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Yorodumi- PDB-3znl: H5 Haemagglutinin in Complex with 6-O-Sulfo-Sialyl-Lewis X (Sulfa... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3znl | |||||||||
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Title | H5 Haemagglutinin in Complex with 6-O-Sulfo-Sialyl-Lewis X (Sulfated Lewis X) | |||||||||
Components | (HAEMAGGLUTININHemagglutinin) x 2 | |||||||||
Keywords | VIRAL PROTEIN / SIALIC ACID / GLYCOPROTEIN / VIRUS RECEPTOR / SULFATED SIALOSIDE / FUCOSYLATED SIALOSIDE / SULFATION / FUCOSYLATION / AVIAN FLU / SIALYLLACTOSAMINE | |||||||||
Function / homology | Function and homology information clathrin-dependent endocytosis of virus by host cell / host cell surface receptor binding / apical plasma membrane / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane Similarity search - Function | |||||||||
Biological species | INFLUENZA A VIRUS | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5 Å | |||||||||
Authors | Xiong, X. / Tuzikov, A. / Coombs, P. / Martin, S.R. / Walker, P.A. / Gamblin, S.J. / Bovin, N. / Skehel, J.J. | |||||||||
Citation | Journal: Virus Res. / Year: 2013 Title: Recognition of Sulphated and Fucosylated Receptor Sialosides by A/Vietnam/1194/2004 (H5N1) Influenza Virus. Authors: Xiong, X. / Tuzikov, A. / Coombs, P. / Martin, S. / Walker, P.A. / Gamblin, S.J. / Bovin, N. / Skehel, J.J. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3znl.cif.gz | 599 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3znl.ent.gz | 500.8 KB | Display | PDB format |
PDBx/mmJSON format | 3znl.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zn/3znl ftp://data.pdbj.org/pub/pdb/validation_reports/zn/3znl | HTTPS FTP |
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-Related structure data
Related structure data | 3znkC 3znmC 2ibxS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein , 2 types, 6 molecules ACEBDF
#1: Protein | Mass: 36950.766 Da / Num. of mol.: 3 Fragment: HA1 OF TRYPSIN RELEASED ECTODOMAIN, RESIDUES 17-340 Source method: isolated from a natural source Details: THE NATIONAL INSTITUTE FOR BIOLOGICAL STANDARDS AND CONTROL (NIBSC) Source: (natural) INFLUENZA A VIRUS / Variant: A/VN/1194/04/NIBRG14 VACCINE STRAIN / Strain: A/VIETNAM/1194/2004 (H5N1) / References: UniProt: Q6DQ34 #2: Protein | Mass: 19097.990 Da / Num. of mol.: 3 Fragment: HA2 OF TRYPSIN RELEASED ECTODOMAIN, RESIDUES 347-512 Source method: isolated from a natural source Details: THE NATIONAL INSTITUTE FOR BIOLOGICAL STANDARDS AND CONTROL (NIBSC) Source: (natural) INFLUENZA A VIRUS / Variant: A/VN/1194/04/NIBRG14 VACCINE STRAIN / Strain: A/VIETNAM/1194/2004 (H5N1) / References: UniProt: Q6DQ34 |
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-Sugars , 2 types, 12 molecules
#3: Polysaccharide | Source method: isolated from a genetically manipulated source #4: Sugar | ChemComp-NAG / |
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-Non-polymers , 2 types, 268 molecules
#5: Chemical | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.04 Å3/Da / Density % sol: 70 % Description: DATA CORRECTED FOR ANISOTROPY USING UCLA MBI - DIFFRACTION ANISOTROPY SERVER RETAINING 3 SIGMA DATA. |
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Crystal grow | Details: 0.1 M HEPES PH 7.0, 0.05 M MGCL2, 28 - 30 % PEG 550 MME |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04-1 / Wavelength: 0.9173 |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Apr 23, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9173 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→150.76 Å / Num. obs: 64504 / % possible obs: 70.4 % / Observed criterion σ(I): 3 / Redundancy: 3.3 % / Biso Wilson estimate: 52.4 Å2 / Rmerge(I) obs: 0.11 / Net I/σ(I): 7.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2IBX Resolution: 2.5→35.7 Å / Cor.coef. Fo:Fc: 0.892 / Cor.coef. Fo:Fc free: 0.869 / SU B: 20.107 / SU ML: 0.23 / Cross valid method: THROUGHOUT / ESU R: 0.672 / ESU R Free: 0.327 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. COMPLETENESS VS. RESOLUTION TABLE 999.99 5.39 9323 8903 5.39 4.28 9221 9089 4.28 3.74 9137 9040 3.74 3.40 9119 8419 3.40 3.15 9195 8800 3. ...Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. COMPLETENESS VS. RESOLUTION TABLE 999.99 5.39 9323 8903 5.39 4.28 9221 9089 4.28 3.74 9137 9040 3.74 3.40 9119 8419 3.40 3.15 9195 8800 3.15 2.97 9103 8814 2.97 2.82 9055 6258 2.82 2.70 9172 3148 2.70 2.59 9067 1560 2.59 2.50 9156 473
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 45.063 Å2
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Refinement step | Cycle: LAST / Resolution: 2.5→35.7 Å
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Refine LS restraints |
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