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Yorodumi- PDB-4aml: CRYSTAL STRUCTURE OF WHEAT GERM AGGLUTININ ISOLECTIN 1 IN COMPLEX... -
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-Basic information
Entry | Database: PDB / ID: 4aml | |||||||||||||||
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Title | CRYSTAL STRUCTURE OF WHEAT GERM AGGLUTININ ISOLECTIN 1 IN COMPLEX WITH GLYCOSYLURETHAN | |||||||||||||||
Components | AGGLUTININ ISOLECTIN 1 | |||||||||||||||
Keywords | SUGAR BINDING PROTEIN / SYNTHETIC CARBOHYDRATE LIGAND / PROTEIN CARBOHYDRATE INTERACTION / LECTIN / CHITIN-BINDING / CARBOHYDRATE BINDING PROTEIN | |||||||||||||||
Function / homology | Function and homology information | |||||||||||||||
Biological species | TRITICUM AESTIVUM (bread wheat) | |||||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.6 Å | |||||||||||||||
Authors | Schwefel, D. / Maierhofer, C. / Beck, J.G. / Seeberger, S. / Diederichs, K. / Moeller, H.M. / Welte, W. / Wittmann, V. | |||||||||||||||
Citation | Journal: J.Am.Chem.Soc. / Year: 2010 Title: Structural Basis of Multivalent Binding to Wheat Germ Agglutinin. Authors: Schwefel, D. / Maierhofer, C. / Beck, J.G. / Seeberger, S. / Diederichs, K. / Moller, H.M. / Welte, W. / Wittmann, V. | |||||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4aml.cif.gz | 139.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4aml.ent.gz | 111.5 KB | Display | PDB format |
PDBx/mmJSON format | 4aml.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 4aml_validation.pdf.gz | 2 MB | Display | wwPDB validaton report |
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Full document | 4aml_full_validation.pdf.gz | 2.1 MB | Display | |
Data in XML | 4aml_validation.xml.gz | 19.2 KB | Display | |
Data in CIF | 4aml_validation.cif.gz | 26.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/am/4aml ftp://data.pdbj.org/pub/pdb/validation_reports/am/4aml | HTTPS FTP |
-Related structure data
Related structure data | 2uvoC 2x3tC 2x52C 7wgaS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 17124.080 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) TRITICUM AESTIVUM (bread wheat) / References: UniProt: P10968 #2: Sugar | ChemComp-GYU / #3: Chemical | #4: Water | ChemComp-HOH / | Sequence details | THE PRECURSOR IS PROCESSED TO THE MATURE PROTEIN COMPRISING AMINO ACID RESIDUES 27 TO 197. RESIDUE ...THE PRECURSOR IS PROCESSED TO THE MATURE PROTEIN COMPRISING | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.59 Å3/Da / Density % sol: 52.44 % / Description: NONE |
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Crystal grow | Details: 0.04 M POTASSIUM PHOSPHATE 14 % PEG 8000 20 % GLYCEROL |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 1 |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Oct 27, 2006 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→10 Å / Num. obs: 46032 / % possible obs: 96.1 % / Observed criterion σ(I): 2 / Redundancy: 9.53 % / Rmerge(I) obs: 0.06 / Net I/σ(I): 17.38 |
Reflection shell | Resolution: 1.6→1.65 Å / Redundancy: 8.3 % / Rmerge(I) obs: 0.47 / Mean I/σ(I) obs: 3.52 / % possible all: 89.8 |
-Processing
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 7WGA Resolution: 1.6→54.78 Å / Cor.coef. Fo:Fc: 0.962 / Cor.coef. Fo:Fc free: 0.948 / SU B: 3.836 / SU ML: 0.072 / Cross valid method: THROUGHOUT / ESU R: 0.093 / ESU R Free: 0.095 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 33.462 Å2
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Refinement step | Cycle: LAST / Resolution: 1.6→54.78 Å
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Refine LS restraints |
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