cellulose binding / cellulase / cellulase activity / cellulose catabolic process / extracellular region / metal ion binding Similarity search - Function
Coagulation Factor XIII; Chain A, domain 1 - #70 / Auxiliary Activity family 9 / Auxiliary Activity family 9 (formerly GH61) / Coagulation Factor XIII; Chain A, domain 1 / Distorted Sandwich / Mainly Beta Similarity search - Domain/homology
Mass: 24418.043 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: METHYLATION AT 4-N-H1, AND TWO CYSTINES AT C56-C178 AND C97-C101. N-ACETYLGLUCOSAMINE GLYCOSYLATION AT N138. Source: (gene. exp.) THERMOASCUS AURANTIACUS (fungus) / Production host: ASPERGILLUS ORYZAE (mold) / Strain (production host): JAL250 / References: UniProt: G3XAP7*PLUS, cellulase
Mass: 18.015 Da / Num. of mol.: 196 / Source method: isolated from a natural source / Formula: H2O
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Details
Sequence details
SIGNAL SEQUENCE CLEAVED GENE PRODUCT STARTING AT HGFVQ... GENBANK ID ABW56451.1
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.23 Å3/Da / Density % sol: 44.82 % / Description: NONE
Crystal grow
pH: 7.5 Details: 25% PEG3350, 0.1M BISTRIS PH 5.5, 0.2 M AMMONIUM ACETATE AS RESERVOIR. AFTERWARDS THE CRYSTAL WAS SOAKED IN A SOLUTION CONSISTING OF RESERVOIR TO WHICH CU(NO3)2 HAD BEEN ADDED TO A FINAL CONCENTRATION OF 10 MM
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Data collection
Diffraction
Mean temperature: 100 K
Diffraction source
Source: SYNCHROTRON / Site: MAX II / Beamline: I911-2 / Wavelength: 1.038
Detector
Type: MARRESEARCH SX-165 / Detector: CCD / Date: Mar 17, 2011 / Details: MULTILAYER MIRROR, CURVED TO FOCUS IN THE VERTICAL
Radiation
Monochromator: BENT SI (111) CRYSTAL, HORIZONTALLY FOCUSING / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 1.038 Å / Relative weight: 1
Reflection
Resolution: 1.25→30 Å / Num. obs: 106535 / % possible obs: 93.2 % / Observed criterion σ(I): 3 / Redundancy: 2.5 % / Rmerge(I) obs: 0.04 / Net I/σ(I): 22.3
Reflection shell
Resolution: 1.25→1.28 Å / Redundancy: 1.82 % / Rmerge(I) obs: 0.14 / Mean I/σ(I) obs: 6 / % possible all: 83.2
Resolution: 1.25→27.81 Å / Cor.coef. Fo:Fc: 0.978 / Cor.coef. Fo:Fc free: 0.973 / SU B: 1.326 / SU ML: 0.027 / Cross valid method: THROUGHOUT / ESU R: 0.048 / ESU R Free: 0.045 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. THE RESIDUES 26 AND 27 ARE UNORDERED AS SEEN IN PDB ID 2YET.
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.17925
2793
5 %
RANDOM
Rwork
0.15511
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obs
0.1563
53066
100 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK