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Open data
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Basic information
Entry | Database: PDB / ID: 2gzs | ||||||
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Title | Enterobactin Hydolase IroE Complex with DFP | ||||||
![]() | IroE protein | ||||||
![]() | HYDROLASE / Enterobactin / Salmochelin / DFP / Catalytic Dyad | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Larsen, N.A. / Walsh, C.T. | ||||||
![]() | ![]() Title: Structural Characterization of Enterobactin Hydrolase IroE. Authors: Larsen, N.A. / Lin, H. / Wei, R. / Fischbach, M.A. / Walsh, C.T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 64.3 KB | Display | ![]() |
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PDB format | ![]() | 49.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 439.9 KB | Display | ![]() |
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Full document | ![]() | 446.9 KB | Display | |
Data in XML | ![]() | 14.3 KB | Display | |
Data in CIF | ![]() | 20.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 31014.004 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-DFP / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.81 Å3/Da / Density % sol: 31.92 % |
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Crystal grow | Temperature: 296 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 5-10% PEG 3350, 100 mM HEPES, 500 mM DFP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
-Data collection
Diffraction | Mean temperature: 120 K | ||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Feb 10, 2006 | ||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 1.4→20 Å / Num. all: 80830 / Num. obs: 79213 / % possible obs: 93.7 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 | ||||||||||||
Reflection shell | Resolution: 1.4→1.45 Å / % possible all: 84.6 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refine analyze | Num. disordered residues: 0 / Occupancy sum hydrogen: 0 / Occupancy sum non hydrogen: 2162 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.4→20 Å
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Refine LS restraints |
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