD / Date: Jun 24, 2009 / Details: TWO MIRRORS | |||||||||
Radiation | Monochromator: DOUBLE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
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Reflection | Resolution: 3.98→20 Å / Num. obs: 28556 / % possible obs: 99.6 % / Observed criterion σ(I): -3 / Redundancy: 16.6 % / Rmerge(I) obs: 0.2 / Net I/σ(I): 12.45 | |||||||||
Reflection shell | Resolution: 3.98→4.08 Å / Redundancy: 17.1 % / Rmerge(I) obs: 1.4 / Mean I/σ(I) obs: 2.39 / % possible all: 99.9 |
-Processing
Software |
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Refinement | Method to determine structure: FOURIER SYNTHESIS Starting model: PDB ENTRY 3CDZ Resolution: 3.98→20 Å / Cor.coef. Fo:Fc: 0.962 / Cor.coef. Fo:Fc free: 0.909 / SU B: 79.938 / SU ML: 0.458 / Cross valid method: THROUGHOUT / ESU R Free: 0.608 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: U VALUES WITH TLS ADDED. HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 160.457 Å2
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Refinement step | Cycle: LAST / Resolution: 3.98→20 Å
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Refine LS restraints |
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