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Open data
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Basic information
Entry | Database: PDB / ID: 3wu1 | ||||||
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Title | Crystal structure of the ETS1-RUNX1-DNA ternary complex | ||||||
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![]() | TRANSCRIPTION/DNA / PROTEIN-DNA COMPLEX / DNA-BINDING / METHYLATION / NUCLEUS / PHOSPHOPROTEIN / TRANSCRIPTION REGULATION / ISOPEPTIDE BOND / PROTO-ONCOGENE / TRANSCRIPTION-DNA COMPLEX | ||||||
Function / homology | ![]() regulation of hair follicle cell proliferation / Organic cation transport / positive regulation of progesterone secretion / RUNX1 regulates estrogen receptor mediated transcription / RUNX1 regulates transcription of genes involved in BCR signaling / RUNX1 regulates transcription of genes involved in interleukin signaling / Regulation of RUNX1 Expression and Activity / RUNX1 and FOXP3 control the development of regulatory T lymphocytes (Tregs) / RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known / positive regulation of granulocyte differentiation ...regulation of hair follicle cell proliferation / Organic cation transport / positive regulation of progesterone secretion / RUNX1 regulates estrogen receptor mediated transcription / RUNX1 regulates transcription of genes involved in BCR signaling / RUNX1 regulates transcription of genes involved in interleukin signaling / Regulation of RUNX1 Expression and Activity / RUNX1 and FOXP3 control the development of regulatory T lymphocytes (Tregs) / RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known / positive regulation of granulocyte differentiation / core-binding factor complex / RUNX1 regulates transcription of genes involved in differentiation of myeloid cells / positive regulation of CD8-positive, alpha-beta T cell differentiation / positive regulation of cell maturation / negative regulation of CD4-positive, alpha-beta T cell differentiation / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / RUNX1 regulates transcription of genes involved in differentiation of HSCs / PML body organization / neuron fate commitment / Estrogen-dependent gene expression / myeloid progenitor cell differentiation / definitive hemopoiesis / regulation of T cell anergy / embryonic hemopoiesis / positive regulation of leukocyte adhesion to vascular endothelial cell / hair follicle morphogenesis / behavioral response to pain / regulation of cell differentiation / hemopoiesis / negative regulation of cell cycle / basement membrane / regulation of signal transduction / neuron development / positive regulation of blood vessel endothelial cell migration / regulation of angiogenesis / chondrocyte differentiation / response to retinoic acid / cellular response to transforming growth factor beta stimulus / positive regulation of interleukin-2 production / positive regulation of endothelial cell migration / ossification / transcription corepressor binding / positive regulation of erythrocyte differentiation / nuclear receptor coactivator activity / liver development / skeletal system development / central nervous system development / cell motility / promoter-specific chromatin binding / neuron differentiation / Oncogene Induced Senescence / positive regulation of miRNA transcription / positive regulation of inflammatory response / positive regulation of angiogenesis / sequence-specific double-stranded DNA binding / positive regulation of type II interferon production / DNA-binding transcription activator activity, RNA polymerase II-specific / regulation of apoptotic process / DNA-binding transcription factor binding / in utero embryonic development / RNA polymerase II-specific DNA-binding transcription factor binding / transcription by RNA polymerase II / nucleic acid binding / transcription cis-regulatory region binding / DNA-binding transcription factor activity, RNA polymerase II-specific / immune response / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of cell population proliferation / response to antibiotic / negative regulation of DNA-templated transcription / positive regulation of gene expression / regulation of DNA-templated transcription / chromatin / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / protein-containing complex / DNA binding / nucleoplasm / ATP binding / identical protein binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Tahirov, T.H. / Ogata, K. | ||||||
![]() | ![]() Title: A novel allosteric mechanism on protein-DNA interactions underlying the phosphorylation-dependent regulation of Ets1 target gene expressions. Authors: Shiina, M. / Hamada, K. / Inoue-Bungo, T. / Shimamura, M. / Uchiyama, A. / Baba, S. / Sato, K. / Yamamoto, M. / Ogata, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 83 KB | Display | ![]() |
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PDB format | ![]() | 58 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 447.8 KB | Display | ![]() |
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Full document | ![]() | 454.6 KB | Display | |
Data in XML | ![]() | 13.7 KB | Display | |
Data in CIF | ![]() | 19 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3wtsC ![]() 3wttC ![]() 3wtuC ![]() 3wtvC ![]() 3wtwC ![]() 3wtxC ![]() 3wtyC ![]() 3wtzC ![]() 3wu0C ![]() 1gvjS ![]() 1io4S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 13540.438 Da / Num. of mol.: 1 / Fragment: UNP RESIDUES 55-177 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Protein | Mass: 12970.817 Da / Num. of mol.: 1 / Fragment: UNP RESIDUES 333-441 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
#3: DNA chain | Mass: 4843.156 Da / Num. of mol.: 1 / Source method: obtained synthetically |
#4: DNA chain | Mass: 4954.214 Da / Num. of mol.: 1 / Source method: obtained synthetically |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.75 Å3/Da / Density % sol: 74.11 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion / pH: 7.5 Details: 11% PEG MME 550, 0.1M KCL, 0.015M MGCL2, 0.05M TRIS, pH 7.5, VAPOR DIFFUSION, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RIGAKU JUPITER 210 / Detector: CCD / Date: May 25, 2001 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.02 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→30 Å / Num. obs: 23925 / % possible obs: 87.4 % / Redundancy: 4.9 % / Rmerge(I) obs: 0.049 / Net I/σ(I): 13.1 |
Reflection shell | Resolution: 2.4→2.44 Å / Redundancy: 2.7 % / Rmerge(I) obs: 0.352 / % possible all: 65.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1IO4, 1GVJ Resolution: 2.4→29.78 Å / σ(F): 0
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Solvent computation | Bsol: 38.09 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 46.23 Å2
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Refinement step | Cycle: LAST / Resolution: 2.4→29.78 Å
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Xplor file |
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