- PDB-3ws1: N288Q-N321Q mutant BETA-LACTAMASE DERIVED FROM CHROMOHALOBACTER S... -
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基本情報
登録情報
データベース: PDB / ID: 3ws1
タイトル
N288Q-N321Q mutant BETA-LACTAMASE DERIVED FROM CHROMOHALOBACTER SP.560 (Condition-1B)
要素
Beta-lactamase
キーワード
HYDROLASE / CEPHALOSPORINASE
機能・相同性
機能・相同性情報
antibiotic catabolic process / beta-lactamase activity / beta-lactamase / outer membrane-bounded periplasmic space / response to antibiotic / metal ion binding 類似検索 - 分子機能
温度: 293 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 7 詳細: Crystal was grown in a solution containing 100mM MES-NaOH buffer (pH 6.5), 200mM Ca acetate hydrate and 18% PEG8000. Obtained crystal was soaked into a solution containing 100mM MES NaOH ...詳細: Crystal was grown in a solution containing 100mM MES-NaOH buffer (pH 6.5), 200mM Ca acetate hydrate and 18% PEG8000. Obtained crystal was soaked into a solution containing 100mM MES NaOH buffer (pH 6.5), 200mM Ca acetate hydrate, 75mM NaCl, 25mM CsCl and 18% PEG8000., VAPOR DIFFUSION, SITTING DROP, temperature 293K
解像度: 1.8→37.68 Å / Cor.coef. Fo:Fc: 0.954 / Cor.coef. Fo:Fc free: 0.943 / SU B: 5.356 / SU ML: 0.09 / 交差検証法: THROUGHOUT / ESU R: 0.139 / ESU R Free: 0.123 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.21118
4618
5 %
RANDOM
Rwork
0.18519
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obs
0.18648
87677
99.84 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK