+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 4am6 | ||||||
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タイトル | C-TERMINAL DOMAIN OF ACTIN-RELATED PROTEIN ARP8 FROM S. CEREVISIAE | ||||||
要素 | ACTIN-LIKE PROTEIN ARP8 | ||||||
キーワード | NUCLEAR PROTEIN / CHROMATIN REMODELLING COMPLEX / ATP-BINDING PROTEIN / NUCLEAR ACTIN-RELATED PROTEIN / TRANSCRIPTION REGULATION / DNA REPAIR | ||||||
機能・相同性 | 機能・相同性情報 Ino80 complex / mitotic recombination / double-strand break repair / cytoskeleton / chromatin remodeling / DNA repair / mRNA binding / DNA damage response / regulation of DNA-templated transcription / ATP binding ...Ino80 complex / mitotic recombination / double-strand break repair / cytoskeleton / chromatin remodeling / DNA repair / mRNA binding / DNA damage response / regulation of DNA-templated transcription / ATP binding / nucleus / cytoplasm 類似検索 - 分子機能 | ||||||
生物種 | SACCHAROMYCES CEREVISIAE (パン酵母) | ||||||
手法 | X線回折 / シンクロトロン / 多波長異常分散 / 解像度: 2.7 Å | ||||||
データ登録者 | Wuerges, J. / Saravanan, M. / Bose, D. / Cook, N.J. / Zhang, X. / Wigley, D.B. | ||||||
引用 | ジャーナル: Proc Natl Acad Sci U S A / 年: 2012 タイトル: Interactions between the nucleosome histone core and Arp8 in the INO80 chromatin remodeling complex. 著者: Matheshwaran Saravanan / Jochen Wuerges / Daniel Bose / Elizabeth A McCormack / Nicola J Cook / Xiaodong Zhang / Dale B Wigley / 要旨: Actin-related protein Arp8 is a component of the INO80 chromatin remodeling complex. Yeast Arp8 (yArp8) comprises two domains: a 25-KDa N-terminal domain, found only in yeast, and a 75-KDa C-terminal ...Actin-related protein Arp8 is a component of the INO80 chromatin remodeling complex. Yeast Arp8 (yArp8) comprises two domains: a 25-KDa N-terminal domain, found only in yeast, and a 75-KDa C-terminal domain (yArp8CTD) that contains the actin fold and is conserved across other species. The crystal structure shows that yArp8CTD contains three insertions within the actin core. Using a combination of biochemistry and EM, we show that Arp8 forms a complex with nucleosomes, and that the principal interactions are via the H3 and H4 histones, mediated through one of the yArp8 insertions. We show that recombinant yArp8 exists in monomeric and dimeric states, but the dimer is the biologically relevant form required for stable interactions with histones that exploits the twofold symmetry of the nucleosome core. Taken together, these data provide unique insight into the stoichiometry, architecture, and molecular interactions between components of the INO80 remodeling complex and nucleosomes, providing a first step toward building up the structure of the complex. | ||||||
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 4am6.cif.gz | 519.4 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb4am6.ent.gz | 434.8 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 4am6.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 4am6_validation.pdf.gz | 466.2 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 4am6_full_validation.pdf.gz | 522 KB | 表示 | |
XML形式データ | 4am6_validation.xml.gz | 48.8 KB | 表示 | |
CIF形式データ | 4am6_validation.cif.gz | 65.5 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/am/4am6 ftp://data.pdbj.org/pub/pdb/validation_reports/am/4am6 | HTTPS FTP |
-関連構造データ
-リンク
-集合体
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非結晶学的対称性 (NCS) | NCSドメイン:
NCSドメイン領域: Ens-ID: 1
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