Journal: J.Biol.Chem. / Year: 2012 Title: Metabolism of Vertebrate Amino Sugars with N-Glycolyl Groups: Intracellular Beta-O-Linked N-Glycolylglucosamine (Glcngc), Udp-Glcngc, and the Biochemical and Structural Rationale for the ...Title: Metabolism of Vertebrate Amino Sugars with N-Glycolyl Groups: Intracellular Beta-O-Linked N-Glycolylglucosamine (Glcngc), Udp-Glcngc, and the Biochemical and Structural Rationale for the Substrate Tolerance of Beta-O-Linked Beta-N-Acetylglucosaminidase. Authors: Macauley, M.S. / Chan, J. / Zandberg, W.F. / He, Y. / Whitworth, G.E. / Stubbs, K.A. / Yuzwa, S.A. / Bennet, A.J. / Varki, A. / Davies, G.J. / Vocadlo, D.J.
Resolution: 2.25→45.71 Å / Cor.coef. Fo:Fc: 0.954 / Cor.coef. Fo:Fc free: 0.922 / SU B: 6.33 / SU ML: 0.154 / Cross valid method: THROUGHOUT / ESU R: 0.237 / ESU R Free: 0.206 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT. U VALUES REFINED INDIVIDUALLY
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.24672
1968
5.1 %
RANDOM
Rwork
0.19538
-
-
-
obs
0.19804
36481
99.18 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK